FAM3D Antibody - #DF4832
(4)
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Rat
Prediction:
Pig(89%), Rabbit(89%)
Clonality:
Polyclonal
Specificity:
FAM3D Antibody detects endogenous levels of total FAM3D.
RRID:
AB_2837197
Cite Format: Affinity Biosciences Cat# DF4832, RRID:AB_2837197.
Cite Format: Affinity Biosciences Cat# DF4832, RRID:AB_2837197.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
Cytokine-like protein EF-7; EF7; OIT1; Protein FAM3D precursor; UNQ567/PRO1130;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Sequence:
- Q96BQ1 FAM3D_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MRVSGVLRLLALIFAIVTTWMFIRSYMSFSMKTIRLPRWLAASPTKEIQVKKYKCGLIKPCPANYFAFKICSGAANVVGPTMCFEDRMIMSPVKNNVGRGLNIALVNGTTGAVLGQKAFDMYSGDVMHLVKFLKEIPGGALVLVASYDDPGTKMNDESRKLFSDLGSSYAKQLGFRDSWVFIGAKDLRGKSPFEQFLKNSPDTNKYEGWPELLEMEGCMPPKPF
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Rabbit
89
Pig
89
Horse
78
Bovine
78
Sheep
78
Dog
78
Xenopus
56
Chicken
56
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q96BQ1 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S43 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K52 | Ubiquitination | Uniprot | |
| K54 | Ubiquitination | Uniprot | |
| Y65 | Phosphorylation | Uniprot | |
| N107 | N-Glycosylation | Uniprot | |
| Y122 | Phosphorylation | Uniprot | |
| S163 | Phosphorylation | Uniprot | |
| Y169 | Phosphorylation | Uniprot |
Research Backgrounds
Subcellular Location:
Secreted.
Tissue Specificity:
Abundantly expressed in placenta and weakly expressed in small intestine.
Family&Domains:
Belongs to the FAM3 family.
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For Research Use Only.
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