Connexin 32 Antibody - #AF5132

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Product: Connexin 32 Antibody
Catalog: AF5132
Description: Rabbit polyclonal antibody to Connexin 32
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 32 kDa; 32kD(Calculated).
Uniprot: P08034
RRID: AB_2837618

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(90%), Dog(100%), Chicken(80%)
Clonality:
Polyclonal
Specificity:
Connexin 32 Antibody detects endogenous levels of total Connexin 32.
RRID:
AB_2837618
Cite Format: Affinity Biosciences Cat# AF5132, RRID:AB_2837618.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Charcot Marie Tooth neuropathy X linked; CMTX 1; CMTX; CMTX1; Connexin-32; Connexin32; CX 32; Cx32; CXB1_HUMAN; GAP junction 28 kDa liver protein; Gap junction beta 1 protein; Gap junction beta-1 protein; Gap junction protein beta 1 32kD; Gap junction protein beta 1; GJB 1; GJB1;

Immunogens

Immunogen:
Uniprot:

P08034(CXB1_HUMAN) >>Visit HPA database.

Gene(ID):
GJB1(2705)
Description:
A form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. Charcot-Marie-Tooth disease is classified in two main groups on the basis of electrophysiologic properties and histopathology
Sequence:
MNWTGLYTLLSGVNRHSTAIGRVWLSVIFIFRIMVLVVAAESVWGDEKSSFICNTLQPGCNSVCYDQFFPISHVRLWSLQLILVSTPALLVAMHVAHQQHIEKKMLRLEGHGDPLHLEEVKRHKVHISGTLWWTYVISVVFRLLFEAVFMYVFYLLYPGYAMVRLVKCDVYPCPNTVDCFVSRPTEKTVFTVFMLAASGICIILNVAEVVYLIIRACARRAQRRSNPPSRKGSGFGHRLSPEYKQNEINKLLSEQDGSLKDILRRSPGTGAGLAEKSDRCSAC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
90
Chicken
80
Xenopus
78
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P08034 As Substrate

Site PTM Type Enzyme
S229 Phosphorylation P17252 (PRKCA)
S233 Phosphorylation P17612 (PRKACA) , P17252 (PRKCA)
S240 Phosphorylation
Y243 Phosphorylation
K250 Ubiquitination
S258 Phosphorylation
K260 Ubiquitination
S266 Phosphorylation
T269 Phosphorylation
K276 Ubiquitination
S277 Phosphorylation
S281 Phosphorylation

Research Backgrounds

Function:

One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell.

Subcellular Location:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

A connexon is composed of a hexamer of connexins. Interacts with CNST (By similarity).

Family&Domains:

Belongs to the connexin family. Beta-type (group I) subfamily.

Restrictive clause

 

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