ZO 1 Antibody | Affinity Biosciences

Product:	ZO 1 Antibody
Catalog:	AF5145
Description:	Rabbit polyclonal antibody to ZO 1
Application:	WB IHC IF/ICC
Reactivity:	Human, Mouse, Rat, Pig, Monkey
Prediction:	Horse, Dog
Mol.Wt.:	195 kDa; 195kD(Calculated).
Uniprot:	Q07157
RRID:	AB_2837631

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

WB Handbook

IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat,Pig,Monkey

Prediction:

Horse(85%), Dog(92%)

Clonality:

Polyclonal

Specificity:

ZO 1 Antibody detects endogenous levels of total ZO 1.

RRID:

AB_2837631
Cite Format: Affinity Biosciences Cat# AF5145, RRID:AB_2837631.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Tight junction protein 1; Tight junction protein ZO-1; Tight junction protein ZO1; TJP1; zo-1; Zo1; ZO1_HUMAN; Zona occludens 1; Zona occludens 1 protein; Zona occludens protein 1; Zonula occludens 1 protein; Zonula occludens protein 1;

Immunogens

Immunogen:

Uniprot:

Q07157(ZO1_HUMAN) >>Visit HPA database.

Gene(ID):

TJP1(7082)

Expression:

Q07157 ZO1_HUMAN:

The alpha-containing isoform is found in most epithelial cell junctions. The short isoform is found both in endothelial cells and the highly specialized epithelial junctions of renal glomeruli and Sertoli cells of the seminiferous tubules.

Description:

The N-terminal may be involved in transducing a signal required for tight junction assembly, while the C-terminal may have specific properties of tight junctions. The alpha domain might be involved in stabilizing junctions.

Sequence:

Q07157 ZO1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MSARAAAAKSTAMEETAIWEQHTVTLHRAPGFGFGIAISGGRDNPHFQSGETSIVISDVLKGGPAEGQLQENDRVAMVNGVSMDNVEHAFAVQQLRKSGKNAKITIRRKKKVQIPVSRPDPEPVSDNEEDSYDEEIHDPRSGRSGVVNRRSEKIWPRDRSASRERSLSPRSDRRSVASSQPAKPTKVTLVKSRKNEEYGLRLASHIFVKEISQDSLAARDGNIQEGDVVLKINGTVTENMSLTDAKTLIERSKGKLKMVVQRDERATLLNVPDLSDSIHSANASERDDISEIQSLASDHSGRSHDRPPRRSRSRSPDQRSEPSDHSRHSPQQPSNGSLRSRDEERISKPGAVSTPVKHADDHTPKTVEEVTVERNEKQTPSLPEPKPVYAQVGQPDVDLPVSPSDGVLPNSTHEDGILRPSMKLVKFRKGDSVGLRLAGGNDVGIFVAGVLEDSPAAKEGLEEGDQILRVNNVDFTNIIREEAVLFLLDLPKGEEVTILAQKKKDVYRRIVESDVGDSFYIRTHFEYEKESPYGLSFNKGEVFRVVDTLYNGKLGSWLAIRIGKNHKEVERGIIPNKNRAEQLASVQYTLPKTAGGDRADFWRFRGLRSSKRNLRKSREDLSAQPVQTKFPAYERVVLREAGFLRPVTIFGPIADVAREKLAREEPDIYQIAKSEPRDAGTDQRSSGIIRLHTIKQIIDQDKHALLDVTPNAVDRLNYAQWYPIVVFLNPDSKQGVKTMRMRLCPESRKSARKLYERSHKLRKNNHHLFTTTINLNSMNDGWYGALKEAIQQQQNQLVWVSEGKADGATSDDLDLHDDRLSYLSAPGSEYSMYSTDSRHTSDYEDTDTEGGAYTDQELDETLNDEVGTPPESAITRSSEPVREDSSGMHHENQTYPPYSPQAQPQPIHRIDSPGFKPASQQKAEASSPVPYLSPETNPASSTSAVNHNVNLTNVRLEEPTPAPSTSYSPQADSLRTPSTEAAHIMLRDQEPSLSSHVDPTKVYRKDPYPEEMMRQNHVLKQPAVSHPGHRPDKEPNLTYEPQLPYVEKQASRDLEQPTYRYESSSYTDQFSRNYEHRLRYEDRVPMYEEQWSYYDDKQPYPSRPPFDNQHSQDLDSRQHPEESSERGYFPRFEEPAPLSYDSRPRYEQAPRASALRHEEQPAPGYDTHGRLRPEAQPHPSAGPKPAESKQYFEQYSRSYEQVPPQGFTSRAGHFEPLHGAAAVPPLIPSSQHKPEALPSNTKPLPPPPTQTEEEEDPAMKPQSVLTRVKMFENKRSASLETKKDVNDTGSFKPPEVASKPSGAPIIGPKPTSQNQFSEHDKTLYRIPEPQKPQLKPPEDIVRSNHYDPEEDEEYYRKQLSYFDRRSFENKPPAHIAASHLSEPAKPAHSQNQSNFSSYSSKGKPPEADGVDRSFGEKRYEPIQATPPPPPLPSQYAQPSQPVTSASLHIHSKGAHGEGNSVSLDFQNSLVSKPDPPPSQNKPATFRPPNREDTAQAAFYPQKSFPDKAPVNGTEQTQKTVTPAYNRFTPKPYTSSARPFERKFESPKFNHNLLPSETAHKPDLSSKTPTSPKTLVKSHSLAQPPEFDSGVETFSIHAEKPKYQINNISTVPKAIPVSPSAVEEDEDEDGHTVVATARGIFNSNGGVLSSIETGVSIIIPQGAIPEGVEQEIYFKVCRDNSILPPLDKEKGETLLSPLVMCGPHGLKFLKPVELRLPHCDPKTWQNKCLPGDPNYLVGANCVSVLIDHF

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Dog

Horse

Chicken

Rabbit

Pig

Bovine

Sheep

Xenopus

Zebrafish

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q07157 As Substrate

Q07157

Site	PTM Type	Source
T16	Phosphorylation	Uniprot
S53	Phosphorylation	Uniprot
S117	Phosphorylation	Uniprot
S125	Phosphorylation	Uniprot
S131	Phosphorylation	Uniprot
Y132	Phosphorylation	Uniprot
S141	Phosphorylation	Uniprot
S144	Phosphorylation	Uniprot
S151	Phosphorylation	Uniprot
K153	Ubiquitination	Uniprot
S160	Phosphorylation	Uniprot
S162	Phosphorylation	Uniprot
S166	Phosphorylation	Uniprot
S168	Phosphorylation	Uniprot
S175	Phosphorylation	Uniprot
S178	Phosphorylation	Uniprot
S179	Phosphorylation	Uniprot
K183	Ubiquitination	Uniprot
T185	Phosphorylation	Uniprot
K194	Ubiquitination	Uniprot
S212	Phosphorylation	Uniprot
S215	Phosphorylation	Uniprot
S241	Phosphorylation	Uniprot
T267	Phosphorylation	Uniprot
S275	Phosphorylation	Uniprot
S277	Phosphorylation	Uniprot
S280	Phosphorylation	Uniprot
S284	Phosphorylation	Uniprot
S290	Phosphorylation	Uniprot
S294	Phosphorylation	Uniprot
S297	Phosphorylation	Uniprot
S300	Phosphorylation	Uniprot
S313	Phosphorylation	Uniprot
S315	Phosphorylation	Uniprot
S320	Phosphorylation	Uniprot
S323	Phosphorylation	Uniprot
S329	Phosphorylation	Uniprot
S334	Phosphorylation	Uniprot
S337	Phosphorylation	Uniprot
S340	Phosphorylation	Uniprot
S347	Phosphorylation	Uniprot
K348	Ubiquitination	Uniprot
S353	Phosphorylation	Uniprot
T354	Phosphorylation	Uniprot
T363	Phosphorylation	Uniprot
T379	Phosphorylation	Uniprot
S381	Phosphorylation	Uniprot
Y389	Phosphorylation	Uniprot
S402	Phosphorylation	Uniprot
S421	Phosphorylation	Uniprot
S432	Phosphorylation	Uniprot
T476	Phosphorylation	Uniprot
S513	Phosphorylation	Uniprot
Y520	Phosphorylation	Uniprot
T548	Phosphorylation	Uniprot
Y550	Phosphorylation	Uniprot
K553	Ubiquitination	Uniprot
S585	Phosphorylation	Uniprot
Y588	Phosphorylation	Uniprot
T589	Phosphorylation	Uniprot
K592	Ubiquitination	Uniprot
S617	Phosphorylation	Uniprot
S622	Phosphorylation	Uniprot
T628	Phosphorylation	Uniprot
Y633	Phosphorylation	Uniprot
T648	Phosphorylation	Uniprot
Y669	Phosphorylation	Uniprot
K673	Ubiquitination	Uniprot
S686	Phosphorylation	Uniprot
K695	Ubiquitination	Uniprot
K702	Ubiquitination	Uniprot
T709	Phosphorylation	Uniprot
Y718	Phosphorylation	Uniprot
K733	Acetylation	Uniprot
K737	Acetylation	Uniprot
T738	Phosphorylation	Uniprot
T770	Phosphorylation	Uniprot
T772	Phosphorylation	Uniprot
T809	Phosphorylation	Uniprot
S810	Phosphorylation	Uniprot
S821	Phosphorylation	Uniprot
Y822	Phosphorylation	Uniprot
S824	Phosphorylation	Uniprot
S828	Phosphorylation	Uniprot
Y830	Phosphorylation	Uniprot
S831	Phosphorylation	Uniprot
Y833	Phosphorylation	Uniprot
S834	Phosphorylation	Uniprot
S837	Phosphorylation	Uniprot
Y843	Phosphorylation	Uniprot
T854	Phosphorylation	Uniprot
T861	Phosphorylation	Uniprot
T868	Phosphorylation	Uniprot
S877	Phosphorylation	Uniprot
S886	Phosphorylation	Uniprot
T894	Phosphorylation	Uniprot
Y895	Phosphorylation	Uniprot
Y898	Phosphorylation	Uniprot
S899	Phosphorylation	Uniprot
S912	Phosphorylation	Uniprot
S919	Phosphorylation	Uniprot
S926	Phosphorylation	Uniprot
S927	Phosphorylation	Uniprot
Y931	Phosphorylation	Uniprot
S933	Phosphorylation	Uniprot
T936	Phosphorylation	Uniprot
S964	Phosphorylation	Uniprot
T965	Phosphorylation	Uniprot
S966	Phosphorylation	Uniprot
Y967	Phosphorylation	Uniprot
S968	Phosphorylation	Uniprot
S973	Phosphorylation	Uniprot
T976	Phosphorylation	Uniprot
S978	Phosphorylation	Uniprot
T979	Phosphorylation	Uniprot
S992	Phosphorylation	Uniprot
S994	Phosphorylation	Uniprot
S1025	Phosphorylation	Uniprot
Y1039	Phosphorylation	Uniprot
S1051	Phosphorylation	Uniprot
T1058	Phosphorylation	Uniprot
Y1059	Phosphorylation	Uniprot
Y1061	Phosphorylation	Uniprot
S1064	Phosphorylation	Uniprot
S1065	Phosphorylation	Uniprot
Y1066	Phosphorylation	Uniprot
T1067	Phosphorylation	Uniprot
S1071	Phosphorylation	Uniprot
Y1074	Phosphorylation	Uniprot
Y1087	Phosphorylation	Uniprot
S1092	Phosphorylation	Uniprot
S1102	Phosphorylation	Uniprot
S1111	Phosphorylation	Uniprot
Y1128	Phosphorylation	Uniprot
S1139	Phosphorylation	Uniprot
Y1140	Phosphorylation	Uniprot
S1142	Phosphorylation	Uniprot
Y1146	Phosphorylation	Uniprot
S1153	Phosphorylation	Uniprot
Y1165	Phosphorylation	Uniprot
T1167	Phosphorylation	Uniprot
S1180	Phosphorylation	Uniprot
K1189	Ubiquitination	Uniprot
Y1191	Phosphorylation	Uniprot
Y1195	Phosphorylation	Uniprot
S1196	Phosphorylation	Uniprot
S1198	Phosphorylation	Uniprot
Y1199	Phosphorylation	Uniprot
K1269	Ubiquitination	Uniprot
S1278	Phosphorylation	Uniprot
K1321	Ubiquitination	Uniprot
T1322	Phosphorylation	Uniprot
Y1324	Phosphorylation	Uniprot
K1331	Ubiquitination	Uniprot
Y1346	Phosphorylation	Uniprot
Y1354	Phosphorylation	Uniprot
Y1355	Phosphorylation	Uniprot
K1357	Ubiquitination	Uniprot
Y1361	Phosphorylation	Uniprot
S1366	Phosphorylation	Uniprot
S1397	Phosphorylation	Uniprot
Y1398	Phosphorylation	Uniprot
S1399	Phosphorylation	Uniprot
S1413	Phosphorylation	Uniprot
K1417	Acetylation	Uniprot
Y1419	Phosphorylation	Uniprot
T1425	Phosphorylation	Uniprot
S1433	Phosphorylation	Uniprot
Y1435	Phosphorylation	Uniprot
S1439	Phosphorylation	Uniprot
T1443	Phosphorylation	Uniprot
S1444	Phosphorylation	Uniprot
S1446	Phosphorylation	Uniprot
S1451	Phosphorylation	Uniprot
S1503	Phosphorylation	Uniprot
T1521	Phosphorylation	Uniprot
Y1524	Phosphorylation	Uniprot
T1528	Phosphorylation	Uniprot
K1530	Methylation	Uniprot
Y1532	Phosphorylation	Uniprot
S1534	Phosphorylation	Uniprot
S1545	Phosphorylation	Uniprot
T1567	Phosphorylation	Uniprot
T1569	Phosphorylation	Uniprot
S1570	Phosphorylation	Uniprot
T1573	Phosphorylation	Uniprot
S1577	Phosphorylation	Uniprot
S1579	Phosphorylation	Uniprot
S1588	Phosphorylation	Uniprot
S1594	Phosphorylation	Uniprot
K1601	Ubiquitination	Uniprot
Y1602	Phosphorylation	Uniprot
S1617	Phosphorylation	Uniprot
S1619	Phosphorylation	Uniprot
S1655	Phosphorylation	Uniprot
K1687	Ubiquitination	Uniprot
K1709	Ubiquitination	Uniprot

Research Backgrounds

Q07157_ZO1_HUMAN

Function:

TJP1, TJP2, and TJP3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton. The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity). Plays a role in the regulation of cell migration by targeting CDC42BPB to the leading edge of migrating cells. Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling. With TJP2 and TJP3, participates to the junctional retention and stability of the transcription factor DBPA, but is not involved in its shuttling to the nucleus (By similarity).

PTMs:

Phosphorylated at tyrosine redidues in response to epidermal growth factor (EGF). This response is dependent on an intact actin microfilament system. Dephosphorylated by PTPRJ.

Subcellular Location:

Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cell junction>Tight junction. Cell junction. Cell junction>Gap junction. Cell projection>Podosome.
Note: Moves from the cytoplasm to the cell membrane concurrently with cell-cell contact (PubMed:7798316). At podosomal sites, is predominantly localized in the ring structure surrounding the actin core (PubMed:20930113).

Tissue Specificity:

Subunit Structure:

Homodimer. Forms heterodimers (via the PDZ2 domain) with TJP2 and TJP3. Interacts with OCLN, CALM, claudins, CGN/cingulin, CXADR, GJA12, GJD3 and UBN1. Interacts (via ZU5 domain) with CDC42BPB and MYZAP. Interacts (via PDZ domain) with GJA1. Interacts (via PDZ domains) with ANKRD2. Interacts with BVES (via the C-terminus cytoplasmic tail) (By similarity). Interacts with HSPA4 and KIRREL1 (By similarity). Interacts with DLL1 (By similarity). Interacts with USP53 (via the C-terminal region) (By similarity). Interacts (via ABR region) with F-actin. Interacts with DNMBP (via C-terminal domain); required for the apical cell-cell junction localization of DNMBP.

Family&Domains:

The 244-aa domain between residues 633 and 876 is the primary occludin (OCLN)-binding site and is required for stable association with the tight junction (PubMed:9792688).

The C-terminal region (residues 1151-1372) is an actin-binding region (ABR) that interacts directly with F-actin and plays an important role in the localization of TJP1 at junctions (PubMed:9792688, PubMed:12354695, PubMed:20930113). The ABR is also required for the localization to puncta at the free edge of cells before initiation of cell-cell contact (PubMed:12354695). The ABR is also necessary for TJP1 recruitment to podosomes (PubMed:20930113).

The second PDZ domain (PDZ2) mediates homodimerization and heterodimerization with TJP2 and TJP3 (PubMed:9792688, PubMed:17928286).

Belongs to the MAGUK family.

Research Fields

· Cellular Processes > Cellular community - eukaryotes > Adherens junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Tight junction. (View pathway)

· Cellular Processes > Cellular community - eukaryotes > Gap junction. (View pathway)

· Human Diseases > Infectious diseases: Bacterial > Vibrio cholerae infection.

· Human Diseases > Infectious diseases: Bacterial > Epithelial cell signaling in Helicobacter pylori infection.

· Human Diseases > Infectious diseases: Bacterial > Salmonella infection.

References

1). A Nucleophilicity-Engineered DNA Ligation Blockade Nanoradiosensitizer Induces Irreversible DNA Damage to Overcome Cancer Radioresistance. Advanced materials (Deerfield Beach, Fla.), 2024 (PubMed: 39246208) [IF=27.4]

2). Reinforcement of the intestinal mucosal barrier via mucus-penetrating PEGylated bacteria. Nature biomedical engineering, 2024 (PubMed: 38839928) [IF=26.8]

3). Chemical reaction-mediated covalent localization of bacteria. Nature Communications, 2022 (PubMed: 36528693) [IF=16.6]

4). The role of the microbiota–gut–brain axis in methamphetamine-induced neurotoxicity: Disruption of microbial composition and short-chain fatty acid metabolism. Acta Pharmaceutica Sinica B, 2024 [IF=14.7]

5). Extracellular vesicles of Fusobacterium nucleatum compromise intestinal barrier through targeting RIPK1-mediated cell death pathway. Gut Microbes, 2021 (PubMed: 33769187) [IF=12.2]

Application: WB Species: human Sample: Caco2 cells

Figure 2. |FnEVs promote TNF-α induced Caco2 cells death and barrier loss.(i) Immunofluorescence results of immunofluorescence localization of the TJ protein ZO-1(red, ZO-1 positive; blue, DAPI) and immunoblot analysis of ZO-1 protein. Scale bar = 50 um. *p < .05, **p < .01, ***p < .001. All the results were repeated three times. All data were presented as means ± SD (n = 3). TEER, transepithelial electrical resistance measurements.

Application: IF/ICC Species: human Sample: Caco2 cells

Application: IHC Species: mouse Sample: colon

Figure 5. |FnEVs increase gut barrier leakage in experimental colitis models. (a) Representative images and ex vivo imaging with the intestine, liver, heart, spleen and kidney of mice. (b) Relative fluorescence intensity of translocated EGFP-labeled E.coli in every tissues. (c) Representative images of immunohistochemical stainings of ZO-1, claudin-1 and occludin in the colon on day 3 after colitis induction. Scale bar = 50 um.

6). Versatility of bacterial outer membrane vesicles in regulating intestinal homeostasis. Science Advances, 2023 (PubMed: 36921043) [IF=11.7]

7). Targeted delivery of Chinese herb pair-based berberine/tannin acid self-assemblies for the treatment of ulcerative colitis. Journal of Advanced Research, 2022 (PubMed: 36100331) [IF=11.4]

8). Water-extracted Lonicera japonica polysaccharide attenuates allergic rhinitis by regulating NLRP3-IL-17 signaling axis. Carbohydrate Polymers, 2022 (PubMed: 36184153) [IF=10.7]

9). Succinate exacerbates mastitis in mice via extracellular vesicles derived from the gut microbiota: a potential new mechanism for mastitis.. Journal of nanobiotechnology, 2024 (PubMed: 39543623) [IF=10.2]

Application: WB Species: Mouse Sample:

Fig. 2 Succinate facilitates endotoxemia-induced systemic immune imbalance and intestinal barrier disruption in mice. (A) Representative H&E-stained liver sections from the indicated mice (scale bar, 50 μm). (B) Representative H&E-stained colons sections from different groups (scale bar, 50 μm). (C) Representative AB-stained colons sections (scale bar, 50 μm). (D) Histological score of the liver based on H&E staining. (E) Histological scores of the colons based on H&E staining. (F and G) Levels of serum IL-1β and TNF-α. (H-K) Protein levels of colon Claudin-3, Occludin, and ZO-1 were measured by western blotting and intensity analysis. Data are expressed as the mean ± SD and one-way ANOVA was performed, followed by Tukey’s test (n = 3–6)

10). Stem cell membrane-coated isotretinoin for acne treatment. JOURNAL OF NANOBIOTECHNOLOGY, 2020 (PubMed: 32723398) [IF=10.2]

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ZO 1 Antibody - #AF5145