GPR149 Antibody | Affinity Biosciences

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GPR149 Antibody - Western blot analysis of extracts from P19 cells, using GPR149 Antibody.

Product:	GPR149 Antibody
Catalog:	DF4952
Description:	Rabbit polyclonal antibody to GPR149
Application:	WB IF/ICC
Reactivity:	Human, Mouse
Prediction:	Rabbit
Mol.Wt.:	81 KD; 81kD(Calculated).
Uniprot:	Q86SP6
RRID:	AB_2837305

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Rabbit(83%)

Clonality:

Polyclonal

Specificity:

GPR149 Antibody detects endogenous levels of total GPR149.

RRID:

AB_2837305
Cite Format: Affinity Biosciences Cat# DF4952, RRID:AB_2837305.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

G protein coupled receptor 149; G protein coupled receptor PGR10; GPR 149; GPR149; IEDA; PGR 10; PGR10; Probable G protein coupled receptor 149;

Immunogens

Immunogen:

Uniprot:

Q86SP6(GP149_HUMAN) >>Visit HPA database.

Gene(ID):

GPR149(344758)

Sequence:

Q86SP6 GP149_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSLFLSNLSTNDSSLWKENHNSTDLLNPPGTLNIYLFCLTCLMTFAALVGSIYSLISLLKMQNRTVVSMLVASWSVDDLMSVLSVTIFMFLQWPNEVPGYFQFLCTTSALMYLCQGLSSNLKATLLVSYNFYTMHRGVGSQTASRRSGQVLGVVLTVWAASLLLSALPLCGWGAFVRTPWGCLVDCSSSYVLFLSIVYALAFGLLVGLSVPLTHRLLCSEEPPRLHSNYQEISRGASIPGTPPTAGRVVSLSPEDAPGPSLRRSGGCSPSSDTVFGPGAPAAAGAEACRRENRGTLYGTRSFTVSVAQKRFALILALTKVVLWLPMMMHMVVQNVVGFQSLPLETFSFLLTLLATTVTPVFVLSKRWTHLPCGCIINCRQNAYAVASDGKKIKRKGFEFNLSFQKSYGIYKIAHEDYYDDDENSIFYHNLMNSECETTKDPQRDNRNIFNAIKVEISTTPSLDSSTQRGINKCTNTDITEAKQDSNNKKDAFSDKTGGDINYEETTFSEGPERRLSHEESQKPDLSDWEWCRSKSERTPRQRSGYALAIPLCAFQGTVSLHAPTGKTLSLSTYEVSAEGQKITPASKKIEVYRSKSVGHEPNSEDSSSTFVDTSVKIHLEVLEICDNEEALDTVSIISNISQSSTQVRSPSLRYSRKENRFVSCDLGETASYSLFLPTSNPDGDINISIPDTVEAHRQNSKRQHQERDGYQEEIQLLNKAYRKREEESKGS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Rabbit

Pig

Horse

Bovine

Sheep

Dog

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q86SP6 As Substrate

Q86SP6

Site	PTM Type	Source
S250	Phosphorylation	Uniprot
S252	Phosphorylation	Uniprot
T295	Phosphorylation	Uniprot
T303	Phosphorylation	Uniprot
T318	Phosphorylation	Uniprot
T459	Phosphorylation	Uniprot
S543	Phosphorylation	Uniprot
S559	Phosphorylation	Uniprot

Research Backgrounds

Q86SP6_GP149_HUMAN

Function:

Orphan receptor.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.

GPR149 Antibody - #DF4952