hnRNP H Antibody - #AF5241
Product: | hnRNP H Antibody |
Catalog: | AF5241 |
Description: | Rabbit polyclonal antibody to hnRNP H |
Application: | WB IHC IF/ICC |
Reactivity: | Human, Mouse, Rat |
Prediction: | Pig, Bovine, Horse, Sheep, Rabbit, Dog |
Mol.Wt.: | 49 kDa; 49kD(Calculated). |
Uniprot: | P31943 |
RRID: | AB_2837727 |
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Protocols
Product Info
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Cite Format: Affinity Biosciences Cat# AF5241, RRID:AB_2837727.
Fold/Unfold
DKFZp686A15170; Heterogeneous nuclear ribonucleoprotein H; Heterogeneous nuclear ribonucleoprotein H1 (H); Heterogeneous nuclear ribonucleoprotein H1; HNRH1_HUMAN; hnRNP H; hnRNPH; Hnrnph1; HNRPH 1; HNRPH; HNRPH1 protein; N-terminally processed;
Immunogens
- P31943 HNRH1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MMLGTEGGEGFVVKVRGLPWSCSADEVQRFFSDCKIQNGAQGIRFIYTREGRPSGEAFVELESEDEVKLALKKDRETMGHRYVEVFKSNNVEMDWVLKHTGPNSPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA
Predictions
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - P31943 As Substrate
Site | PTM Type | Enzyme | Source |
---|---|---|---|
M1 | Acetylation | Uniprot | |
M2 | Acetylation | Uniprot | |
T5 | Phosphorylation | Uniprot | |
K14 | Sumoylation | Uniprot | |
K14 | Ubiquitination | Uniprot | |
S21 | Phosphorylation | Uniprot | |
S23 | Phosphorylation | Uniprot | |
R29 | Methylation | Uniprot | |
C34 | S-Nitrosylation | Uniprot | |
K35 | Sumoylation | Uniprot | |
K35 | Ubiquitination | Uniprot | |
R44 | Methylation | Uniprot | |
Y47 | Phosphorylation | Uniprot | |
S54 | Phosphorylation | Uniprot | |
S63 | Phosphorylation | Uniprot | |
K68 | Sumoylation | Uniprot | |
K72 | Sumoylation | Uniprot | |
R81 | Methylation | Uniprot | |
K87 | Sumoylation | Uniprot | |
K87 | Ubiquitination | Uniprot | |
S88 | Phosphorylation | Uniprot | |
K98 | Acetylation | Uniprot | |
K98 | Sumoylation | Uniprot | |
K98 | Ubiquitination | Uniprot | |
T100 | Phosphorylation | Uniprot | |
S104 | Phosphorylation | P28482 (MAPK1) , P24941 (CDK2) | Uniprot |
T107 | Phosphorylation | Uniprot | |
R114 | Methylation | Uniprot | |
C122 | S-Nitrosylation | Uniprot | |
S123 | Phosphorylation | Uniprot | |
S151 | Phosphorylation | Uniprot | |
T152 | O-Glycosylation | Uniprot | |
S161 | Phosphorylation | Uniprot | |
K167 | Acetylation | Uniprot | |
K167 | Ubiquitination | Uniprot | |
K170 | Ubiquitination | Uniprot | |
K171 | Ubiquitination | Uniprot | |
Y180 | Phosphorylation | Uniprot | |
K185 | Acetylation | Uniprot | |
K185 | Methylation | Uniprot | |
K185 | Sumoylation | Uniprot | |
K185 | Ubiquitination | Uniprot | |
S186 | Phosphorylation | Uniprot | |
S187 | Phosphorylation | Uniprot | |
T193 | Phosphorylation | Uniprot | |
Y195 | Phosphorylation | Uniprot | |
R199 | Methylation | Uniprot | |
K200 | Ubiquitination | Uniprot | |
R206 | Methylation | Uniprot | |
Y210 | Phosphorylation | Uniprot | |
R212 | Methylation | Uniprot | |
R217 | Methylation | Uniprot | |
Y219 | Phosphorylation | Uniprot | |
S221 | Phosphorylation | Uniprot | |
R224 | Methylation | Uniprot | |
R230 | Methylation | Uniprot | |
R232 | Methylation | Uniprot | |
R233 | Methylation | Uniprot | |
Y236 | Phosphorylation | Uniprot | |
Y240 | Phosphorylation | Uniprot | |
Y243 | Phosphorylation | Uniprot | |
Y246 | Phosphorylation | Uniprot | |
Y249 | Phosphorylation | Uniprot | |
Y253 | Phosphorylation | Uniprot | |
S257 | Phosphorylation | Uniprot | |
R259 | Methylation | Uniprot | |
R262 | Methylation | Uniprot | |
Y266 | Phosphorylation | Uniprot | |
C267 | S-Nitrosylation | Uniprot | |
S269 | Phosphorylation | Uniprot | |
S272 | Phosphorylation | Uniprot | |
Y276 | Phosphorylation | Uniprot | |
T286 | Phosphorylation | Uniprot | |
R294 | Methylation | Uniprot | |
T301 | Phosphorylation | Uniprot | |
Y306 | Phosphorylation | Uniprot | |
S310 | Phosphorylation | Uniprot | |
T328 | Phosphorylation | Uniprot | |
S346 | Phosphorylation | Uniprot | |
K349 | Ubiquitination | Uniprot | |
S368 | Phosphorylation | Uniprot | |
Y372 | Phosphorylation | Uniprot | |
Y376 | Phosphorylation | Uniprot |
Research Backgrounds
This protein is a component of the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. Mediates pre-mRNA alternative splicing regulation. Inhibits, together with CUGBP1, insulin receptor (IR) pre-mRNA exon 11 inclusion in myoblast. Binds to the IR RNA. Binds poly(RG).
Nucleus>Nucleoplasm.
Expressed ubiquitously.
Part of a ternary complex containing FUBP2, PTBP1, PTBP2 and HNRNPH1. Identified in the spliceosome C complex. Interacts with IGF2BP1. Interacts with CUGBP1; the interaction is RNA-dependent. Interacts with MBNL1; the interaction in RNA-independent.
Each quasi-RRM repeat bound poly(RG), while only the N-terminal QRRM bound poly(RC) and poly(RU). None of the repeats bound detectable amounts of poly(RA).
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