S100A10 Antibody - #AF5180

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Product: S100A10 Antibody
Catalog: AF5180
Description: Rabbit polyclonal antibody to S100A10
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 11 kDa; 11kD(Calculated).
Uniprot: P60903
RRID: AB_2837666

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
S100A10 Antibody detects endogenous levels of total S100A10.
RRID:
AB_2837666
Cite Format: Affinity Biosciences Cat# AF5180, RRID:AB_2837666.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

42C; AA409961; AL024248; Annexin II ligand; Annexin II ligand, calpactin I, light polypeptide; Annexin II tetramer (AIIt) p11 subunit; Annexin II, light chain; ANX2L; ANX2LG; Ca[1]; CAL12; CAL1L; Calpactin I light chain; Calpactin I, p11 subunit; Calpactin-1 light chain; Cellular ligand of annexin II; CLP11; GP11; MGC111133; Nerve growth factor-induced protein 42C; OTTHUMP00000015269; OTTHUMP00000015270; p10; p10 protein; p11; Protein S100 A10; Protein S100-A10; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10 (calpactin); S100 calcium binding protein A10; S100 calcium-binding protein A10; S100a10; S10AA_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human S100A10, corresponding to a region within the internal amino acids.

Uniprot:

P60903(S10AA_HUMAN) >>Visit HPA database.

Gene(ID):
S100A10(6281)
Description:
Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.
Sequence:
MPSQMEHAMETMMFTFHKFAGDKGYLTKEDLRVLMEKEFPGFLENQKDPLAVDKIMKDLDQCRDGKVGFQSFFSLIAGLTIACNDYFVVHMKQKGKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
77
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine-specific kinase.

Family&Domains:

Belongs to the S-100 family.

References

1). Notch signaling activation contributes to paclitaxel-induced neuropathic pain via activation of A1 astrocytes. European journal of pharmacology, 2022 (PubMed: 35777441) [IF=4.2]

Application: WB    Species: Rat    Sample: spinal cord

Fig. 2. Astrocytes were activated as the A1 phenotype in spinal cord. (A–E) The spinal expression of GFAP, C3 and Serping1 were significantly increased in spinal cord at 14 d and 21 d after intraperitoneal injection of paclitaxel, while the spinal S100A10 and PTX3 were decreased at 14 d and 21 d (*p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group, n=6 in each group). (F–G) Dual-label immunofluorescence showed that A1 astrocytes markers (C3, Serping1) and A2 astrocytes markers (S100A10, PTX3) were mostly colocalized with GFAP in the dorsal horn of spinal cord. Scale Bar: 50 μm and 100 μm, respectively (n=4). The white arrow indicated typical co-staining cells.

Application: IF/ICC    Species: Rat    Sample: spinal cord

Fig. 2. Astrocytes were activated as the A1 phenotype in spinal cord. (A–E) The spinal expression of GFAP, C3 and Serping1 were significantly increased in spinal cord at 14 d and 21 d after intraperitoneal injection of paclitaxel, while the spinal S100A10 and PTX3 were decreased at 14 d and 21 d (*p < 0.05, **p < 0.01, ***p < 0.001 compared with the vehicle group, n=6 in each group). (F–G) Dual-label immunofluorescence showed that A1 astrocytes markers (C3, Serping1) and A2 astrocytes markers (S100A10, PTX3) were mostly colocalized with GFAP in the dorsal horn of spinal cord. Scale Bar: 50 μm and 100 μm, respectively (n=4). The white arrow indicated typical co-staining cells.

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