GPR158 Antibody | Affinity Biosciences

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GPR158 Antibody - Western blot analysis of extracts from COLO205 cells(heat-shock treatment), using GPR158 Antibody at 1/1000 dilution.

Product:	GPR158 Antibody
Catalog:	DF4957
Description:	Rabbit polyclonal antibody to GPR158
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse
Prediction:	Pig, Bovine, Horse, Sheep
Mol.Wt.:	150 KD; 135kD(Calculated).
Uniprot:	Q5T848
RRID:	AB_2837310

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

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Related Downloads

MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse

Prediction:

Pig(100%), Bovine(89%), Horse(89%), Sheep(100%)

Clonality:

Polyclonal

Specificity:

GPR158 Antibody detects endogenous levels of total GPR158.

RRID:

AB_2837310
Cite Format: Affinity Biosciences Cat# DF4957, RRID:AB_2837310.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

5330427M13Rik; GP158_HUMAN; GPR158; mKIAA1136; Probable G-protein coupled receptor 158; RP11-59G22.1; RP23-133B16.1;

Immunogens

Immunogen:

Uniprot:

Q5T848(GP158_HUMAN) >>Visit HPA database.

Gene(ID):

GPR158(57512)

Sequence:

Q5T848 GP158_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MGAMAYPLLLCLLLAQLGLGAVGASRDPQGRPDSPRERTPKGKPHAQQPGRASASDSSAPWSRSTDGTILAQKLAEEVPMDVASYLYTGDSHQLKRANCSGRYELAGLPGKWPALASAHPSLHRALDTLTHATNFLNVMLQSNKSREQNLQDDLDWYQALVWSLLEGEPSISRAAITFSTDSLSAPAPQVFLQATREESRILLQDLSSSAPHLANATLETEWFHGLRRKWRPHLHRRGPNQGPRGLGHSWRRKDGLGGDKSHFKWSPPYLECENGSYKPGWLVTLSSAIYGLQPNLVPEFRGVMKVDINLQKVDIDQCSSDGWFSGTHKCHLNNSECMPIKGLGFVLGAYECICKAGFYHPGVLPVNNFRRRGPDQHISGSTKDVSEEAYVCLPCREGCPFCADDSPCFVQEDKYLRLAIISFQALCMLLDFVSMLVVYHFRKAKSIRASGLILLETILFGSLLLYFPVVILYFEPSTFRCILLRWARLLGFATVYGTVTLKLHRVLKVFLSRTAQRIPYMTGGRVMRMLAVILLVVFWFLIGWTSSVCQNLEKQISLIGQGKTSDHLIFNMCLIDRWDYMTAVAEFLFLLWGVYLCYAVRTVPSAFHEPRYMAVAVHNELIISAIFHTIRFVLASRLQSDWMLMLYFAHTHLTVTVTIGLLLIPKFSHSSNNPRDDIATEAYEDELDMGRSGSYLNSSINSAWSEHSLDPEDIRDELKKLYAQLEIYKRKKMITNNPHLQKKRCSKKGLGRSIMRRITEIPETVSRQCSKEDKEGADHGTAKGTALIRKNPPESSGNTGKSKEETLKNRVFSLKKSHSTYDHVRDQTEESSSLPTESQEEETTENSTLESLSGKKLTQKLKEDSEAESTESVPLVCKSASAHNLSSEKKTGHPRTSMLQKSLSVIASAKEKTLGLAGKTQTAGVEERTKSQKPLPKDKETNRNHSNSDNTETKDPAPQNSNPAEEPRKPQKSGIMKQQRVNPTTANSDLNPGTTQMKDNFDIGEVCPWEVYDLTPGPVPSESKVQKHVSIVASEMEKNPTFSLKEKSHHKPKAAEVCQQSNQKRIDKAEVCLWESQGQSILEDEKLLISKTPVLPERAKEENGGQPRAANVCAGQSEELPPKAVASKTENENLNQIGHQEKKTSSSEENVRGSYNSSNNFQQPLTSRAEVCPWEFETPAQPNAGRSVALPASSALSANKIAGPRKEEIWDSFKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Sheep

100

Horse

Bovine

Dog

Rabbit

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q5T848 As Substrate

Q5T848

Site	PTM Type	Source
S55	Phosphorylation	Uniprot
S57	Phosphorylation	Uniprot
S64	Phosphorylation	Uniprot
S142	Phosphorylation	Uniprot
S145	Phosphorylation	Uniprot
S249	Phosphorylation	Uniprot
Y612	Phosphorylation	Uniprot
K732	Methylation	Uniprot
S753	Phosphorylation	Uniprot
K774	Ubiquitination	Uniprot
T870	Phosphorylation	Uniprot
S872	Phosphorylation	Uniprot
S879	Phosphorylation	Uniprot
T913	Phosphorylation	Uniprot
T929	Phosphorylation	Uniprot
S931	Phosphorylation	Uniprot
S1193	Phosphorylation	Uniprot
S1194	Phosphorylation	Uniprot
S1197	Phosphorylation	Uniprot

Research Backgrounds

Q5T848_GP158_HUMAN

Function:

Orphan receptor.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 3 family.

Restrictive clause

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For Research Use Only.
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GPR158 Antibody - #DF4957