Product: AXL Antibody
Catalog: BF0290
Description: Mouse monoclonal antibody to AXL
Application: WB IF/ICC ELISA
Reactivity: Human
Mol.Wt.: 97.4kDa; 98kD(Calculated).
Uniprot: P30530
RRID: AB_2833702

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Product Info

ELISA 1:10000, WB 1:500-1:2000, IF/ICC 1:200-1:1000
*The optimal dilutions should be determined by the end user.

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Monoclonal [AFB1678]
AXL antibody detects endogenous levels of total AXL.
Cite Format: Affinity Biosciences Cat# BF0290, RRID:AB_2833702.
Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.


Adhesion related kinase; AI323647; Ark; Axl; AXL oncogene; AXL receptor tyrosine kinase; AXL transforming gene; AXL transforming sequence/gene; EC; JTK11; Oncogene AXL; Tyro7; Tyrosine protein kinase receptor UFO; Tyrosine-protein kinase receptor UFO; UFO; UFO_HUMAN;



Purified recombinant fragment of human AXL expressed in E. Coli.


Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

The protein encoded by this gene is a member of the receptor tyrosine kinase subfamily. Although it is similar to other receptor tyrosine kinases, the Axl protein represents a unique structure of the extracellular region that juxtaposes IgL and FNIII repeats. It transduce signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6. It is involved in the stimulation of cell proliferation. This receptor can also mediate cell aggregation by homophilic binding. Axl is a chronic myelogenous leukemia-associated oncogene and also associated with colon cancer and melanoma. It is in close vicinity to the bcl3 oncogene which is at 19q13.1-q13.2. The Axl gene is evolutionarily conserved between vertebrate species. This gene has two different alternatively spliced transcript variants.

PTMs - P30530 As Substrate

Site PTM Type Enzyme
S100 Phosphorylation
T200 Phosphorylation
S201 Phosphorylation
S202 Phosphorylation
S204 Phosphorylation
T303 Phosphorylation
Y481 Phosphorylation
S503 Phosphorylation
S515 Phosphorylation
K523 Ubiquitination
K525 Ubiquitination
K535 Ubiquitination
K540 Ubiquitination
K563 Ubiquitination
K570 Ubiquitination
S612 Phosphorylation
Y634 Phosphorylation
Y643 Phosphorylation
K666 Ubiquitination
S694 Phosphorylation
K696 Ubiquitination
Y698 Phosphorylation
Y702 Phosphorylation
Y703 Phosphorylation
K710 Ubiquitination
K714 Ubiquitination
R724 Methylation
Y726 Phosphorylation
Y759 Phosphorylation
Y772 Phosphorylation
Y779 Phosphorylation P30530 (AXL) , P00533 (EGFR)
Y814 Phosphorylation
Y821 Phosphorylation P30530 (AXL)
S853 Phosphorylation
Y866 Phosphorylation P30530 (AXL)
T873 Phosphorylation
S875 Phosphorylation
S884 Phosphorylation

PTMs - P30530 As Enzyme

Substrate Site Source
P30530-1 (AXL) Y779 Uniprot
P30530-1 (AXL) Y821 Uniprot
P30530 (AXL) Y866 Uniprot

Research Backgrounds


Receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factor GAS6 and which is thus regulating many physiological processes including cell survival, cell proliferation, migration and differentiation. Ligand binding at the cell surface induces dimerization and autophosphorylation of AXL. Following activation by ligand, ALX binds and induces tyrosine phosphorylation of PI3-kinase subunits PIK3R1, PIK3R2 and PIK3R3; but also GRB2, PLCG1, LCK and PTPN11. Other downstream substrate candidates for AXL are CBL, NCK2, SOCS1 and TNS2. Recruitment of GRB2 and phosphatidylinositol 3 kinase regulatory subunits by AXL leads to the downstream activation of the AKT kinase. GAS6/AXL signaling plays a role in various processes such as endothelial cell survival during acidification by preventing apoptosis, optimal cytokine signaling during human natural killer cell development, hepatic regeneration, gonadotropin-releasing hormone neuron survival and migration, platelet activation, or regulation of thrombotic responses. Plays also an important role in inhibition of Toll-like receptors (TLRs)-mediated innate immune response.

(Microbial infection) Acts as a receptor for lassa virus and lymphocytic choriomeningitis virus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.

(Microbial infection) Acts as a receptor for Ebolavirus, possibly through GAS6 binding to phosphatidyl-serine at the surface of virion envelope.


Monoubiquitinated upon GAS6-binding. A very small proportion of the receptor could be subjected to polyubiquitination in a very transient fashion.

Phosphorylated at tyrosine residues by autocatalysis, which activates kinase activity.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Highly expressed in metastatic colon tumors. Expressed in primary colon tumors. Weakly expressed in normal colon tissue.

Subunit Structure:

Heterodimer and heterotetramer with ligand GAS6. Interacts with CBL, GRB2, LCK, NCK2, PIK3R1, PIK3R2, PIK3R3, PLCG1, SOCS1 and TNS2. Part of a complex including AXL, TNK2 and GRB2, in which GRB2 promotes AXL recruitment by TNK2.


Belongs to the protein kinase superfamily. Tyr protein kinase family. AXL/UFO subfamily.

Research Fields

· Human Diseases > Drug resistance: Antineoplastic > EGFR tyrosine kinase inhibitor resistance.

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