SIRT3 Antibody | Affinity Biosciences

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Western blot analysis of extracts from COLO205 cells, using SIRT3 antibody.

Product:	SIRT3 Antibody
Catalog:	DF4684
Description:	Rabbit polyclonal antibody to SIRT3
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	28kD, 45KD; 44kD(Calculated).
Uniprot:	Q9NTG7
RRID:	AB_2837035

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

SIRT3 Antibody detects endogenous levels of total SIRT3.

RRID:

AB_2837035
Cite Format: Affinity Biosciences Cat# DF4684, RRID:AB_2837035.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

hSIRT 3; hSIRT3; Mitochondrial nicotinamide adenine dinucleotide dependent deacetylase; NAD dependent deacetylase sirtuin 3 mitochondrial; NAD-dependent protein deacetylase sirtuin-3, mitochondrial; Regulatory protein SIR2 homolog 3; Silent mating type information regulation 2 S.cerevisiae homolog 3; Sir 2 like 3; SIR 2 like protein 3; SIR 3; SIR2 L3; Sir2 like 3; SIR2 like protein 3; SIR2-like protein 3; SIR2L3; SIR3_HUMAN; SIRT 3; SIRT3; Sirtuin 3; Sirtuin silent mating type information regulation 2 homolog 3 (S. cerevisiae); Sirtuin type 3; Sirtuin3;

Immunogens

Immunogen:

Uniprot:

Q9NTG7(SIR3_HUMAN) >>Visit HPA database.

Gene(ID):

SIRT3(23410)

Expression:

Q9NTG7 SIR3_HUMAN:

Widely expressed.

Sequence:

Q9NTG7 SIR3_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAFWGWRAAAALRLWGRVVERVEAGGGVGPFQACGCRLVLGGRDDVSAGLRGSHGARGEPLDPARPLQRPPRPEVPRAFRRQPRAAAPSFFFSSIKGGRRSISFSVGASSVVGSGGSSDKGKLSLQDVAELIRARACQRVVVMVGAGISTPSGIPDFRSPGSGLYSNLQQYDLPYPEAIFELPFFFHNPKPFFTLAKELYPGNYKPNVTHYFLRLLHDKGLLLRLYTQNIDGLERVSGIPASKLVEAHGTFASATCTVCQRPFPGEDIRADVMADRVPRCPVCTGVVKPDIVFFGEPLPQRFLLHVVDFPMADLLLILGTSLEVEPFASLTEAVRSSVPRLLINRDLVGPLAWHPRSRDVAQLGDVVHGVESLVELLGWTEEMRDLVQRETGKLDGPDK

PTMs - Q9NTG7 As Substrate

Q9NTG7

Site	PTM Type	Enzyme	Source
R51	Methylation		Uniprot
R57	Methylation		Uniprot
S105	Phosphorylation		Uniprot
S110	Phosphorylation		Uniprot
T150	Phosphorylation	P06493 (CDK1)	Uniprot
S159	Phosphorylation	P06493 (CDK1)	Uniprot
R276	Methylation		Uniprot
T284	Phosphorylation		Uniprot
R356	Methylation		Uniprot
T391	Phosphorylation		Uniprot

Research Backgrounds

Q9NTG7_SIR3_HUMAN

Function:

NAD-dependent protein deacetylase. Activates or deactivates mitochondrial target proteins by deacetylating key lysine residues. Known targets include ACSS1, IDH, GDH, SOD2, PDHA1, LCAD, SDHA and the ATP synthase subunit ATP5PO. Contributes to the regulation of the cellular energy metabolism. Important for regulating tissue-specific ATP levels. In response to metabolic stress, deacetylates transcription factor FOXO3 and recruits FOXO3 and mitochondrial RNA polymerase POLRMT to mtDNA to promote mtDNA transcription. Acts as a regulator of ceramide metabolism by mediating deacetylation of ceramide synthases CERS1, CERS2 and CERS6, thereby increasing their activity and promoting mitochondrial ceramide accumulation (By similarity).

PTMs:

Processed by mitochondrial processing peptidase (MPP) to give a 28 kDa product. Such processing is probably essential for its enzymatic activity.

Subcellular Location:

Mitochondrion matrix.

Tissue Specificity:

Widely expressed.

Subunit Structure:

Upon metabolic stress, forms a complex composed of FOXO3, SIRT3 and mitochondrial RNA polymerase POLRMT; the complex is recruited to mtDNA in a SIRT3-dependent manner. Also forms a complex composed of FOXO3, SIRT3, TFAM and POLRMT. Interacts with NDUFA9, ACSS1, IDH2 and GDH. Interacts with PCCA.

Family&Domains:

Belongs to the sirtuin family. Class I subfamily.

Research Fields

· Human Diseases > Cancers: Overview > Central carbon metabolism in cancer. (View pathway)

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SIRT3 Antibody - #DF4684