CD83 Antibody - #AF5233

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Product: CD83 Antibody
Catalog: AF5233
Description: Rabbit polyclonal antibody to CD83
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig
Mol.Wt.: 23 kDa; 23kD(Calculated).
Uniprot: Q01151
RRID: AB_2837719

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 100ul $280 In stock
 200ul $350 In stock

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(88%)
Clonality:
Polyclonal
Specificity:
CD83 Antibody detects endogenous levels of total CD83.
RRID:
AB_2837719
Cite Format: Affinity Biosciences Cat# AF5233, RRID:AB_2837719.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

B cell activation 45kDa cell surface glycoprotein Ig superfamily; B cell activation protein; B-cell activation protein; BL11; BL11 PEN; CD83; CD83 antigen (activated B lymphocytes, immunoglobulin superfamily); CD83 antigen; CD83 molecule; CD83_HUMAN; Cell surface glycoprotein; Cell surface protein HB15; HB15; hCD83;

Immunogens

Immunogen:
Uniprot:

Q01151(CD83_HUMAN) >>Visit HPA database.

Gene(ID):
CD83(9308)
Expression:
Q01151 CD83_HUMAN:

Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.

Description:
May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.
Sequence:
MSRGLQLLLLSCAYSLAPATPEVKVACSEDVDLPCTAPWDPQVPYTVSWVKLLEGGEERMETPQEDHLRGQHYHQKGQNGSFDAPNERPYSLKIRNTTSCNSGTYRCTLQDPDGQRNLSGKVILRVTGCPAQRKEETFKKYRAEIVLLLALVIFYLTLIIFTCKFARLQSIFPDFSKAGMERAFLPVTSPNKHLGLVTPHKTELV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
88
Horse
67
Rabbit
63
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q01151 As Substrate

Site PTM Type Enzyme
K76 Ubiquitination
N79 N-Glycosylation
S99 Phosphorylation
T104 Phosphorylation
Y105 Phosphorylation
T188 Phosphorylation
S189 Phosphorylation
K192 Ubiquitination
T198 Phosphorylation
K201 Ubiquitination

Research Backgrounds

Function:

May play a significant role in antigen presentation or the cellular interactions that follow lymphocyte activation.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed by activated lymphocytes, Langerhans cells and interdigitating reticulum cells.

Subunit Structure:

Monomer.

References

1). Targeted Covalent Nanodrugs Reinvigorate Antitumor Immunity and Kill Tumors via Improving Intratumoral Accumulation and Retention of Doxorubicin. ACS Nano, 2024 [IF=15.8]
2). A Multifunctional Nanoparticle for Multimodal Imaging-Guided LIFU/Immuno-Synergistic Retinoblastoma Therapy. ACS Applied Materials & Interfaces, 2020 (PubMed: 31940169) [IF=8.3]

Application: WB    Species: Human    Sample: Y79 cells

Figure 12. (A,B) NOD2 and CD83 contents in Y79 tumors after different treatments. (C) IFN-γ and MCP-1 levels in Y79 tumors after different treatments. (D) H&E staining in major organs (heart, liver, spleen, lung, and kidney) of all groups after treatments. The scale bars are 50 μm. (n = 5, **p < 0.01).
3). Stromal and tumor immune microenvironment reprogramming through multifunctional cisplatin-based liposomes boosts the efficacy of anti-PD-1 immunotherapy in pancreatic cancer. Biomaterials science, 2023 (PubMed: 37921708) [IF=5.8]
4). Иммуногистохимическая характеристика биопсийных образцов десны в области беззубого альвеолярного края челюсти. Пародонтология, 2024

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