MCPH1 Antibody - #DF4831
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Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4831, RRID:AB_2837196.
Fold/Unfold
BRCT repeat inhibitor of TERT expression 1; BRIT 1; FLJ12847; Hypothetical protein FLJ12847; MCPH 1; MCPH1; MCPH1_HUMAN; MCT; Microcephalin 1; Microcephalin; Microcephaly primary autosomal recessive 1;
Immunogens
- Q8NEM0 MCPH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAAPILKDVVAYVEVWSSNGTENYSKTFTTQLVDMGAKVSKTFNKQVTHVIFKDGYQSTWDKAQKRGVKLVSVLWVEKCRTAGAHIDESLFPAANMNEHLSSLIKKKRKCMQPKDFNFKTPENDKRFQKKFEKMAKELQRQKTNLDDDVPILLFESNGSLIYTPTIEINSRHHSAMEKRLQEMKEKRENLSPTSSQMIQQSHDNPSNSLCEAPLNISRDTLCSDEYFAGGLHSSFDDLCGNSGCGNQERKLEGSINDIKSDVCISSLVLKANNIHSSPSFTHLDKSSPQKFLSNLSKEEINLQRNIAGKVVTPDQKQAAGMSQETFEEKYRLSPTLSSTKGHLLIHSRPRSSSVKRKRVSHGSHSPPKEKCKRKRSTRRSIMPRLQLCRSEDRLQHVAGPALEALSCGESSYDDYFSPDNLKERYSENLPPESQLPSSPAQLSCRSLSKKERTSIFEMSDFSCVGKKTRTVDITNFTAKTISSPRKTGNGEGRATSSCVTSAPEEALRCCRQAGKEDACPEGNGFSYTIEDPALPKGHDDDLTPLEGSLEEMKEAVGLKSTQNKGTTSKISNSSEGEAQSEHEPCFIVDCNMETSTEEKENLPGGYSGSVKNRPTRHDVLDDSCDGFKDLIKPHEELKKSGRGKKPTRTLVMTSMPSEKQNVVIQVVDKLKGFSIAPDVCETTTHVLSGKPLRTLNVLLGIARGCWVLSYDWVLWSLELGHWISEEPFELSHHFPAAPLCRSECHLSAGPYRGTLFADQPAMFVSPASSPPVAKLCELVHLCGGRVSQVPRQASIVIGPYSGKKKATVKYLSEKWVLDSITQHKVCAPENYLLSQ
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q8NEM0 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K38 | Ubiquitination | Uniprot | |
| K62 | Ubiquitination | Uniprot | |
| S102 | Phosphorylation | Uniprot | |
| K105 | Ubiquitination | Uniprot | |
| K119 | Ubiquitination | Uniprot | |
| T120 | Phosphorylation | Uniprot | |
| T143 | Phosphorylation | Uniprot | |
| S191 | Phosphorylation | Uniprot | |
| K250 | Ubiquitination | Uniprot | |
| S254 | Phosphorylation | Uniprot | |
| S276 | Phosphorylation | Uniprot | |
| S277 | Phosphorylation | Uniprot | |
| S279 | Phosphorylation | Uniprot | |
| T281 | Phosphorylation | Uniprot | |
| S287 | Phosphorylation | Uniprot | |
| K297 | Ubiquitination | Uniprot | |
| K309 | Acetylation | Uniprot | |
| K309 | Ubiquitination | Uniprot | |
| T312 | Phosphorylation | Uniprot | |
| S322 | Phosphorylation | Uniprot | |
| K329 | Ubiquitination | Uniprot | |
| S333 | Phosphorylation | Uniprot | |
| T335 | Phosphorylation | Uniprot | |
| S337 | Phosphorylation | Uniprot | |
| S338 | Phosphorylation | Uniprot | |
| T339 | Phosphorylation | Uniprot | |
| S365 | Phosphorylation | Uniprot | |
| S406 | Phosphorylation | Uniprot | |
| K422 | Ubiquitination | Uniprot | |
| S437 | Phosphorylation | Uniprot | |
| S438 | Phosphorylation | Uniprot | |
| T543 | Phosphorylation | Uniprot | |
| S548 | Phosphorylation | Uniprot | |
| K553 | Ubiquitination | Uniprot | |
| S623 | Phosphorylation | Uniprot | |
| K628 | Ubiquitination | Uniprot | |
| K632 | Ubiquitination | Uniprot | |
| T653 | Phosphorylation | Uniprot | |
| S657 | Phosphorylation | Uniprot | |
| S787 | Phosphorylation | Uniprot | |
| S794 | Phosphorylation | Uniprot |
Research Backgrounds
Implicated in chromosome condensation and DNA damage induced cellular responses. May play a role in neurogenesis and regulation of the size of the cerebral cortex.
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Expressed in fetal brain, liver and kidney.
Interacts with CDC27 and maybe other components of the APC/C complex. Interacts with histone variant H2AX under DNA damage conditions.
BRCT domain 1 is required to prevent abnormal chromosome condensation. It binds directly to the SWI-SNF chromatin remodeling complex (PubMed:19925808).
BRCT domains 2 and 3 recognize phosphoserine/phosphothreonine marks on proteins with high selectivity, and mediate interaction with phosphorylated CDC27. They also mediate the dual recognition of phosphoserine and phosphotyrosine in the C-terminal tail of histone H2AX (PubMed:22139841, PubMed:22908299 and PubMed:22908299).
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