OR2K2 Antibody | Affinity Biosciences

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IF/ICC
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OR2K2 Antibody - Western blot analysis of extracts from HT-29 cells, using OR2K2 antibody.

OR2K2 Antibody - DF5075 staining Hela cells by IF/ICC.

Product:	OR2K2 Antibody
Catalog:	DF5075
Description:	Rabbit polyclonal antibody to OR2K2
Application:	WB IF/ICC
Reactivity:	Human
Mol.Wt.:	35 KD; 35kD(Calculated).
Uniprot:	Q8NGT1
RRID:	AB_2837434

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

Size	Price	Inventory
100ul	$280	In stock
200ul	$350	In stock

Lead Time: Same day delivery

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Related Downloads

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human

Clonality:

Polyclonal

Specificity:

OR2K2 Antibody detects endogenous levels of total OR2K2.

RRID:

AB_2837434
Cite Format: Affinity Biosciences Cat# DF5075, RRID:AB_2837434.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

HSHTPCRH06; HTPCRH06; MGC133152; Olfactory receptor 2K2; Olfactory receptor OR9-17; Olfactory receptor, family 2, subfamily AN, member 1 pseudogene; Olfactory receptor, family 2, subfamily AR, member 1 pseudogene; Olfactory receptor, family 2, subfamily K, member 2; OR2AN1P; OR2AR1P; OR2K2; OR2K2_HUMAN;

Immunogens

Immunogen:

Uniprot:

Q8NGT1(OR2K2_HUMAN) >>Visit HPA database.

Gene(ID):

OR2K2(26248)

Sequence:

Q8NGT1 OR2K2_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MQGENFTIWSIFFLEGFSQYPGLEVVLFVFSLVMYLTTLLGNSTLILITILDSRLKTPMYLFLGNLSFMDICYTSASVPTLLVNLLSSQKTIIFSGCAVQMYLSLAMGSTECVLLAVMAYDRYVAICNPLRYSIIMNRCVCARMATVSWVTGCLTALLETSFALQIPLCGNLIDHFTCEILAVLKLACTSSLLMNTIMLVVSILLLPIPMLLVCISYIFILSTILRITSAEGRNKAFSTCGAHLTVVILYYGAALSMYLKPSSSNAQKIDKIISLLYGVLTPMLNPIIYSLRNKEVKDAMKKLLGKITLHQTHEHL

PTMs - Q8NGT1 As Substrate

Q8NGT1

Site	PTM Type	Enzyme	Source
T177	Phosphorylation		Uniprot

Research Backgrounds

Q8NGT1_OR2K2_HUMAN

Function:

Odorant receptor.

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Family&Domains:

Belongs to the G-protein coupled receptor 1 family.

Research Fields

· Organismal Systems > Sensory system > Olfactory transduction.

References

1). Epigenetic silencing of OR and TAS2R genes expression in human orbitofrontal cortex at early stages of sporadic Alzheimer’s disease. Cellular and Molecular Life Sciences, 2023 (PubMed: 37405535) [IF=6.2]

2). Olfactory Receptor OR2K2 Expression in Human Choroid Plexus as a Potential Marker in Early Sporadic Alzheimer's Disease. Genes, 2024 (PubMed: 38540444) [IF=3.5]

Application: WB Species: Human Sample: CP tissue

Figure 1. OR2K2 expression in CP. (a,b) Absolute measurement of OR (a) and TASR (b) gene transcription with selfie-dPCR. (c–e) OR2K2 relative protein expression in choroid plexus. Representative Western blot and relative quantification using C-terminal (c) and N-terminal (d) OR2K2 antibodies. Full immunoblots are presented in Figure S2. * p < 0.05; ** p < 0.01; *** p < 0.001, One-way ANOVA followed by Bonferroni’s multiple comparison test. Ns, not significant. (e) Representative confocal microscopic images of choroid plexus epithelial cells demonstrating the expressions of OR2K2 (red) and nuclear stain DAPI (blue) in control subject (80-year-old male) and patients with AD (76-year-old male, AD Braak I, and 82-year-old male, AD Braak V). For the negative control, the choroid plexus section was incubated without primary antibody. Scale bar, 100 μm. In the lower panel, a magnification of the region is indicated in the white boxes in the upper panel. Scale bar, 20 μm. AD, Alzheimer’s disease.

Restrictive clause

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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OR2K2 Antibody - #DF5075