SOX2 Antibody - #AF5140

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Product: SOX2 Antibody
Catalog: AF5140
Description: Rabbit polyclonal antibody to SOX2
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 34 kDa; 34kD(Calculated).
Uniprot: P48431
RRID: AB_2837626

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(100%)
Clonality:
Polyclonal
Specificity:
SOX2 Antibody detects endogenous levels of total SOX2.
RRID:
AB_2837626
Cite Format: Affinity Biosciences Cat# AF5140, RRID:AB_2837626.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ANOP3; cb236; Delta EF2a; lcc; MCOPS3; MGC148683; MGC2413; RGD1565646; Sex determining region Y box 2; SOX 2; Sox2; SOX2_HUMAN; SRY (sex determining region Y) box 2; SRY box containing gene 2; SRY related HMG box 2; SRY related HMG box gene 2; SRY-box 2; Transcription factor SOX 2; Transcription factor SOX-2; ysb;

Immunogens

Immunogen:
Uniprot:

P48431(SOX2_HUMAN) >>Visit HPA database.

Gene(ID):
SOX2(6657)
Description:
Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
Sequence:
MYNMMETELKPPGPQQTSGGGGGNSTAAAAGGNQKNSPDRVKRPMNAFMVWSRGQRRKMAQENPKMHNSEISKRLGAEWKLLSETEKRPFIDEAKRLRALHMKEHPDYKYRPRRKTKTLMKKDKYTLPGGLLAPGGNSMASGVGVGAGLGAGVNQRMDSYAHMNGWSNGSYSMMQDQLGYPQHPGLNAHGAAQMQPMHRYDVSALQYNSMTSSQTYMNGSPTYSMSYSQQGTPGMALGSMGSVVKSEASSSPPVVTSSSHSRAPCQAGDLRDMISMYLPGAEVPEPAAPSRLHMSQHYQSGPVPGTAINGTLPLSHM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Chicken
100
Rabbit
100
Zebrafish
79
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P48431 As Substrate

Site PTM Type Enzyme
S37 Phosphorylation
K73 Acetylation
T116 Phosphorylation
T118 Phosphorylation P31749 (AKT1)
K121 Ubiquitination
Y125 Phosphorylation
T126 Phosphorylation
S138 Phosphorylation
S220 Phosphorylation
K245 Sumoylation
S246 Phosphorylation
S249 Phosphorylation
S250 Phosphorylation
S251 Phosphorylation

Research Backgrounds

Function:

Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Binds to the proximal enhancer region of NANOG (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency. Downstream SRRT target that mediates the promotion of neural stem cell self-renewal (By similarity). Keeps neural cells undifferentiated by counteracting the activity of proneural proteins and suppresses neuronal differentiation (By similarity). May function as a switch in neuronal development (By similarity).

PTMs:

Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with ZSCAN10 (By similarity). Interacts with SOX3 and FGFR1 (By similarity). Interacts with GLIS1. Interacts with POU5F1; binds synergistically with POU5F1 to DNA (By similarity).

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Signaling pathways regulating pluripotency of stem cells.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

References

1). MiR-301 regulates the SIRT1/SOX2 pathway via CPEB1 in the breast cancer progression. Molecular Therapy-Oncolytics (PubMed: 34377766) [IF=5.7]
2). CDC20 is a novel biomarker for improved clinical predictions in epithelial ovarian cancer. American Journal of Cancer Research (PubMed: 35968331) [IF=5.3]

Application: WB    Species: Human    Sample: ovarian cancer cell

Figure 4 A. qRT-PCR detection of CDC20 and SOX2 expression in ovarian cancer cell lines. B. Western blot detection of CDC20 and SOX2 expression in ovarian cancer cell lines. C. Proliferation ability of ovarian cancer cells after silencing CDC20. D. Quadrant of apoptosis in flow cytometry. E. Transwell assays to investigate migratory ablilities of ovarian cancer cell lines transfected with shRNA-NC or shRNA01, 02, 03. Sample size =4. F. qRT-PCR detection of E-cadherin, BAX, BCL-2, Cyclin D1 and Cyclin D2 expression in ovarian cancer cell lines. ****P<0.0001, ***P<0.001, *P<0.05, compared with shRNA-NC.
3). The adenosine-A2a receptor regulates the radioresistance of gastric cancer via PI3K-AKT-mTOR pathway. International Journal of Clinical Oncology (PubMed: 35122587) [IF=3.3]
4). Aspirin inhibits hypoxia-mediated lung cancer cell stemness and exosome function. Pathology - Research and Practice (PubMed: 30878308) [IF=2.8]

Application: WB    Species: human    Sample: A549 cells

Fig. 2.| Aspirin inhibits the hypoxia-enhanced stemness of A549 cells. (A–C) mRNA levels of SOX-2, OCT4, and ALDH1 were determined by qRT–PCR using specific primers, with GAPDH serving as the internal control. (D, E) mRNA levels of HIF-1α and COX-2 were determined by qRT–PCR using specific primers, with GAPDH serving as the internal control. (F) The concentration of PGE2 secreted by A549 cells was determined by ELISA. Each bar indicates the mean ± SD of n = 3 experiments. * indicates P < 0.05, ** indicates P < 0.01. (G) SOX-2, OCT4, ALDH1, HIF-1α, and COX-2 protein levels were determined by Western blot, with GAPDH serving as the loading control.

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