ERI1 Antibody - #DF4800

(5)  
Product: ERI1 Antibody
Catalog: DF4800
Description: Rabbit polyclonal antibody to ERI1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 40 KD; 40kD(Calculated).
Uniprot: Q8IV48
RRID: AB_2837154

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
IHC 1:50-1:200, WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(89%), Zebrafish(100%), Horse(89%), Sheep(89%), Rabbit(89%), Dog(89%), Chicken(89%)
Clonality:
Polyclonal
Specificity:
ERI1 Antibody detects endogenous levels of total ERI1.
RRID:
AB_2837154
Cite Format: Affinity Biosciences Cat# DF4800, RRID:AB_2837154.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3' 5' exonuclease ERI 1; 3' 5' exonuclease ERI1; 3' EXO; 3' exoribonuclease; 3' HEXO; 3' histone mRNA exonuclease 1; 3''-5'' exonuclease eri1; 3''-5'' exoribonuclease 1; 3'EXO; 3'HEXO; ERI 1; Eri 1 homolog; Eri-1 homolog; eri1; Eri1 homolog; ERI1_HUMAN; HEXO; Histone mRNA 3' end specific exonuclease; Histone mRNA 3' end specific exoribonuclease; Histone mRNA 3''-end-specific exoribonuclease; Histone mRNA 3''-exonuclease 1; MGC35395; Protein 3''hExo; THEX 1; THEX1; Three prime histone mRNA exonuclease 1;

Immunogens

Immunogen:
Uniprot:

Q8IV48(ERI1_HUMAN) >>Visit HPA database.

Gene(ID):
ERI1(90459)
Sequence:
MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASDFSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
89
Horse
89
Sheep
89
Dog
89
Chicken
89
Rabbit
89
Xenopus
67
Bovine
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8IV48 As Substrate

Site PTM Type Enzyme
S21 Phosphorylation
S34 Phosphorylation
K49 Sumoylation
K49 Ubiquitination
K52 Sumoylation
K52 Ubiquitination
S57 Phosphorylation
S59 Phosphorylation
S62 Phosphorylation
Y66 Phosphorylation
K67 Acetylation
K67 Ubiquitination
K81 Sumoylation
K81 Ubiquitination
K87 Ubiquitination
K92 Ubiquitination
K99 Acetylation
K222 Ubiquitination
K249 Ubiquitination
K262 Ubiquitination
K269 Ubiquitination
K276 Ubiquitination
K300 Ubiquitination

Research Backgrounds

Function:

RNA exonuclease that binds to the 3'-end of histone mRNAs and degrades them, suggesting that it plays an essential role in histone mRNA decay after replication. A 2' and 3'-hydroxyl groups at the last nucleotide of the histone 3'-end is required for efficient degradation of RNA substrates. Also able to degrade the 3'-overhangs of short interfering RNAs (siRNAs) in vitro, suggesting a possible role as regulator of RNA interference (RNAi). Requires for binding the 5'-ACCCA-3' sequence present in stem-loop structure. Able to bind other mRNAs. Required for 5.8S rRNA 3'-end processing. Also binds to 5.8s ribosomal RNA. Binds with high affinity to the stem-loop structure of replication-dependent histone pre-mRNAs.

Subcellular Location:

Cytoplasm. Nucleus. Nucleus>Nucleolus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Identified in a histone pre-mRNA complex, at least composed of ERI1, LSM11, SLBP, SNRPB, SYNCRIP and YBX1. Interacts in a cooperative manner with SLBP to the mature 3'-end of histone mRNAs (By similarity). Binds to 40S and 60S ribosomal subunits and to 80S assembled ribosomes. Found in a ternary complex with SLBP and the stem-loop structure of the 3'-end of histone mRNAs.

Family&Domains:

The SAP domain is necessary for binding to the stem-loop structure of histone mRNAs and to form the ternary complex with SLBP, but not for 3'-end histone mRNA exonuclease activity.

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