LAMP1 Antibody | Affinity Biosciences

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LAMP1 Antibody - DF4806 at 1/100 staining Human gastric cancer by IHC-P.

LAMP1 Antibody - DF4806 at 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P.

LAMP1 Antibody - DF4806 at 1/100 staining Mouse heart tissue by IHC-P.

LAMP1 Antibody - DF4806 at 1/100 staining Mouse muscle tissue by IHC-P.

LAMP1 Antibody - DF4806 at 1/100 staining Rat heart tissue by IHC-P.

LAMP1 Antibody - Fig.

$Figure 1: ATP7B distribution at the TGN and endolysosomes in different copper treatments. (A) Immunofluorescence image of ATP7B (green) in HepG2 cell-co-stained with endolysosomal marker LAMP2 (red) and TGN marker TGN46 (blue) shows presence of ATP7B on both compartments, irrespective of cellular copper condition i.e., basal, high copper (100µM Cu) as well as copper chelated conditions (100µM BCS). The marked area on ‘merge’ panel is enlarged in ‘inset’. The arrowhead shows colocalization between ATP7B21 LAMP2, the asterisk shows colocalization between ATP7B-TGN46. (B) Pearson’s Colocalization Coefficient (PCC) between ATP7B-TGN46 and ATP7B-LAMP2 in different copper conditions are demonstrated by box plot with jitter points. The black * depicts the comparison of colocalization between ATP7B-TGN46 and ATP7B-LAMP2. The green * shows the comparison of ATP7B-TGN46 colocalization under different copper conditions w.r.t. basal condition. The orange * shows the comparison of ATP7B-LAMP2 colocalization under different copper conditions w.r.t. basal condition. (C) Cumulative fraction of ATP7B on TGN46 and LAMP2 positive compartment are demonstrated by box plot with jitter points. The statistical comparison is done w.r.t. Basal. Sample size (N) for (B) and (C) are TTM: 42, BCS: 57, Basal: 24, Cu20: 44, Cu100: 49, Cu250: 50. (D) comparison of copper accumulation in HepG2 cells (n = 9) under different treatment conditions shows elevated copper accumulation in case of increased copper treatment, copper concentrations were measured in parts per billion (ppb). Data is demonstrated as bar-plot (mean ± SD). Statistical comparison is done w.r.t basal. (E) Comparison of copper accumulation in HepG2 cells (n = 9) under prolong copper chelated conditions (12h as well as 72h chronic chelation using BCS) shows decreased copper accumulation. Copper concentrations were measured in parts per billion (ppb). Data is demonstrated as bar-plot (mean ± SD). (F) Immunofluorescence image of ATP7B (green) in HepG2 cell line co-stained with endolysosomal marker LAMP2 (red) and TGN marker TGN46 (blue) shows presence of ATP7B on both the compartments even under chronic copper chelated condition. The marked area on ‘merge’ panel is enlarged in ‘inset’. The arrowhead shows colocalization between ATP7B-LAMP2, the asterisk shows colocalization between ATP7B-TGN46. (G) Immunofluorescenc image of ATP7B (green) co-stained with LAMP2 (red) in mouse tissue shows presence of ATP7B on LAMP2 positive compartment (marked by arrowhead). (H) Immuno-EM of endogenous ATP7B in HepG2 cell show presence of ATP7B at the Golgi membrane (marked by arrow) as well as late-endosome/lysosome compartmen (marked by arrowhead) both in BCS treated (left panel) and basal (right panel) conditions. (I) Immunoblot of ATP7B, lysosomal marker LAMP2, TGN marker GGA2 and TGN46, early endosome marker EEA1 and late endosome marker Rab7 of the top 8 fractions of sucrose gradient shows presence of ATP7B on TGN and lysosome enriched fraction. The line plot shows abundance of ATP7B (green), GGA2 (orange) and LAMP2 (violet) in 8 fractions of sucrose gradient. The ribbon plot signifies the standard deviation of 4 independent experiments. [scale bar for EM: 100 nm, for immunofluorescence: 5µm]$

LAMP1 Antibody - Figure 1: ATP7B distribution at the TGN and endolysosomes in different copper treatments.

Product:	LAMP1 Antibody
Catalog:	DF4806
Description:	Rabbit polyclonal antibody to LAMP1
Application:	WB IHC IF/ICC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Rabbit, Dog
Mol.Wt.:	45kDa,130kDa(Glycosylated)(Observed); 45kD(Calculated).
Uniprot:	P11279
RRID:	AB_2837099

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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200ul	$350	In stock

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Rabbit(82%), Dog(91%)

Clonality:

Polyclonal

Specificity:

LAMP1 Antibody detects endogenous levels of total LAMP1.

RRID:

AB_2837099
Cite Format: Affinity Biosciences Cat# DF4806, RRID:AB_2837099.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

CD107 antigen like family member A; CD107 antigen-like family member A; CD107a; CD107a antigen; LAMP 1; LAMP-1; LAMP1; LAMP1_HUMAN; LAMPA; LGP120; lgpA; Lysosomal membrane glycoprotein 120KD; Lysosomal Associated Membrane Protein 1; Lysosome associated membrane glycoprotein 1; Lysosome-associated membrane glycoprotein 1; Lysosome-associated membrane protein 1; OTTHUMP00000040663;

Immunogens

Immunogen:

A synthesized peptide derived from human LAMP1, corresponding to a region within the internal amino acids.

Uniprot:

P11279(LAMP1_HUMAN) >>Visit HPA database.

Gene(ID):

LAMP1(3916)

Sequence:

P11279 LAMP1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MAAPGSARRPLLLLLLLLLLGLMHCASAAMFMVKNGNGTACIMANFSAAFSVNYDTKSGPKNMTFDLPSDATVVLNRSSCGKENTSDPSLVIAFGRGHTLTLNFTRNATRYSVQLMSFVYNLSDTHLFPNASSKEIKTVESITDIRADIDKKYRCVSGTQVHMNNVTVTLHDATIQAYLSNSSFSRGETRCEQDRPSPTTAPPAPPSPSPSPVPKSPSVDKYNVSGTNGTCLLASMGLQLNLTYERKDNTTVTRLLNINPNKTSASGSCGAHLVTLELHSEGTTVLLFQFGMNASSSRFFLQGIQLNTILPDARDPAFKAANGSLRALQATVGNSYKCNAEEHVRVTKAFSVNIFKVWVQAFKVEGGQFGSVEECLLDENSMLIPIAVGGALAGLVLIVLIAYLVGRKRSHAGYQTI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Rabbit

Pig

Horse

Bovine

Sheep

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

P11279_LAMP1_HUMAN

Function:

Presents carbohydrate ligands to selectins. Also implicated in tumor cell metastasis.

Acts as a receptor for Lassa virus protein.

PTMs:

O- and N-glycosylated; some of the 18 N-linked glycans are polylactosaminoglycans. The glycosylation of N-76 is essential for Lassa virus entry into cells.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Endosome membrane>Single-pass type I membrane protein. Lysosome membrane>Single-pass type I membrane protein. Late endosome.
Note: This protein shuttles between lysosomes, endosomes, and the plasma membrane (By similarity). Colocalizes with OSBPL1A at the late endosome (PubMed:16176980).

Family&Domains:

Belongs to the LAMP family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome. (View pathway)

· Cellular Processes > Transport and catabolism > Phagosome. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Tuberculosis.

References

1). ABHD5 blunts the sensitivity of colorectal cancer to fluorouracil via promoting autophagic uracil yield. Nature Communications, 2019 (PubMed: 30842415) [IF=16.6]

Application: WB Species: human Sample: SW480 cells

Fig. 5| ABHD5 sustains the activity of RNASET2. a Representative images of immunofluorescent stainings of lysosome (stained by Lysotracker, red) and ABHD5 (green) in SW480 cells. Scale bar: 10 μm. b Western blots showing ABHD5 expression in the lysosome lysates of ABHD5 KD and control cells. LAMP1 was used as a reference.

2). Identification of walnut-derived peptide WNP-8 as a potential therapeutic agent and SIRT2 as a biomarker for cognitive deficiency: A label-free LC-MS/MS proteomics study. International journal of biological macromolecules, 2024 (PubMed: 39647753) [IF=7.7]

3). Transcranial direct current stimulation exerts neuroprotective effects in Parkinson's disease by restoring Mlst8-mediated autophagic homeostasis. Journal of translational medicine, 2026 (PubMed: 41484879) [IF=7.4]

4). Palmatine activation of TFEB enhances autophagy and alleviates endoplasmic reticulum stress in intervertebral disc degeneration. Phytomedicine : international journal of phytotherapy and phytopharmacology, 2025 (PubMed: 39933468) [IF=6.7]

5). Formononetin derived from Parabacteroides merdae alleviates MPTP-induced Parkinson's disease in mice by inhibiting ferroptosis via the PI3K-AKT-ferritinophagy axis. Communications biology, 2025 (PubMed: 41291228) [IF=5.9]

6). PPM1D ameliorates Alzheimer's disease by promoting mitophagy. Experimental neurology, 2025 (PubMed: 40090398) [IF=4.6]

7). Copper-independent lysosomal localisation of the Wilson disease protein ATP7B. Traffic (Copenhagen, Denmark), 2023 (PubMed: 37846526) [IF=3.6]

8). Regulation of the apico-basolateral trafficking polarity of the homologous copper-ATPases ATP7A and ATP7B. Journal of cell science, 2023 (PubMed: 38032054) [IF=3.3]

9). Dexmedetomidine Blocks NCOA4-Dependent Ferritinophagy to Confer Ferroptosis Resistance in Lung Ischemia Reperfusion Injury via Targeting NRF2. Journal of biochemical and molecular toxicology, 2025 (PubMed: 39887555) [IF=3.2]

10). CTRP3 alleviates cardiac ischemia/reperfusion injury via LAMP1/JIP2/JNK signaling pathway. Imaging, 2022 (PubMed: 35114641) [IF=0.4]

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LAMP1 Antibody - #DF4806