Product: MERTK Antibody
Catalog: DF4785
Description: Rabbit polyclonal antibody to MERTK
Application: WB IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.: 110KDa, 130~180kDa(Glycosylation); 110kD(Calculated).
Uniprot: Q12866
RRID: AB_2837139

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
MERTK Antibody detects endogenous levels of total MERTK.
RRID:
AB_2837139
Cite Format: Affinity Biosciences Cat# DF4785, RRID:AB_2837139.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

c MER; c mer proto oncogene tyrosine kinase; c-mer; cMER; cmer protooncogene tyrosine kinase; Eyk; MER; MER receptor tyrosine kinase; MERK; MERPEN; Mertk; MERTK c-mer proto-oncogene tyrosine kinase; MERTK_HUMAN; MGC133349; nmf12; Nyk; Proto oncogene tyrosine protein kinase MER; Proto oncogene tyrosine protein kinase MER precursor; Proto-oncogene c-Mer; Receptor tyrosine kinase MerTK; RP38; STK kinase; Tyrosine-protein kinase Mer;

Immunogens

Immunogen:

A synthesized peptide derived from human MERTK, corresponding to a region within the internal amino acids.

Uniprot:
Gene(ID):
Expression:
Q12866 MERTK_HUMAN:

Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Sequence:
MGPAPLPLLLGLFLPALWRRAITEAREEAKPYPLFPGPFPGSLQTDHTPLLSLPHASGYQPALMFSPTQPGRPHTGNVAIPQVTSVESKPLPPLAFKHTVGHIILSEHKGVKFNCSISVPNIYQDTTISWWKDGKELLGAHHAITQFYPDDEVTAIIASFSITSVQRSDNGSYICKMKINNEEIVSDPIYIEVQGLPHFTKQPESMNVTRNTAFNLTCQAVGPPEPVNIFWVQNSSRVNEQPEKSPSVLTVPGLTEMAVFSCEAHNDKGLTVSKGVQINIKAIPSPPTEVSIRNSTAHSILISWVPGFDGYSPFRNCSIQVKEADPLSNGSVMIFNTSALPHLYQIKQLQALANYSIGVSCMNEIGWSAVSPWILASTTEGAPSVAPLNVTVFLNESSDNVDIRWMKPPTKQQDGELVGYRISHVWQSAGISKELLEEVGQNGSRARISVQVHNATCTVRIAAVTRGGVGPFSDPVKIFIPAHGWVDYAPSSTPAPGNADPVLIIFGCFCGFILIGLILYISLAIRKRVQETKFGNAFTEEDSELVVNYIAKKSFCRRAIELTLHSLGVSEELQNKLEDVVIDRNLLILGKILGEGEFGSVMEGNLKQEDGTSLKVAVKTMKLDNSSQREIEEFLSEAACMKDFSHPNVIRLLGVCIEMSSQGIPKPMVILPFMKYGDLHTYLLYSRLETGPKHIPLQTLLKFMVDIALGMEYLSNRNFLHRDLAARNCMLRDDMTVCVADFGLSKKIYSGDYYRQGRIAKMPVKWIAIESLADRVYTSKSDVWAFGVTMWEIATRGMTPYPGVQNHEMYDYLLHGHRLKQPEDCLDELYEIMYSCWRTDPLDRPTFSVLRLQLEKLLESLPDVRNQADVIYVNTQLLESSEGLAQGSTLAPLDLNIDPDSIIASCTPRAAISVVTAEVHDSKPHEGRYILNGGSEEWEDLTSAPSAAVTAEKNSVLPGERLVRNGVSWSHSSMLPLGSSLPDELLFADDSSEGSEVLM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to several ligands including LGALS3, TUB, TULP1 or GAS6. Regulates many physiological processes including cell survival, migration, differentiation, and phagocytosis of apoptotic cells (efferocytosis). Ligand binding at the cell surface induces autophosphorylation of MERTK on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with GRB2 or PLCG2 and induces phosphorylation of MAPK1, MAPK2, FAK/PTK2 or RAC1. MERTK signaling plays a role in various processes such as macrophage clearance of apoptotic cells, platelet aggregation, cytoskeleton reorganization and engulfment. Functions in the retinal pigment epithelium (RPE) as a regulator of rod outer segments fragments phagocytosis. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response by activating STAT1, which selectively induces production of suppressors of cytokine signaling SOCS1 and SOCS3.

PTMs:

Autophosphorylated on Tyr-749, Tyr-753 and Tyr-754 in the activation loop allowing full activity. Autophosphorylated on Tyr-872 leading to recruitment of downstream partners of the signaling cascade such as PLCG2 (By similarity).

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Not expressed in normal B- and T-lymphocytes but is expressed in numerous neoplastic B- and T-cell lines. Highly expressed in testis, ovary, prostate, lung, and kidney, with lower expression in spleen, small intestine, colon, and liver.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

References

1). Screen of FDA-approved drug library identifies vitamin K as anti-ferroptotic drug for osteoarthritis therapy through Gas6. Journal of Pharmaceutical Analysis, 2025 [IF=6.1]

2). Study on the involvement of microglial S100A8 in neuroinflammation and microglia activation during migraine attacks. Molecular and cellular neurosciences, 2024 (PubMed: 39111720) [IF=2.6]

Application: WB    Species: Rat    Sample:

Fig. 2. Validation the expression of DEGs related to microglia in the trigeminal ganglion (TG), and medulla oblongata (MO) and microglia of migraine rats. The mRNA levels (A) and protein expression (B) of MAFB, MERTK, SLCO2B1, S100A9, and S100A8 in the TG. The mRNA levels (C) and protein expression of MAFB, MERTK, SLCO2B1, S100A9, and S100A8 in the MO (D). ⁎P < 0.05, ⁎⁎P < 0.01, ⁎⁎⁎P < 0.001 vs. Control.

3). Protective Role of MerTK in Diabetic Peripheral Neuropathy via Inhibition of the NF-κB Signaling Pathway. Experimental and clinical endocrinology & diabetes : official journal, German Society of Endocrinology [and] German Diabetes Association, 2024 (PubMed: 38588709)

Application: IF/ICC    Species: Rat    Sample:

Fig. 2 Immunofluorescence detection of MerTK expression in each group. Confocal images of MerTK (green) and DAPI (blue) staining of the nucleus. Scale bar=500 μm (first column). Scale bar=50 μm (columns 2, 3, and 4). Rat counts per group were: CON: 14, CON+MRX-2843: 14, T2DM: 10, DPN: 12, DPN+MRX-2843: 10, with a uniform age of 96 weeks. Sciatic nerves served as the study tissue. MerTK, mer tyrosine kinase; CON, control group; MRX, MerTK-specific inhibitor; T2DM, type 2 diabetes mellitus; DPN, diabetic peripheral neuropathy.|

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