n-Myc Antibody - #AF5204

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Product: n-Myc Antibody
Catalog: AF5204
Description: Rabbit polyclonal antibody to n-Myc
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Chicken
Mol.Wt.: 50~65 kDa; 50kD(Calculated).
Uniprot: P04198
RRID: AB_2837690

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Sheep(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
n-Myc Antibody detects endogenous levels of total n-Myc.
RRID:
AB_2837690
Cite Format: Affinity Biosciences Cat# AF5204, RRID:AB_2837690.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

bHLHe37; Class E basic helix-loop-helix protein 37; MODED; mycn; MYCN_HUMAN; N myc; N myc proto oncogene protein; N-myc proto-oncogene protein; Neuroblastoma derived v myc avian myelocytomatosis viral related oncogene; Neuroblastoma MYC oncogene; NMYC; NMYC proto oncogene protein; ODED; Oncogene NMYC; pp65/67; V myc avian myelocytomatosis viral oncogene neuroblastoma derived homolog; v myc avian myelocytomatosis viral related oncogene neuroblastoma derived; v myc myelocytomatosis viral related oncogene neuroblastoma derived;

Immunogens

Immunogen:

A synthesized peptide derived from human n-Myc, corresponding to a region within the internal amino acids.

Uniprot:

P04198(MYCN_HUMAN) >>Visit HPA database.

Gene(ID):
MYCN(4613)
Expression:
P04198 MYCN_HUMAN:

Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).

Description:
Note=Amplification of the N-MYC gene is associated with a variety of human tumors, most frequently neuroblastoma, where the level of amplification appears to increase as the tumor progresses.
Sequence:
MPSCSTSTMPGMICKNPDLEFDSLQPCFYPDEDDFYFGGPDSTPPGEDIWKKFELLPTPPLSPSRGFAEHSSEPPSWVTEMLLENELWGSPAEEDAFGLGGLGGLTPNPVILQDCMWSGFSAREKLERAVSEKLQHGRGPPTAGSTAQSPGAGAASPAGRGHGGAAGAGRAGAALPAELAHPAAECVDPAVVFPFPVNKREPAPVPAAPASAPAAGPAVASGAGIAAPAGAPGVAPPRPGGRQTSGGDHKALSTSGEDTLSDSDDEDDEEEDEEEEIDVVTVEKRRSSSNTKAVTTFTITVRPKNAALGPGRAQSSELILKRCLPIHQQHNYAAPSPYVESEDAPPQKKIKSEASPRPLKSVIPPKAKSLSPRNSDSEDSERRRNHNILERQRRNDLRSSFLTLRDHVPELVKNEKAAKVVILKKATEYVHSLQAEEHQLLLEKEKLQARQQQLLKKIEHARTC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Chicken
83
Horse
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Positively regulates the transcription of MYCNOS in neuroblastoma cells.

PTMs:

Phosphorylated by GSK3-beta which may promote its degradation. Phosphorylated by AURKA.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in the neuronal cells of the cerebrum, neuroblastomas and thyroid tumors (at protein level).

Research Fields

· Human Diseases > Cancers: Overview > Transcriptional misregulation in cancer.

References

1). Demethylzeylasteral alleviates inflammation and colitis via dual suppression of NF-κB and STAT3/5 by targeting IKKα/β and JAK2. International immunopharmacology, 2024 (PubMed: 39340986) [IF=4.8]
2). Laminaria japonica fucoidan ameliorates cyclophosphamide‐induced liver and kidney injury possibly by regulating Nrf2/HO‐1 and TLR4/NF‐κB signaling pathways. Journal of the Science of Food and Agriculture, 2022 (PubMed: 34689333) [IF=3.3]

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