CD147 Antibody - #AF5221

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Product: CD147 Antibody
Catalog: AF5221
Description: Rabbit polyclonal antibody to CD147
Application: WB IHC IF/ICC
Cited expt.: IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Sheep, Rabbit, Dog
Mol.Wt.: 42 kDa, 70 kDa; 42kD(Calculated).
Uniprot: P35613
RRID: AB_2837707

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(91%), Sheep(88%), Rabbit(89%), Dog(91%)
Clonality:
Polyclonal
Specificity:
CD147 Antibody detects endogenous levels of total CD147.
RRID:
AB_2837707
Cite Format: Affinity Biosciences Cat# AF5221, RRID:AB_2837707.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5A11 antigen; 5F7; BASI_HUMAN; Basigin (Ok blood group); Basigin; Blood brain barrier HT7 antigen; Bsg; CD 147; CD147; CD147 antigen; Collagenase stimulatory factor; EMMPRIN; Extracellular matrix metalloproteinase inducer; Leukocyte activation antigen M6; M 6; M6; M6 leukocyte activation antigen; Neurothelin; OK; OK blood group; OK blood group antigen; TCSF; Tumor cell derived collagenase stimulatory factor; Tumor cell-derived collagenase stimulatory factor;

Immunogens

Immunogen:

A synthesized peptide derived from human CD147, corresponding to a region within C-terminal amino acids.

Uniprot:

P35613(BASI_HUMAN) >>Visit HPA database.

Gene(ID):
BSG(682)
Expression:
P35613 BASI_HUMAN:

Expressed in erythrocytes (at protein level) (PubMed:28409866, PubMed:26195724). Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

Description:
Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS).
Sequence:
MAAALFVLLGFALLGTHGASGAAGFVQAPLSQQRWVGGSVELHCEAVGSPVPEIQWWFEGQGPNDTCSQLWDGARLDRVHIHATYHQHAASTISIDTLVEEDTGTYECRASNDPDRNHLTRAPRVKWVRAQAVVLVLEPGTVFTTVEDLGSKILLTCSLNDSATEVTGHRWLKGGVVLKEDALPGQKTEFKVDSDDQWGEYSCVFLPEPMGTANIQLHGPPRVKAVKSSEHINEGETAMLVCKSESVPPVTDWAWYKITDSEDKALMNGSESRFFVSSSQGRSELHIENLNMEADPGQYRCNGTSSKGSDQAIITLRVRSHLAALWPFLGIVAEVLVLVTIIFIYEKRRKPEDVLDDDDAGSAPLKSSGQHQNDKGKNVRQRNSS

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
91
Dog
91
Rabbit
89
Sheep
88
Horse
0
Bovine
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Plays an important role in targeting the monocarboxylate transporters SLC16A1, SLC16A3, SLC16A8, SLC16A11 and SLC16A12 to the plasma membrane. Plays pivotal roles in spermatogenesis, embryo implantation, neural network formation and tumor progression. Stimulates adjacent fibroblasts to produce matrix metalloproteinases (MMPS). Seems to be a receptor for oligomannosidic glycans. In vitro, promotes outgrowth of astrocytic processes.

May induce the production of pro-inflammatory cytokines such as IL6 in monocytes.

(Microbial infection) Erythrocyte receptor for P.falciparum RH5 which is essential for erythrocyte invasion by the merozoite stage of P.falciparum isolates 3D7, Dd2, 7G8 and HB3. Binding of P.falciparum RH5 results in BSG dimerization which triggers an increase in intracellular Ca(2+) in the erythrocyte. This essential step leads to a rearrangement of the erythrocyte cytoskeleton required for the merozoite invasion.

PTMs:

N-glycosylated.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In spermatozoa, localized on the principal piece of caput and in the middle piece during transit in the corpus and cauda epididymides (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in erythrocytes (at protein level). Present only in vascular endothelium in non-neoplastic regions of the brain, whereas it is present in tumor cells but not in proliferating blood vessels in malignant gliomas.

References

1). Comprehensive single-cell RNA analysis reveals intertumoral microenvironment heterogeneity and hub niche of carcinogenesis in thyroid cancer. NPJ precision oncology, 2025 (PubMed: 41298873) [IF=7.9]

Application: IF/ICC    Species: human    Sample:

Fig. 6: Cell-to-cell communications among thyrocyte and immune cells predicted by the CellChat software in PTC and Metastase. A Circle plot summarizing the maximum number of interactions among individual cell types in metastatic lesions. The thickness of the connecting lines represents the interaction strength. B Comparison of the overall information flow, including the number and strength of interactions, within the inferred networks between PTC and metastatic lesions. C Heatmap displaying the potential outgoing and incoming interaction strength of each cellular cluster in PTC and metastatic lesions. D Heatmap illustrating the relative signaling contribution of each cell group based on the number and strength of interactions, comparing PTC with metastatic lesions. E Differential network centrality analysis based on EMT-like cancer-associated fibroblasts (CAFs), comparing PTC and metastatic lesions. F Bubble heatmap showing the cell-cell communication of selected ligand-receptor pairs between EMT-like CAFs and CD8 + PDCD1 + T cells. Dot size indicates the P-value, while color represents the communication probability. G Multiplex immunohistochemistry (mIHC) validation of the cross-talk between SAA1+ CAFs and T cells via the PPIA-BSG ligand-receptor interaction.
2). Phillyrin from Forsythia suspensa suppresses the proliferation, angiogenesis, and metastasis of colorectal cancer via targeting CD147. Journal of ethnopharmacology, 2025 (PubMed: 40210179) [IF=4.8]
3). Investigation of the biomarkers and mechanism based on endoplasmic reticulum stress in irritable bowel syndrome. Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology, 2025 (PubMed: 40987697) [IF=1.1]

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