Product: HOXB5 Antibody
Catalog: AF5215
Description: Rabbit polyclonal antibody to HOXB5
Application: WB
Reactivity: Human, Mouse
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 29 kDa; 29kD(Calculated).
Uniprot: P09067
RRID: AB_2837701

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%), Xenopus(85%)
Clonality:
Polyclonal
Specificity:
HOXB5 Antibody detects endogenous levels of total HOXB5.
RRID:
AB_2837701
Cite Format: Affinity Biosciences Cat# AF5215, RRID:AB_2837701.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

HGNC:5116; HHO.C10; Homeo box 2A; Homeo box B5; Homeobox 2A; Homeobox B5; Homeobox protein HHO.C10; Homeobox protein Hox B5; Homeobox protein Hox-2A; Homeobox protein Hox-B5; Homeobox protein HoxB5; Homeobox protein Hu-1; Homeobox2A; HomeoboxB5; HOX 2; Hox 2.1; HOX 2A; HOX B5; HOX2; Hox2.1; Hox2.1, mouse, homolog of; HOX2A; HOXB 5; Hoxb5; HU 1; HU1; HXB5_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P09067 HXB5_HUMAN:

Spinal cord.

Description:
Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.
Sequence:
MSSYFVNSFSGRYPNGPDYQLLNYGSGSSLSGSYRDPAAMHTGSYGYNYNGMDLSVNRSSASSSHFGAVGESSRAFPAPAQEPRFRQAASSCSLSSPESLPCTNGDSHGAKPSASSPSDQATSASSSANFTEIDEASASSEPEEAASQLSSPSLARAQPEPMATSTAAPEGQTPQIFPWMRKLHISHDMTGPDGKRARTAYTRYQTLELEKEFHFNRYLTRRRRIEIAHALCLSERQIKIWFQNRRMKWKKDNKLKSMSLATAGSAFQP

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
85
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P09067 As Substrate

Site PTM Type Enzyme
S55 Phosphorylation
S186 Phosphorylation
T199 Phosphorylation
Y201 Phosphorylation
T202 Phosphorylation
K211 Ubiquitination
R217 Methylation
S257 Phosphorylation
S259 Phosphorylation

Research Backgrounds

Function:

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis.

Subcellular Location:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Spinal cord.

Family&Domains:

Belongs to the Antp homeobox family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.