Product: PEVR1 Antibody
Catalog: DF5141
Description: Rabbit polyclonal antibody to PEVR1
Application: WB IF/ICC
Reactivity: Human
Prediction: Pig, Horse, Sheep, Dog
Mol.Wt.: 46 KD; 46kD(Calculated).
Uniprot: Q9HAB3
RRID: AB_2837500

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:1000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human
Prediction:
Pig(88%), Horse(88%), Sheep(88%), Dog(88%)
Clonality:
Polyclonal
Specificity:
PEVR1 Antibody detects endogenous levels of total PEVR1.
RRID:
AB_2837500
Cite Format: Affinity Biosciences Cat# DF5141, RRID:AB_2837500.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

GPR172A; hRFT3; PAR1; PERV-A receptor 1; Porcine endogenous retrovirus A receptor 1; Protein GPR172A; RFT3; RFT3_HUMAN; Riboflavin transporter 3;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
Q9HAB3 S52A2_HUMAN:

Highly expressed in brain, fetal brain and salivary gland. Weakly expressed in other tissues.

Sequence:
MAAPTPARPVLTHLLVALFGMGSWAAVNGIWVELPVVVKELPEGWSLPSYVSVLVALGNLGLLVVTLWRRLAPGKDEQVPIRVVQVLGMVGTALLASLWHHVAPVAGQLHSVAFLALAFVLALACCASNVTFLPFLSHLPPRFLRSFFLGQGLSALLPCVLALVQGVGRLECPPAPINGTPGPPLDFLERFPASTFFWALTALLVASAAAFQGLLLLLPPPPSVPTGELGSGLQVGAPGAEEEVEESSPLQEPPSQAAGTTPGPDPKAYQLLSARSACLLGLLAATNALTNGVLPAVQSFSCLPYGRLAYHLAVVLGSAANPLACFLAMGVLCRSLAGLGGLSLLGVFCGGYLMALAVLSPCPPLVGTSAGVVLVVLSWVLCLGVFSYVKVAASSLLHGGGRPALLAAGVAIQVGSLLGAVAMFPPTSIYHVFHSRKDCADPCDS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
88
Sheep
88
Dog
88
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9HAB3 As Substrate

Site PTM Type Enzyme
K75 Ubiquitination
K267 Sumoylation
S273 Phosphorylation
Y305 Phosphorylation

Research Backgrounds

Function:

Plasma membrane transporter mediating the uptake by cells of the water soluble vitamin B2/riboflavin that plays a key role in biochemical oxidation-reduction reactions of the carbohydrate, lipid, and amino acid metabolism. Humans are unable to synthesize vitamin B2/riboflavin and must obtain it via intestinal absorption. May also act as a receptor for 4-hydroxybutyrate (Probable).

(Microbial infection) In case of infection by retroviruses, acts as a cell receptor to retroviral envelopes similar to the porcine endogenous retrovirus (PERV-A).

Subcellular Location:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in brain, fetal brain and salivary gland. Weakly expressed in other tissues.

Family&Domains:

Belongs to the riboflavin transporter family.

Restrictive clause

 

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