STK33 Antibody - #DF4791

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Product: STK33 Antibody
Catalog: DF4791
Description: Rabbit polyclonal antibody to STK33
Application: WB IHC IF/ICC
Reactivity: Human
Prediction: Bovine, Horse, Sheep, Dog
Mol.Wt.: 58 KD; 58kD(Calculated).
Uniprot: Q9BYT3
RRID: AB_2837145

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Bovine(92%), Horse(92%), Sheep(85%), Dog(85%)
Clonality:
Polyclonal
Specificity:
STK33 Antibody detects endogenous levels of total STK33.
RRID:
AB_2837145
Cite Format: Affinity Biosciences Cat# DF4791, RRID:AB_2837145.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Hypothetical protein FLJ35932; Serine threonine kinase 33; Serine/threonine kinase 33; Serine/threonine protein kinase 33; Serine/threonine-protein kinase 33; STK 33; Stk33; STK33_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q9BYT3(STK33_HUMAN) >>Visit HPA database.

Gene(ID):
STK33(65975)
Expression:
Q9BYT3 STK33_HUMAN:

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.

Sequence:
MADSGLDKKSTKCPDCSSASQKDVLCVCSSKTRVPPVLVVEMSQTSSIGSAESLISLERKKEKNINRDITSRKDLPSRTSNVERKASQQQWGRGNFTEGKVPHIRIENGAAIEEIYTFGRILGKGSFGIVIEATDKETETKWAIKKVNKEKAGSSAVKLLEREVNILKSVKHEHIIHLEQVFETPKKMYLVMELCEDGELKEILDRKGHFSENETRWIIQSLASAIAYLHNNDIVHRDLKLENIMVKSSLIDDNNEINLNIKVTDFGLAVKKQSRSEAMLQATCGTPIYMAPEVISAHDYSQQCDIWSIGVVMYMLLRGEPPFLASSEEKLFELIRKGELHFENAVWNSISDCAKSVLKQLMKVDPAHRITAKELLDNQWLTGNKLSSVRPTNVLEMMKEWKNNPESVEENTTEEKNKPSTEEKLKSYQPWGNVPDANYTSDEEEEKQSTAYEKQFPATSKDNFDMCSSSFTSSKLLPAEIKGEMEKTPVTPSQGTATKYPAKSGALSRTKKKL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
92
Bovine
92
Sheep
85
Dog
85
Rabbit
77
Pig
75
Zebrafish
67
Xenopus
58
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9BYT3 As Substrate

Site PTM Type Enzyme
K31 Ubiquitination
K73 Acetylation
K85 Ubiquitination
S87 Phosphorylation
K100 Ubiquitination
Y116 Phosphorylation
K141 Ubiquitination
S154 Phosphorylation
S155 Phosphorylation
K158 Ubiquitination
K168 Ubiquitination
K240 Ubiquitination
S301 Phosphorylation
S356 Phosphorylation
K359 Ubiquitination
K373 Ubiquitination
K385 Ubiquitination
K402 Ubiquitination
S420 Phosphorylation
T421 Phosphorylation
T440 Phosphorylation
S441 Phosphorylation
K454 Ubiquitination
S469 Phosphorylation
S470 Phosphorylation
T491 Phosphorylation
S493 Phosphorylation
T496 Phosphorylation
Y500 Phosphorylation
S504 Phosphorylation
S508 Phosphorylation
T510 Phosphorylation

Research Backgrounds

Function:

Serine/threonine protein kinase which phosphorylates VIME. May play a specific role in the dynamic behavior of the intermediate filament cytoskeleton by phosphorylation of VIME (By similarity). Not essential for the survival of KRAS-dependent AML cell lines.

PTMs:

Autophosphorylated.

Subcellular Location:

Cytoplasm>Perinuclear region.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in testis, fetal lung and heart, followed by pituitary gland, kidney, interventricular septum, pancreas, heart, trachea, thyroid gland and uterus. Weak hybridization signals were observed in the following tissues: amygdala, aorta, esophagus, colon ascending, colon transverse, skeletal muscle, spleen, peripheral blood leukocyte, lymph node, bone marrow, placenta, prostate, liver, salivary gland, mammary gland, some tumor cell lines, fetal brain, fetal liver, fetal spleen and fetal thymus. No signal at all was detectable in RNA from tissues of the nervous system.

Subunit Structure:

Interacts with VIME.

Family&Domains:

Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. CaMK subfamily.

Restrictive clause

 

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