Product: ZDHHC2 Antibody
Catalog: DF4688
Description: Rabbit polyclonal antibody to ZDHHC2
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 42 KD; 42kD(Calculated).
Uniprot: Q9UIJ5
RRID: AB_2837039

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(89%), Bovine(89%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%)
Clonality:
Polyclonal
Specificity:
ZDHHC2 Antibody detects endogenous levels of total ZDHHC2.
RRID:
AB_2837039
Cite Format: Affinity Biosciences Cat# DF4688, RRID:AB_2837039.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

DHHC-2; Palmitoyltransferase ZDHHC2; Ream; Rec; Reduced expression associated with metastasis protein; Reduced expression in cancer protein; ZDHC2_HUMAN; Zdhhc2; Zinc finger DHHC domain-containing protein 2; Zinc finger protein 372; ZNF372;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9UIJ5 ZDHC2_HUMAN:

Ubiquitously expressed. Reduced expression in colorectal cancers with liver metastasis.

Sequence:
MAPSGPGSSARRRCRRVLYWIPVVFITLLLGWSYYAYAIQLCIVSMENTGEQVVCLMAYHLLFAMFVWSYWKTIFTLPMNPSKEFHLSYAEKDLLEREPRGEAHQEVLRRAAKDLPIYTRTMSGAIRYCDRCQLIKPDRCHHCSVCDKCILKMDHHCPWVNNCVGFSNYKFFLLFLAYSLLYCLFIAATDLQYFIKFWTNGLPDTQAKFHIMFLFFAAAMFSVSLSSLFGYHCWLVSKNKSTLEAFRSPVFRHGTDKNGFSLGFSKNMRQVFGDEKKYWLLPIFSSLGDGCSFPTCLVNQDPEQASTPAGLNSTAKNLENHQFPAKPLRESQSHLLTDSQSWTESSINPGKCKAGMSNPALTMENET

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
89
Horse
89
Bovine
89
Sheep
89
Dog
89
Rabbit
89
Xenopus
67
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9UIJ5 As Substrate

Site PTM Type Enzyme
S4 Phosphorylation
S8 Phosphorylation
S9 Phosphorylation
K92 Ubiquitination
K113 Ubiquitination
T119 Phosphorylation
K257 Ubiquitination
K316 Ubiquitination
K326 Ubiquitination
S333 Phosphorylation
S341 Phosphorylation
K351 Ubiquitination
S357 Phosphorylation

Research Backgrounds

Function:

Palmitoyltransferase specific for GAP43 and DLG4/PSD95.

(Microbial infection) Promotes Chikungunya virus (CHIKV) replication by mediating viral nsp1 palmitoylation.

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Reduced expression in colorectal cancers with liver metastasis.

Family&Domains:

The DHHC domain is required for palmitoyltransferase activity.

Belongs to the DHHC palmitoyltransferase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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