VRK3 Antibody - #DF4796

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Product: VRK3 Antibody
Catalog: DF4796
Description: Rabbit polyclonal antibody to VRK3
Application: WB IF/ICC
Reactivity: Human
Prediction: Dog
Mol.Wt.: 52 KD; 53kD(Calculated).
Uniprot: Q8IV63
RRID: AB_2837150

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Related Downloads
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Dog(100%)
Clonality:
Polyclonal
Specificity:
VRK3 Antibody detects endogenous levels of total VRK3.
RRID:
AB_2837150
Cite Format: Affinity Biosciences Cat# DF4796, RRID:AB_2837150.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Inactive serine/threonine-protein kinase VRK3; Serine/threonine protein kinase VRK3; Serine/threonine-protein pseudokinase VRK3; Vaccinia related kinase 3; Vaccinia-related kinase 3; Vrk3; VRK3_HUMAN;

Immunogens

Immunogen:
Uniprot:

Q8IV63(VRK3_HUMAN) >>Visit HPA database.

Gene(ID):
VRK3(51231)
Sequence:
MISFCPDCGKSIQAAFKFCPYCGNSLPVEEHVGSQTFVNPHVSSFQGSKRGLNSSFETSPKKVKWSSTVTSPRLSLFSDGDSSESEDTLSSSERSKGSGSRPPTPKSSPQKTRKSPQVTRGSPQKTSCSPQKTRQSPQTLKRSRVTTSLEALPTGTVLTDKSGRQWKLKSFQTRDNQGILYEAAPTSTLTCDSGPQKQKFSLKLDAKDGRLFNEQNFFQRAAKPLQVNKWKKLYSTPLLAIPTCMGFGVHQDKYRFLVLPSLGRSLQSALDVSPKHVLSERSVLQVACRLLDALEFLHENEYVHGNVTAENIFVDPEDQSQVTLAGYGFAFRYCPSGKHVAYVEGSRSPHEGDLEFISMDLHKGCGPSRRSDLQSLGYCMLKWLYGFLPWTNCLPNTEDIMKQKQKFVDKPGPFVGPCGHWIRPSETLQKYLKVVMALTYEEKPPYAMLRNNLEALLQDLRVSPYDPIGLPMVP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Dog
100
Bovine
75
Pig
63
Horse
63
Sheep
63
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IV63 As Substrate

Site PTM Type Enzyme
R50 Methylation
S54 Phosphorylation
S55 Phosphorylation
T58 Phosphorylation
S59 Phosphorylation
S66 Phosphorylation
S67 Phosphorylation
T70 Phosphorylation
S71 Phosphorylation
S82 Phosphorylation
S83 Phosphorylation
S85 Phosphorylation
S90 Phosphorylation
S95 Phosphorylation
S98 Phosphorylation
T104 Phosphorylation
S107 Phosphorylation
S108 Phosphorylation
T112 Phosphorylation
S115 Phosphorylation
T119 Phosphorylation
S122 Phosphorylation
T126 Phosphorylation
S129 Phosphorylation
S136 Phosphorylation
T154 Phosphorylation
T156 Phosphorylation
T159 Phosphorylation
K161 Ubiquitination
K169 Ubiquitination
K197 Ubiquitination
K223 Ubiquitination
K229 Ubiquitination
K232 Ubiquitination
K275 Acetylation
K275 Ubiquitination
K338 Ubiquitination
K410 Ubiquitination

PTMs - Q8IV63 As Enzyme

Substrate Site Source
O75531 (BANF1) S4 Uniprot

Research Backgrounds

Function:

Inactive kinase that suppresses ERK activity by promoting phosphatase activity of DUSP3 which specifically dephosphorylates and inactivates ERK in the nucleus.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with DUSP3 (By similarity). Interacts with RAN.

Family&Domains:

Belongs to the protein kinase superfamily. CK1 Ser/Thr protein kinase family. VRK subfamily.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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