FGF13 Antibody - #DF4699

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Product: FGF13 Antibody
Catalog: DF4699
Description: Rabbit polyclonal antibody to FGF13
Application: WB IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 28 KD; 28kD(Calculated).
Uniprot: Q92913
RRID: AB_2837050

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
FGF13 Antibody detects endogenous levels of total FGF13.
RRID:
AB_2837050
Cite Format: Affinity Biosciences Cat# DF4699, RRID:AB_2837050.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FGF 13; FGF 2; FGF-13; FGF13; FGF13_HUMAN; FGF2; FHF 2; FHF-2; FHF2; Fibroblast growth factor 13; Fibroblast growth factor homologous factor 2; OTTHUMP00000024143; OTTHUMP00000024144;

Immunogens

Immunogen:

A synthesized peptide derived from human FGF13, corresponding to a region within the internal amino acids.

Uniprot:

Q92913(FGF13_HUMAN) >>Visit HPA database.

Gene(ID):
FGF13(2258)
Expression:
Q92913 FGF13_HUMAN:

Ubiquitously expressed. Predominantly expressed in the nervous system.

Sequence:
MAAAIASSLIRQKRQAREREKSNACKCVSSPSKGKTSCDKNKLNVFSRVKLFGSKKRRRRRPEPQLKGIVTKLYSRQGYHLQLQADGTIDGTKDEDSTYTLFNLIPVGLRVVAIQGVQTKLYLAMNSEGYLYTSELFTPECKFKESVFENYYVTYSSMIYRQQQSGRGWYLGLNKEGEIMKGNHVKKNKPAAHFLPKPLKVAMYKEPSLHDLTEFSRSGSGTPTKSRSVSGVLNGGKSMSHNEST

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Microtubule-binding protein which directly binds tubulin and is involved in both polymerization and stabilization of microtubules (By similarity). Through its action on microtubules, may participate to the refinement of axons by negatively regulating axonal and leading processes branching (By similarity). Plays a crucial role in neuron polarization and migration in the cerebral cortex and the hippocampus (By similarity). May regulate voltage-gated sodium channels transport and function. May also play a role in MAPK signaling (By similarity). Required for the development of axonal initial segment-targeting inhibitory GABAergic synapses made by chandelier neurons (By similarity).

PTMs:

May be phosphorylated.

Subcellular Location:

Cell projection>Filopodium. Cell projection>Growth cone. Cell projection>Dendrite. Nucleus. Cytoplasm.
Note: Not secreted.

Nucleus>Nucleolus.

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitously expressed. Predominantly expressed in the nervous system.

Family&Domains:

Belongs to the heparin-binding growth factors family.

References

1). Fibroblast growth factor 18 alleviates stress-induced pathological cardiac hypertrophy in male mice. Nature Communications, 2023 (PubMed: 36871047) [IF=16.6]
2). LncRNA01103/FGF13 axis promotes the progression of bone cancer pain by remodeling ac4C modification. Research Square, 2024

Application: IF/ICC    Species: Rat    Sample:

Figure 6. Regulation of FGF13 expression by lncRNA01103 in the SDH of BCP rats. (A) Western blots for monitoring FGF13 expression changes with BCP progression. ß-Actin served as loading control. Quantifications of FGF13 intensities, normalized to ß-Actin, are shown below. Bars represent the means ± SEM of Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (B) Western blots for monitoring changes in FGF13 expression after inhibition of lncRNA01103. ß-Actin served as loading control. Quantifications of FGF13 intensities, normalized to ß-Actin, are shown below. Bars represent the means ± SEM of independent biological replicates (n = 4). Values of individual experiments are indicated as dots. P-values were calculated using the one- way ANOVA (***P < 0.001). (C) RNA pull down for monitoring lncRNA01103- probe detected Fgf13 genes. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P- values were calculated using the one-way ANOVA (**P < 0.01; ***P < 0.001). (D-E) Immunofluorescence experiments showing SDH FGF13 expression after intrathecal injection of siRNA01103 in BCP rats (D). Quantifications of FGF13 mean immunofluorescence intensity are shown E. Bars represent the means ± SEM of independent biological replicates (n = 3). Values of individual experiments are indicated as dots. P-values were calculated using the one-way ANOVA (***P < 0.001). (F) Immunofluorescence staining of FGF13 and NeuN, Iba-1, and GFAP in the SDH of BCP rats at 12 d. Arrows indicate representative examples of double- labeled cells. (G) FISH (lncRNA01103, red, FGF13, green) staining in the SDH of BCP rats on postoperative day 12.

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