Cathepsin B Antibody - #AF5189

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Product: Cathepsin B Antibody
Catalog: AF5189
Description: Rabbit polyclonal antibody to Cathepsin B
Application: WB IHC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 37 kDa; 38kD(Calculated).
Uniprot: P07858
RRID: AB_2837675

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
Cathepsin B Antibody detects endogenous levels of total Cathepsin B.
RRID:
AB_2837675
Cite Format: Affinity Biosciences Cat# AF5189, RRID:AB_2837675.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Amyloid precursor protein secretase; APP secretase; APPS; CATB_HUMAN; Cathepsin B heavy chain; Cathepsin B1; CathepsinB; CPSB; CTSB; cysteine protease; OTTHUMP00000116009; OTTHUMP00000229510; OTTHUMP00000229511; OTTHUMP00000229512; OTTHUMP00000229514; OTTHUMP00000229515; OTTHUMP00000229516; Preprocathepsin B;

Immunogens

Immunogen:

A synthesized peptide derived from human Cathepsin B, corresponding to a region within the internal amino acids.

Uniprot:

P07858(CATB_HUMAN) >>Visit HPA database.

Gene(ID):
CTSB(1508)
Expression:
P07858 CATB_HUMAN:

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

Description:
Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis.
Sequence:
MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI

Research Backgrounds

Function:

Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen (By similarity). Has also been implicated in tumor invasion and metastasis.

Subcellular Location:

Lysosome. Melanosome. Secreted>Extracellular space. Apical cell membrane>Peripheral membrane protein>Extracellular side.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:17081065). Localizes to the lumen of thyroid follicles and to the apical membrane of thyroid epithelial cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the stratum spinosum of the epidermis. Weak expression is detected in the stratum granulosum.

Family&Domains:

Belongs to the peptidase C1 family.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

· Cellular Processes > Transport and catabolism > Lysosome.   (View pathway)

· Cellular Processes > Cell growth and death > Apoptosis.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

· Organismal Systems > Immune system > NOD-like receptor signaling pathway.   (View pathway)

· Organismal Systems > Endocrine system > Renin secretion.

References

1). Resistant Starch Nanoparticles Induce Colitis through Lysosomal Exocytosis in Mice. ACS nano, 2024 (PubMed: 39442088) [IF=15.8]
2). Physical Exercise Decreases Complement-Mediated Synaptic Loss and Protects Against Cognitive Impairment by Inhibiting Microglial Tmem9-ATP6V0D1 in Alzheimer's Disease. Aging cell, 2025 (PubMed: 39871402) [IF=8.0]
3). Strontium Regulates the Proliferation and Differentiation of Isolated Primary Bovine Chondrocytes via the TGFβ/SMAD Pathway. Frontiers in pharmacology, 2022 (PubMed: 35712700) [IF=5.6]

Application: WB    Species: Bovine    Sample:

FIGURE 2. Effects of Sr and SB-505124 on the transforming growth factor β (TGFβ)/SMAD family member (SMAD) signaling pathway and downstream gene transcription in bovine chondrocytes. Chondrocytes were treated with different doses of Sr (0, 0.1, 1, 10 μg/ml) with or without activin receptor-like kinase 5 (ALK5) kinase inhibitor (10 μM SB-505124). (A) Western blot analysis and relative protein expression levels of SMAD3, pSMAD3, SMAD1/5/9, pSMAD1/5/9, TGFβ, bone morphogenetic protein 2 (BMP2), and runt-related transcription factor 2 (RUNX2). (B) mRNA expression levels of TGFβ1, serpin family E member 1 (SERPINE1), ALK5, ALK1, and inhibitor of DNA binding 1 (ID1). All experiments were repeated at least thrice. Data are means ± SEM (n = 3 in each group) *p < 0.05; **p < 0.01.
4). Euphejolkinolide A, a new ent-abietane lactone from Euphorbia peplus L. with promising biological activity in activating the autophagy-lysosomal pathway. Heliyon, 2023 (PubMed: 36852065) [IF=4.0]

Application: WB    Species: human    Sample: U251 cells

Fig. 4 Compound 1 activates autophagy A. Western blot result shows the protein levels of CTSB (pro CTSB and mature CTSB), LC3 and SQSTM1 in the U251 cells treated with compound 1 (1, 1 μM, 10 μM and 40 μM) or rapamycin (Rapa, 2 μM and 5 μM). B. Quantifications of the protein levels of CTSB, SQSTM1 and LC3-II in the U251 cells in A based on 3 independent experiments. C. Western blot result shows the protein levels of CTSB, LC3 and SQSTM1 in the HM cells treated with 1 (1 μM, 10 μM and 40 μM) or Rapa (2 μM and 5 μM). D. Quantification of the protein levels of CTSB, SQSTM1 and LC3-II in the HM cells in C based on 3 independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001; ns, not significant; one-way ANOVA with the Dunnett's post hoc test. Bars represent mean ± SD.
5). Pelargonic acid vanillylamide alleviates hepatic autophagy and ER stress in hepatic steatosis model. Food and Chemical Toxicology, 2023 (PubMed: 37611858) [IF=3.9]
6). Knockdown of regulator of Calcineurin 2 promotes transcription factor EB-mediated lipophagy to prevent non-alcoholic fatty liver disease. Toxicology and applied pharmacology, 2025 (PubMed: 39710154) [IF=3.3]
7). Munronin V with 7/7/6 tricarbocyclic framework from Munronia henryi harms inhibits tau pathology by activating autophagy. Organic & biomolecular chemistry, 2023 (PubMed: 36594355) [IF=2.9]
8). TFE3 regulates the function of the autophagy-lysosome pathway to drive the invasion and metastasis of papillary thyroid carcinoma. Analytical Cellular Pathology, 2021 (PubMed: 34660181) [IF=2.6]

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