Product: GPR175 Antibody
Catalog: DF4903
Description: Rabbit polyclonal antibody to GPR175
Application: WB IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish, Bovine, Horse, Rabbit, Dog, Chicken
Mol.Wt.: 41 KD; 41kD(Calculated).
Uniprot: Q86W33
RRID: AB_2837256

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 100ul $280 In stock
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Lead Time: Same day delivery

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(80%), Bovine(100%), Horse(100%), Rabbit(100%), Dog(100%), Chicken(87%)
Clonality:
Polyclonal
Specificity:
GPR175 Antibody detects endogenous levels of total GPR175.
RRID:
AB_2837256
Cite Format: Affinity Biosciences Cat# DF4903, RRID:AB_2837256.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

FLJ32197; G protein coupled receptor 175; GPR175; Integral membrane protein GPR175; PP6566; Seven transmembrane domain orphan receptor; TMEM227; Tpra1; TPRA1_HUMAN; TPRA40; Transmembrane domain protein regulated in adipocytes; Transmembrane domain protein regulated in adipocytes 40 kDa; Transmembrane protein 227; Transmembrane protein adipocyte-associated 1;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q86W33 TPRA1_HUMAN:

Ubiquitous, with higher levels in heart, placenta and kidney.

Sequence:
MDTLEEVTWANGSTALPPPLAPNISVPHRCLLLLYEDIGTSRVRYWDLLLLIPNVLFLIFLLWKLPSARAKIRITSSPIFITFYILVFVVALVGIARAVVSMTVSTSNAATVADKILWEITRFFLLAIELSVIILGLAFGHLESKSSIKRVLAITTVLSLAYSVTQGTLEILYPDAHLSAEDFNIYGHGGRQFWLVSSCFFFLVYSLVVILPKTPLKERISLPSRRSFYVYAGILALLNLLQGLGSVLLCFDIIEGLCCVDATTFLYFSFFAPLIYVAFLRGFFGSEPKILFSYKCQVDETEEPDVHLPQPYAVARREGLEAAGAAGASAASYSSTQFDSAGGVAYLDDIASMPCHTGSINSTDSERWKAINA

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Dog
100
Rabbit
100
Chicken
87
Zebrafish
80
Pig
0
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86W33 As Substrate

Site PTM Type Enzyme
T3 Phosphorylation
T14 Phosphorylation
K289 Ubiquitination
S293 Phosphorylation
Y312 Phosphorylation

Research Backgrounds

Subcellular Location:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Ubiquitous, with higher levels in heart, placenta and kidney.

Family&Domains:

Belongs to the UPF0359 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.