Product: RON Antibody
Catalog: BF0576
Description: Mouse monoclonal antibody to RON
Application: WB ELISA
Reactivity: Human
Mol.Wt.: 152kDa; 152kD(Calculated).
Uniprot: Q04912
RRID: AB_2833660

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Product Info

ELISA 1:10000, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1636]
RON antibody detects endogenous levels of total RON.
Cite Format: Affinity Biosciences Cat# BF0576, RRID:AB_2833660.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


c met related tyrosine kinase; CD136; CD136 antigen; CDw136; Macrophage stimulating 1 receptor (c met related tyrosine kinase); Macrophage stimulating 1 receptor; Macrophage stimulating protein receptor alpha chain; MACROPHAGE STIMULATING PROTEIN RECEPTOR; Macrophage stimulating protein receptor beta chain; Macrophage-Stimulating 1 Receptor (MST1R); Macrophage-stimulating protein receptor beta chain; MSP receptor; Mst1r; MST1R variant RON30; MST1R variant RON62; NPCA3; p185 RON; p185-Ron; Protein-tyrosine kinase 8; PTK 8; ptk8; PTK8 protein tyrosine kinase 8; Recepteur d’origine nantais (RON); RON; RON protein tyrosine kinase; RON variant E2E3; RON_HUMAN; Soluble RON variant 1; Soluble RON variant 2; Soluble RON variant 3; Soluble RON variant 4; Stem cell derived tyrosine kinase;



Purified recombinant fragment of human RON expressed in E. Coli.


Expressed in colon, skin, lung and bone marrow.

RON (MST1R): macrophage stimulating 1 receptor (c-met-related tyrosine kinase). RON is a receptor tyrosine kinase that is translated as a single polypeptide and then proteolytically cleaved to yield a mature heterodimer consisting of an extracellular 35 kDa α chain disulfide-linked to a membrane-spanning 150 kDa beta chain.

PTMs - Q04912 As Substrate

Site PTM Type Enzyme
S215 Phosphorylation
K222 Ubiquitination
S226 Phosphorylation
S236 Phosphorylation
N488 N-Glycosylation
Y1012 Phosphorylation
Y1017 Phosphorylation
S1056 Phosphorylation
K1071 Ubiquitination
Y1101 Phosphorylation
Y1198 Phosphorylation
K1223 Ubiquitination
Y1238 Phosphorylation Q04912 (MST1R) , K9JYP4 (RON) , P08581 (MET)
Y1239 Phosphorylation P08581 (MET) , K9JYP4 (RON) , Q04912 (MST1R)
S1240 Phosphorylation
Y1317 Phosphorylation
Y1353 Phosphorylation Q04912 (MST1R) , P08581 (MET) , K9JYP4 (RON)
Y1360 Phosphorylation P08581 (MET) , K9JYP4 (RON) , Q04912 (MST1R)
S1394 Phosphorylation P31749 (AKT1)

PTMs - Q04912 As Enzyme

Substrate Site Source
P08581 (MET) Y1234 Uniprot
P08581 (MET) Y1235 Uniprot
P08581 (MET) Y1349 Uniprot
P08581 (MET) Y1356 Uniprot
Q04912 (MST1R) Y1238 Uniprot
Q04912 (MST1R) Y1239 Uniprot
Q04912 (MST1R) Y1353 Uniprot
Q04912 (MST1R) Y1360 Uniprot

Research Backgrounds


Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding to MST1 ligand. Regulates many physiological processes including cell survival, migration and differentiation. Ligand binding at the cell surface induces autophosphorylation of RON on its intracellular domain that provides docking sites for downstream signaling molecules. Following activation by ligand, interacts with the PI3-kinase subunit PIK3R1, PLCG1 or the adapter GAB1. Recruitment of these downstream effectors by RON leads to the activation of several signaling cascades including the RAS-ERK, PI3 kinase-AKT, or PLCgamma-PKC. RON signaling activates the wound healing response by promoting epithelial cell migration, proliferation as well as survival at the wound site. Plays also a role in the innate immune response by regulating the migration and phagocytic activity of macrophages. Alternatively, RON can also promote signals such as cell migration and proliferation in response to growth factors other than MST1 ligand.


Proteolytic processing yields the two subunits.

Autophosphorylated in response to ligand binding on Tyr-1238 and Tyr-1239 in the kinase domain leading to further phosphorylation of Tyr-1353 and Tyr-1360 in the C-terminal multifunctional docking site.

Ubiquitinated. Ubiquitination by CBL regulates the receptor stability and activity through proteasomal degradation.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in colon, skin, lung and bone marrow.

Subunit Structure:

Heterodimer of an alpha chain and a beta chain which are disulfide linked. Binds PLXNB1. Associates with and is negatively regulated by HYAL2. Interacts when phosphorylated with downstream effectors including PIK3R1, PCLG1, GRB2 and GAB1. Interacts with integrin beta1/ITGB1 in a ligand-independent fashion.


Belongs to the protein kinase superfamily. Tyr protein kinase family.

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