Connexin 32 Antibody - #AF5132

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Product: Connexin 32 Antibody
Catalog: AF5132
Description: Rabbit polyclonal antibody to Connexin 32
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: P08034
RRID: AB_2837618

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Connexin 32 Antibody detects endogenous levels of total Connexin 32.
RRID:
AB_2837618
Cite Format: Affinity Biosciences Cat# AF5132, RRID:AB_2837618.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Charcot Marie Tooth neuropathy X linked; CMTX 1; CMTX; CMTX1; Connexin-32; Connexin32; CX 32; Cx32; CXB1_HUMAN; GAP junction 28 kDa liver protein; Gap junction beta 1 protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; Gap junction protein beta 1; GJB 1; GJB1;

Immunogens

Immunogen:

A synthesized peptide derived from human Connexin 32, corresponding to a region within the internal amino acids.

Uniprot:

P08034(CXB1_HUMAN) >>Visit HPA database.

Gene(ID):
GJB1(2705)
Description:
A form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology
Sequence:
MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDQFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
90
Chicken
80
Xenopus
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the connexin family. Beta-type (group I) subfamily.

References

1). Identification of proteomic markers of chronic prostatitis/chronic pelvic pain syndrome treated with melatonin using a tandem mass tag approach. Current urology, 2025 (PubMed: 40894284) [IF=0.9]

Application: WB    Species: Mouse    Sample:

Figure 6 In the EAP model, Ckmt1 expression was reduced, whereas Gjb1 expression was increased after the treatment of melatonin. (A) Two selected proteins in the mouse prostate tissue from the control, EAP, and EAP-Mel groups were verified by Western blotting. The abundance of ckmt1 and Gjb1 proteins in the 3 groups was analyzed by Western blotting. GAPDH was used as an internal control. (B) Ckmt1 and Gjb1 bands were analyzed with the relative density of the corresponding GAPDH bands, respectively. ***p < 0.001, **p < 0.01, *p < 0.05. EAP = experimental autoimmune prostatitis. The Western blot image displayed is a cropped version, focusing on the regions containing the target proteins (Ckmt1, Gjb1) and the reference protein (GAPDH). The cropping was confined to the vicinity of their respective molecular weights (45 kDa for Ckmt1, 32 kDa for Gjb1, and 42 kDa for GAPDH) without any alteration or splicing of the bands. The complete original blot is retained and can be provided upon request for verification.

Restrictive clause

 

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For Research Use Only.
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