Cytokeratin 19 Antibody - #AF5106

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Product: Cytokeratin 19 Antibody
Catalog: AF5106
Description: Rabbit polyclonal antibody to Cytokeratin 19
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Bovine, Rabbit, Dog
Mol.Wt.: 44 kDa; 44kD(Calculated).
Uniprot: P08727
RRID: AB_2837592

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(90%), Rabbit(90%), Dog(82%)
Clonality:
Polyclonal
Specificity:
Cytokeratin 19 Antibody detects endogenous levels of total Cytokeratin 19.
RRID:
AB_2837592
Cite Format: Affinity Biosciences Cat# AF5106, RRID:AB_2837592.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

40 kDa keratin intermediate filament; CK 19; CK-19; CK19; Cytokeratin 19; Cytokeratin-19; K19; K1C19_HUMAN; K1CS; Keratin 19; Keratin type I 40 kD; Keratin type I 40kD; Keratin type I cytoskeletal 19; Keratin, type I cytoskeletal 19; Keratin, type I, 40 kd; Keratin-19; KRT19; MGC15366;

Immunogens

Immunogen:

A synthesized peptide derived from human Cytokeratin 19, corresponding to a region within C-terminal amino acids.

Uniprot:

P08727(K1C19_HUMAN) >>Visit HPA database.

Gene(ID):
KRT19(3880)
Expression:
P08727 K1C19_HUMAN:

Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Description:
Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin
Sequence:
MTSYSYRQSSATSSFGGLGGGSVRFGPGVAFRAPSIHGGSGGRGVSVSSARFVSSSSSGAYGGGYGGVLTASDGLLAGNEKLTMQNLNDRLASYLDKVRALEAANGELEVKIRDWYQKQGPGPSRDYSHYYTTIQDLRDKILGATIENSRIVLQIDNARLAADDFRTKFETEQALRMSVEADINGLRRVLDELTLARTDLEMQIEGLKEELAYLKKNHEEEISTLRGQVGGQVSVEVDSAPGTDLAKILSDMRSQYEVMAEQNRKDAEAWFTSRTEELNREVAGHTEQLQMSRSEVTDLRRTLQGLEIELQSQLSMKAALEDTLAETEARFGAQLAHIQALISGIEAQLGDVRADSERQNQEYQRLMDIKSRLEQEIATYRSLLEGQEDHYNNLSASKVL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
90
Bovine
90
Dog
82
Xenopus
70
Chicken
64
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Involved in the organization of myofibers. Together with KRT8, helps to link the contractile apparatus to dystrophin at the costameres of striated muscle.

Tissue Specificity:

Expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. Also observed in sweat gland and mammary gland ductal and secretory cells, bile ducts, gastrointestinal tract, bladder urothelium, oral epithelia, esophagus, ectocervical epithelium (at protein level). Expressed in epidermal basal cells, in nipple epidermis and a defined region of the hair follicle. Also seen in a subset of vascular wall cells in both the veins and artery of human umbilical cord, and in umbilical cord vascular smooth muscle. Observed in muscle fibers accumulating in the costameres of myoplasm at the sarcolemma in structures that contain dystrophin and spectrin.

Family&Domains:

This keratin differs from all other IF proteins in lacking the C-terminal tail domain.

Belongs to the intermediate filament family.

Research Fields

· Organismal Systems > Endocrine system > Estrogen signaling pathway.   (View pathway)

References

1). 3D-printed scaffold harboring copper ions combined with near-infrared irradiation for local therapy of cancer. iScience, 2023 (PubMed: 37860764) [IF=5.8]

Application: IF/ICC    Species: Human    Sample:

Figure 4 Immunofluorescence staining of CK19, gal-3, and Ki-67 in the primary tumor tissues and the paired organoids The passage number for each patient’s organoid line was: patient 1, P3; patient 2, P2; patient 3, P4; patient 4, P5; patient 5, P4; patient 6, P3. T, tumor; O, organoids; gal-3, galectin-3. Scale bar, 100 μm.

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