Product: ETV4 Antibody
Catalog: BF0482
Description: Mouse monoclonal antibody to ETV4
Application: WB ELISA
Reactivity: Human
Mol.Wt.: 54kDa; 54kD(Calculated).
Uniprot: P43268
RRID: AB_2833650

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Product Info

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1626]
ETV4 antibody detects endogenous levels of total ETV4.
Cite Format: Affinity Biosciences Cat# BF0482, RRID:AB_2833650.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Adenovirus E1A enhancer binding protein; Adenovirus E1A enhancer-binding protein; E1A F; E1A-F; ETS translocation variant 4; ets variant gene 4 (E1A enhancer binding protein E1AF); ETV4; ETV4_HUMAN; PEA3; PEA3 protein; PEAS3; Polyomavirus enhancer activator 3; Polyomavirus enhancer activator 3 homolog; Protein PEA3;



Purified recombinant fragment of human ETV4 expressed in E. Coli.

ETV4: ets variant gene 4 (E1A enhancer binding protein, E1AF), also known as PEA3. Several members of the Ets gene family are known to encode sequencespecific DNA binding proteins. These include mouse PU.1, mouse and human Ets-1, Drosophila E74, chicken and human Ets-2 and rat GABP-α. Each of these proteins recognizes similar motifs in DNA that share a centrally located 5'-GGAA-3' element. For instance, PEA3 binds the motif 5'-AGGAAG-3' (the PEA-3 motif), but does not bind to the sequence 5'-AGGAAC-3', recognized by PU.1, although PU.1 binds equally well to both sequences. It appears that all of the Ets proteins recognize the same central core sequence but that each protein interacts with unique sequences that flank this core. PEA3 is expressed at readily detectable levels in cells of epithelial and fibroblastic origin but is not expressed in hematopoietic cells. This is in contrast to other members of the Ets gene family, such as Ets-1, Ets-2 and Fli-1, each of which is expressed primarily in cells of hematopoietic origin.

PTMs - P43268 As Substrate

Site PTM Type Enzyme
S73 Phosphorylation
K96 Sumoylation
S101 Phosphorylation
S140 Phosphorylation
S149 Phosphorylation
K226 Sumoylation
K260 Sumoylation
K322 Sumoylation
Y392 Phosphorylation
S396 Phosphorylation Q15831 (STK11)
Y416 Phosphorylation
K441 Sumoylation

Research Backgrounds


Transcriptional activator that binds to the enhancer of the adenovirus E1A gene; the core-binding sequence is 5'[AC]GGA[AT]GT-3'.


Sumoylated; enhanced upon ERK/MAP kinase pathway activation, it positively regulates the transcriptional activator capacity. Sumoylation at Lys-96 probably requires phosphorylation at Ser-101. Transiently polysumoylated and desumoylated by SENP1. Sumoylation is a prerequisite to polyubiquitination which in turn increases proteasomal-mediated degradation. Probably polyubiquitinated by RNF4 and deubiquitinated by USP2.

Subcellular Location:


Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Belongs to the ETS family.

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

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