Product: Lamin B1 Antibody
Catalog: AF5161
Description: Rabbit polyclonal antibody to Lamin B1
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat, Monkey, Fish
Prediction: Pig, Bovine, Sheep, Rabbit, Xenopus
Mol.Wt.: 66 kDa; 66kD(Calculated).
Uniprot: P20700
RRID: AB_2837647

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 50ul $150 In stock
 100ul $250 In stock
 200ul $350 In stock
 1ml $1200 In stock

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey,Fish
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(100%), Xenopus(83%)
Clonality:
Polyclonal
Specificity:
Lamin B1 Antibody detects endogenous levels of total Lamin B.
RRID:
AB_2837647
Cite Format: Affinity Biosciences Cat# AF5161, RRID:AB_2837647.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ADLD; lamin B1; Lamin-B1; LMN; LMN2; LMNB; Lmnb1; LMNB1_HUMAN; MGC111419; OTTHUMP00000159218;

Immunogens

Immunogen:

A synthesized peptide derived from human Lamin B1.

Uniprot:
Gene(ID):
Description:
Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.
Sequence:
MATATPVPPRMGSRAGGPTTPLSPTRLSRLQEKEELRELNDRLAVYIDKVRSLETENSALQLQVTEREEVRGRELTGLKALYETELADARRALDDTARERAKLQIELGKCKAEHDQLLLNYAKKESDLNGAQIKLREYEAALNSKDAALATALGDKKSLEGDLEDLKDQIAQLEASLAAAKKQLADETLLKVDLENRCQSLTEDLEFRKSMYEEEINETRRKHETRLVEVDSGRQIEYEYKLAQALHEMREQHDAQVRLYKEELEQTYHAKLENARLSSEMNTSTVNSAREELMESRMRIESLSSQLSNLQKESRACLERIQELEDLLAKEKDNSRRMLTDKEREMAEIRDQMQQQLNDYEQLLDVKLALDMEISAYRKLLEGEEERLKLSPSPSSRVTVSRASSSRSVRTTRGKRKRVDVEESEASSSVSISHSASATGNVCIEEIDVDGKFIRLKNTSEQDQPMGGWEMIRKIGDTSVSYKYTSRYVLKAGQTVTIWAANAGVTASPPTDLIWKNQNSWGTGEDVKVILKNSQGEEVAQRSTVFKTTIPEEEEEEEEAAGVVVEEELFHQQGTPRASNRSCAIM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Rabbit
100
Xenopus
83
Chicken
70
Zebrafish
58
Horse
0
Dog
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Lamins are components of the nuclear lamina, a fibrous layer on the nucleoplasmic side of the inner nuclear membrane, which is thought to provide a framework for the nuclear envelope and may also interact with chromatin.

PTMs:

B-type lamins undergo a series of modifications, such as farnesylation and phosphorylation. Increased phosphorylation of the lamins occurs before envelope disintegration and probably plays a role in regulating lamin associations.

Subcellular Location:

Nucleus inner membrane>Lipid-anchor>Nucleoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Family&Domains:

Belongs to the intermediate filament family.

Research Fields

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

References

1). Enzymatically extracted ulvans restrict viruses via STING signaling and type I interferon after cellular entry. Carbohydrate polymers, 2025 (PubMed: 39562059) [IF=10.7]

2). Micheliolide Ameliorates Diabetic Kidney Disease by Inhibiting Mtdh-mediated Renal Inflammation in Type 2 Diabetic db/db Mice. PHARMACOLOGICAL RESEARCH, 2019 (PubMed: 31669149) [IF=9.1]

3). Inhibition of fatty acid catabolism augments the efficacy of oxaliplatin-based chemotherapy in gastrointestinal cancers. CANCER LETTERS, 2020 (PubMed: 31904482) [IF=9.1]

Application: WB    Species: Human    Sample: HGC27 cells

Fig. 7. NFATc3 promoted CPT2 transcription in gastrointestinal cancers. (A) The correlation between CPT2 and NFATc3 in GC and colon adenocarcinoma (COAD) from the GEPIA database. The r values and P values are from Pearson's correlation analysis. (B) The correlation between CPT2 and NFATc3 in GC (n = 36) and CRC (n = 55) samples. The r values and P values are from Pearson's correlation analysis. (C) CPT2 levels were downregulated when NFATc3 was knocked down in the indicated cells. Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01 versus the control. (D) Western blot analysis shows the NFATc3 content in the cytoplasm and nucleus of HGC27 cells treated with or without oxaliplatin (Oxa, 40 μM) for 24 h. Lamin B was used as the nuclear loading control with α- Tubulin as the cytosolic loading control. (E) NFATc3 DNA-binding sites are present in the human CPT2 promoter region. (F) ChIP-PCR in HGC27 and HCT116 cells. The data are representative of two independent experiments. *P < 0.05 or **P < 0.01. (G) Relative CPT2 luciferase promoter activity in the indicated cells with NFATc3 depletion or overexpression. Error bars, SD of three independent experiments. *P < 0.05 or **P < 0.01 versus the control. (H) Proposed working model from this study. Oxaliplatin-induced ROS generation causes the accumulation of NFATc3 in the nucleus, promoting the CPT2 transcription. Perhexiline, as a CPT inhibitor, blocks FA catabolism in mitochondria, resulting in reduced NADPH levels. Therefore, increased ROS caused the apoptosis in gastrointestinal cancers.

4). Preparation of Phillygenin-Hyaluronic acid composite milk-derived exosomes and its anti-hepatic fibrosis effect. Materials today. Bio, 2023 (PubMed: 37753374) [IF=8.7]

5). Phillygenin inhibited M1 macrophage polarization and reduced hepatic stellate cell activation by inhibiting macrophage exosomal miR-125b-5p. BIOMEDICINE & PHARMACOTHERAPY, 2023 (PubMed: 36652738) [IF=6.9]

Application: WB    Species: Mouse    Sample: mHSCs

Fig. 3. PHI treatment reduced M1 macrophage-induced HSC activation. (a) mHSCs were co-cultured with the CMs from RAW264.7 cells with LPS/IFNγ and PHI treatment for 12 h. (b-g) The expression of MMP2, TIMP1, TGF-β, α-SMA, COL1 and NF-κB mRNA in mHSCs after co-culture with macrophage-derived CMs for 24 h was detected by RT-qPCR (n = 3). (h) The expression of TGF-β, α-SMA, COL1, P65 and P-P65 proteins in mHSCs after co-culture with macrophage-derived CMs for 24 h was detected by western blotting. (i) The relative quantification of TGF-β, α-SMA, COL1 and P-P65/P65 protein expression in western blotting results was analyzed by ImageJ software (n = 3). (j) The expression of cytoplasm and nucleus NF-κB P65 proteins in mHSCs after co-culture with macrophage-derived CMs for 24 h was detected by western blotting. (k, l) The relative quantification of cytoplasm and nucleus NF-κB P65 protein expression in western blotting results was analyzed by ImageJ software (n = 3). (m) Immunofluorescence staining of α-SMA in mHSCs after co-culture with CMs from RAW264.7 cells with different treatments for 24 h (Scale bar=100 µm). Results are presented as mean ± SD. ###P 

6). Ellagic acid ameliorates arsenic-induced neuronal ferroptosis and cognitive impairment via Nrf2/GPX4 signaling pathway. Ecotoxicology and environmental safety, 2024 (PubMed: 39128446) [IF=6.2]

Application: WB    Species: Rat    Sample: hippocampus and HT22 cells

Fig. 6. The impact of ellagic acid treatment on arsenic-induced Nrf2/Keap1 signaling pathway in the hippocampus and HT22 cells. (A) Western blot for p-Nrf2, Nrf2 and Keap1 in the hippocampus. (B-D) Relative density analysis of the p-Nrf2, Nrf2 and Keap1 protein bands in the hippocampus. (E) Quantification of Keap1 mRNA levels in the hippocampus. (F) Western blot for Nucleus Nrf2, Nrf2 and Keap1 in HT22 cells. (G-I) Relative density analysis of the Nucleus Nrf2, Nrf2 and Keap1 protein bands in HT22 cells. (J) Quantification of Keap1 mRNA levels in HT22 cells. Data are presented as mean ± SD (n=3). * significant difference compared to the control group (*P

7). MCP-enhanced SOD3 activity inhibits gastric cancer and potentiate chemotherapy via modulating EGFR signaling. Life sciences, 2024 (PubMed: 39746602) [IF=6.1]

8). Coriolus versicolor alleviates diabetic cardiomyopathy by inhibiting cardiac fibrosis and NLRP3 inflammasome activation. PHYTOTHERAPY RESEARCH, 2019 (PubMed: 31338905) [IF=6.1]

9). Chang qing formula ameliorates colitis-associated colorectal cancer via suppressing IL-17/NF-κB/STAT3 pathway in mice as revealed by network pharmacology study. Frontiers in Pharmacology, 2022 (PubMed: 35991881) [IF=5.6]

Application: WB    Species: Mice    Sample: colon tissue

FIGURE 6 CQF decreased the level of IL-17A and impeded the activation of NF-kB/IL-6/STAT3 signaling cascade. (A) IL-17A concentrations in serum samples. (B–C) Expression of IL-17RA protein in colon tissue samples (n = 4 per group). (D) IL-6 concentrations in serum samples (n = 6 per group). (E,F) Nuclear expression of NF-κB-p65 and p-STAT3 proteins in colon tissue samples (n = 4 per group). Results are expressed as mean ± SEM. # p < 0.05, ## p < 0.01, compared with normal mice; * p < 0.05, ** p < 0.01, compared with AOM/DSS-treated mice.

10). Ellagic Acid Attenuates BLM-Induced Pulmonary Fibrosis via Inhibiting Wnt Signaling Pathway. Frontiers in Pharmacology, 2021 (PubMed: 33912053) [IF=5.6]

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