FPR1 Antibody | Affinity Biosciences

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Western blot analysis of extracts from K562 cells, using FPR1 antibody.

Product:	FPR1 Antibody
Catalog:	DF4881
Description:	Rabbit polyclonal antibody to FPR1
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	40 KD; 38kD(Calculated).
Uniprot:	P21462
RRID:	AB_2837173

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

FPR1 Antibody detects endogenous levels of total FPR1.

RRID:

AB_2837173
Cite Format: Affinity Biosciences Cat# DF4881, RRID:AB_2837173.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

fMet Leu Phe receptor; fMet-Leu-Phe receptor; FMLP; fMLP receptor; FMLPR; Formyl peptide receptor 1; FPR 1; FPR; FPR receptor; FPR1; FPR1_HUMAN; N formyl peptide chemoattractant receptor; N formyl peptide receptor; N formylpeptide chemoattractant receptor; N-formyl peptide receptor; N-formylpeptide chemoattractant receptor;

Immunogens

Immunogen:

Uniprot:

P21462(FPR1_HUMAN) >>Visit HPA database.

Gene(ID):

FPR1(2357)

Expression:

P21462 FPR1_HUMAN:

Neutrophils.

Sequence:

P21462 FPR1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

METNSSLPTNISGGTPAVSAGYLFLDIITYLVFAVTFVLGVLGNGLVIWVAGFRMTHTVTTISYLNLAVADFCFTSTLPFFMVRKAMGGHWPFGWFLCKFVFTIVDINLFGSVFLIALIALDRCVCVLHPVWTQNHRTVSLAKKVIIGPWVMALLLTLPVIIRVTTVPGKTGTVACTFNFSPWTNDPKERINVAVAMLTVRGIIRFIIGFSAPMSIVAVSYGLIATKIHKQGLIKSSRPLRVLSFVAAAFFLCWSPYQVVALIATVRIRELLQGMYKEIGIAVDVTSALAFFNSCLNPMLYVFMGQDFRERLIHALPASLERALTEDSTQTSDTATNSTLPSAEVELQAK

PTMs - P21462 As Substrate

P21462

Site	PTM Type	Enzyme	Source
Y22	Phosphorylation		Uniprot
S211	Phosphorylation		Uniprot
S215	Phosphorylation		Uniprot
S220	Phosphorylation		Uniprot
T325	Phosphorylation		Uniprot
S328	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
T329	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
T331	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
S332	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
T334	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
T336	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
S338	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
T339	Phosphorylation	A0A0S2Z3I6 (ADRBK1)	Uniprot
S342	Phosphorylation	Q16539 (MAPK14)	Uniprot

Research Backgrounds

P21462_FPR1_HUMAN

Function:

High affinity receptor for N-formyl-methionyl peptides (fMLP), which are powerful neutrophil chemotactic factors. Binding of fMLP to the receptor stimulates intracellular calcium mobilization and superoxide anion release. This response is mediated via a G-protein that activates a phosphatidylinositol-calcium second messenger system. Receptor for TAFA4, mediates its effects on chemoattracting macrophages, promoting phagocytosis and increasing ROS release.

PTMs:

Phosphorylated; which is necessary for desensitization.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.
Note: Internalizes in presence of its ligand, TAFA4.

Tissue Specificity:

Neutrophils.

Subunit Structure:

Interacts with S.aureus chemotaxis inhibitory protein (CHIPS); the interaction blocks the receptor and may thus inhibit the immune response.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway. (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Infectious diseases: Bacterial > Staphylococcus aureus infection.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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FPR1 Antibody - #DF4881