Product: TESK2 Antibody
Catalog: DF4795
Description: Rabbit polyclonal antibody to TESK2
Application: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit
Mol.Wt.: 65 KD; 64kD(Calculated).
Uniprot: Q96S53
RRID: AB_2837149

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(91%), Horse(100%), Sheep(91%), Rabbit(89%)
Clonality:
Polyclonal
Specificity:
TESK2 Antibody detects endogenous levels of total TESK2.
RRID:
AB_2837149
Cite Format: Affinity Biosciences Cat# DF4795, RRID:AB_2837149.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Dual specificity testis specific protein kinase 2; Dual specificity testis-specific protein kinase 2; MGC29168; OTTHUMP00000009093; RP23-109A3.2; Tesk2; TESK2_HUMAN; Testicular protein kinase 2; Testis specific kinase 2;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q96S53 TESK2_HUMAN:

Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.

Sequence:
MDRSKRNSIAGFPPRVERLEEFEGGGGGEGNVSQVGRVWPSSYRALISAFSRLTRLDDFTCEKIGSGFFSEVFKVRHRASGQVMALKMNTLSSNRANMLKEVQLMNRLSHPNILRFMGVCVHQGQLHALTEYINSGNLEQLLDSNLHLPWTVRVKLAYDIAVGLSYLHFKGIFHRDLTSKNCLIKRDENGYSAVVADFGLAEKIPDVSMGSEKLAVVGSPFWMAPEVLRDEPYNEKADVFSYGIILCEIIARIQADPDYLPRTENFGLDYDAFQHMVGDCPPDFLQLTFNCCNMDPKLRPSFVEIGKTLEEILSRLQEEEQERDRKLQPTARGLLEKAPGVKRLSSLDDKIPHKSPCPRRTIWLSRSQSDIFSRKPPRTVSVLDPYYRPRDGAARTPKVNPFSARQDLMGGKIKFFDLPSKSVISLVFDLDAPGPGTMPLADWQEPLAPPIRRWRSLPGSPEFLHQEACPFVGREESLSDGPPPRLSSLKYRVKEIPPFRASALPAAQAHEAMDCSILQEENGFGSRPQGTSPCPAGASEEMEVEERPAGSTPATFSTSGIGLQTQGKQDG

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
91
Sheep
91
Rabbit
89
Pig
82
Dog
73
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96S53 As Substrate

Site PTM Type Enzyme
S8 Phosphorylation
K180 Ubiquitination
K326 Ubiquitination
S346 Phosphorylation
S367 Phosphorylation
S369 Phosphorylation
S381 Phosphorylation
K412 Ubiquitination
S456 Phosphorylation
S460 Phosphorylation
S477 Phosphorylation
S488 Phosphorylation

PTMs - Q96S53 As Enzyme

Substrate Site Source
P23528 (CFL1) S3 Uniprot
P60981 (DSTN) S3 Uniprot
Q9Y281 (CFL2) S3 Uniprot

Research Backgrounds

Function:

Dual specificity protein kinase activity catalyzing autophosphorylation and phosphorylation of exogenous substrates on both serine/threonine and tyrosine residues. Phosphorylates cofilin at 'Ser-3'. May play an important role in spermatogenesis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Predominantly expressed in testis and prostate. Found predominantly in non-germinal Sertoli cells.

Family&Domains:

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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