EMR2 Antibody - #DF4875
| Product: | EMR2 Antibody |
| Catalog: | DF4875 |
| Description: | Rabbit polyclonal antibody to EMR2 |
| Application: | WB IHC IF/ICC |
| Reactivity: | Human |
| Mol.Wt.: | 85 KD; 90kD(Calculated). |
| Uniprot: | Q9UHX3 |
| RRID: | AB_2837238 |
Related Downloads
Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# DF4875, RRID:AB_2837238.
Fold/Unfold
CD 312; CD 312 antigen; CD312; CD312 antigen; Egf like module containing mucin like hormone receptor like 2; Egf like module containing mucin like hormone receptor like sequence 2; EGF like module EMR2; EGF-like module receptor 2; EGF-like module-containing mucin-like hormone receptor-like 2; EMR2; EMR2_HUMAN;
Immunogens
Expression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested. High expression in mast cells (PubMed:26841242).
- Q9UHX3 AGRE2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MGGRVFLVFLAFCVWLTLPGAETQDSRGCARWCPQDSSCVNATACRCNPGFSSFSEIITTPMETCDDINECATLSKVSCGKFSDCWNTEGSYDCVCSPGYEPVSGAKTFKNESENTCQDVDECQQNPRLCKSYGTCVNTLGSYTCQCLPGFKLKPEDPKLCTDVNECTSGQNPCHSSTHCLNNVGSYQCRCRPGWQPIPGSPNGPNNTVCEDVDECSSGQHQCDSSTVCFNTVGSYSCRCRPGWKPRHGIPNNQKDTVCEDMTFSTWTPPPGVHSQTLSRFFDKVQDLGRDYKPGLANNTIQSILQALDELLEAPGDLETLPRLQQHCVASHLLDGLEDVLRGLSKNLSNGLLNFSYPAGTELSLEVQKQVDRSVTLRQNQAVMQLDWNQAQKSGDPGPSVVGLVSIPGMGKLLAEAPLVLEPEKQMLLHETHQGLLQDGSPILLSDVISAFLSNNDTQNLSSPVTFTFSHRSVIPRQKVLCVFWEHGQNGCGHWATTGCSTIGTRDTSTICRCTHLSSFAVLMAHYDVQEEDPVLTVITYMGLSVSLLCLLLAALTFLLCKAIQNTSTSLHLQLSLCLFLAHLLFLVAIDQTGHKVLCSIIAGTLHYLYLATLTWMLLEALYLFLTARNLTVVNYSSINRFMKKLMFPVGYGVPAVTVAISAASRPHLYGTPSRCWLQPEKGFIWGFLGPVCAIFSVNLVLFLVTLWILKNRLSSLNSEVSTLRNTRMLAFKATAQLFILGCTWCLGILQVGPAARVMAYLFTIINSLQGVFIFLVYCLLSQQVREQYGKWSKGIRKLKTESEMHTLSSSAKADTSKPSTVN
PTMs - Q9UHX3 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K284 | Ubiquitination | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| Y670 | Phosphorylation | Uniprot | |
| T672 | Phosphorylation | Uniprot | |
| S674 | Phosphorylation | Uniprot | |
| T807 | Phosphorylation | Uniprot | |
| S809 | Phosphorylation | Uniprot | |
| S811 | Phosphorylation | Uniprot | |
| K813 | Ubiquitination | Uniprot | |
| K818 | Ubiquitination | Uniprot |
Research Backgrounds
Cell surface receptor that binds to the chondroitin sulfate moiety of glycosaminoglycan chains and promotes cell attachment. Promotes granulocyte chemotaxis, degranulation and adhesion. In macrophages, promotes the release of inflammatory cytokines, including IL8 and TNF. Signals probably through G-proteins. Is a regulator of mast cell degranulation.
Autoproteolytically cleaved into 2 subunits, an extracellular alpha subunit and a seven-transmembrane beta subunit.
Cell membrane>Multi-pass membrane protein. Cell projection>Ruffle membrane>Multi-pass membrane protein.
Note: Localized at the leading edge of migrating cells.
Expression is restricted to myeloid cells. Highest expression was found in peripheral blood leukocytes, followed by spleen and lymph nodes, with intermediate to low levels in thymus, bone marrow, fetal liver, placenta, and lung, and no expression in heart, brain, skeletal muscle, kidney, or pancreas. Expression is also detected in monocyte/macrophage and Jurkat cell lines but not in other cell lines tested. High expression in mast cells.
Forms a heterodimer, consisting of a large extracellular region non-covalently linked to a seven-transmembrane moiety. Interacts with chondroitin sulfate; the interaction with chondroitin sulfate is calcium-dependent. Interacts with CD55.
The GPS domain is necessary, but not sufficient for receptor cleavage, which require the entire extracellular stalk.
Binding to chondroitin sulfate is mediated by the fourth EGF domain.
Belongs to the G-protein coupled receptor 2 family. Adhesion G-protein coupled receptor (ADGR) subfamily.
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