EPHB2 Antibody - #AF5246

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Product: EPHB2 Antibody
Catalog: AF5246
Description: Rabbit polyclonal antibody to EPHB2
Application: WB IHC IF/ICC
Cited expt.: WB
Reactivity: Human, Mouse, Rat
Mol.Wt.: 117 kDa; 117kD(Calculated).
Uniprot: P29323
RRID: AB_2837732

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
EPHB2 Antibody detects endogenous levels of total EPHB2.
RRID:
AB_2837732
Cite Format: Affinity Biosciences Cat# AF5246, RRID:AB_2837732.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

cek5; Developmentally regulated EPH related tyrosine kinase; DRT; EK5; ELK related protein tyrosine kinase; Eph receptor B2; EPH tyrosine kinase 3; EPH-like kinase 5; EPHB2; EPHB2_HUMAN; Ephrin type B receptor 2; Ephrin type-B receptor 2; EPHT 3; ERK; ETECK; hEK5; Nuk; Prkm 5; Receptor protein tyrosine kinase HEK 5; Renal carcinoma antigen NY-REN-47; Sek 3; Tyro 5; Tyrosine protein kinase receptor CEK 5; Tyrosine protein kinase receptor EPH 3; Tyrosine protein kinase receptor QEK 5; Tyrosine-protein kinase receptor EPH-3; Tyrosine-protein kinase TYRO5;

Immunogens

Immunogen:

A synthesized peptide derived from human EPHB2, corresponding to a region within C-terminal amino acids.

Uniprot:

P29323(EPHB2_HUMAN) >>Visit HPA database.

Gene(ID):
EPHB2(2048)
Expression:
P29323 EPHB2_HUMAN:

Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

Description:
Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling.
Sequence:
MALRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDSATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRSGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIQEKLPLIIGSSAAGLVFLIAVVVIAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAGEFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITEFMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRFLEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIEQDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIPDYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQIQSVEGQPLARRPRATGRTKRCQPRDVTKKTCNSNDGKKKGMGKKKTDPGRGREIQGIFFKEDSHKESNDCSCGG

Research Backgrounds

Function:

Receptor tyrosine kinase which binds promiscuously transmembrane ephrin-B family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Functions in axon guidance during development. Involved in the guidance of commissural axons, that form a major interhemispheric connection between the 2 temporal lobes of the cerebral cortex. Also involved in guidance of contralateral inner ear efferent growth cones at the midline and of retinal ganglion cell axons to the optic disk. In addition to axon guidance, also regulates dendritic spines development and maturation and stimulates the formation of excitatory synapses. Upon activation by EFNB1, abolishes the ARHGEF15-mediated negative regulation on excitatory synapse formation. Controls other aspects of development including angiogenesis, palate development and in inner ear development through regulation of endolymph production. Forward and reverse signaling through the EFNB2/EPHB2 complex regulate movement and adhesion of cells that tubularize the urethra and septate the cloaca. May function as a tumor suppressor. May be involved in the regulation of platelet activation and blood coagulation.

PTMs:

Autophosphorylated; ligand binding stimulates autophosphorylation on tyrosine residues.

Polyubiquitinated; ligand binding stimulates ubiquitination.

Ligand binding induces cleavage by matrix metalloproteinases (MMPs) such as MMP7/MMP9, producing an EphB2/N-terminal fragment (NTF) and a C-terminal long fragment (EphB2-LF). EphB2-LF is further cleaved by MMPs, producing EphB2/CTF1 which is further cleaved by the PS1/gamma-secretase producing EphB2/CTF2.

Subcellular Location:

Cell membrane>Single-pass type I membrane protein. Cell projection>Axon. Cell projection>Dendrite.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. Preferentially expressed in fetal brain.

Family&Domains:

Belongs to the protein kinase superfamily. Tyr protein kinase family. Ephrin receptor subfamily.

Research Fields

· Organismal Systems > Development > Axon guidance.   (View pathway)

References

1). EphrinB2 overexpression enhances osteogenic differentiation of dental pulp stem cells partially through ephrinB2-mediated reverse signaling. Stem Cell Research & Therapy, 2020 (PubMed: 31996240) [IF=7.5]

Application: WB    Species: Human    Sample: hDPSCs

Fig. 4 EphrinB2 overexpression promoted osteogenic differentiation of hDPSCs partially via enhancement of ephrinB2-mediated reverse signaling. a Overexpression of ephrinB2 elevated p-ephrinB2 but did not affect p-EphB4 and RhoA activity in hDPSCs during osteogenic differentiation. b Western blot analysis confirmed that ephrinB2 reverse signaling was activated by 1 μg/ml EphB4-Fc. c Alizarin Red S staining of hDPSCs treated with EphB4-Fc at different concentrations (0, 2, and 4 μg/ml) in osteogenic medium for 14 days. Scale bar = 200 μm. d Transcription of RUNX2, BMP2, and OCN in EphB4-Fc-treated hDPSCs on day 7 of osteogenic induction. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 and **p < 0.01

Application: WB    Species: human    Sample: hDPSCs

Figure S1.| Expression patterns of EphB1, EphB2, and EphB4 in ephrinB2-overexpressing hDPSCs. (A) EphB4 mRNA expression level was lower in ephrinB2-overexpressing hDPSCs on days 0 and 7 of osteogenic induction. (B) EphB4 protein expression level was lower in ephrinB2-overexpressing hDPSCs on days 0 and 7 of osteogenic induction. (C) Overexpression of ephrinB2 did not affect EphB1 and EphB2 protein expression levels. Data are shown as mean ± SD. Assays were repeated three times. *p < 0.05 and **p < 0.01 (TIF 10113 kb)
2). Activation of ephrinb1/EPHB2/MAP-2/NMDAR Mediates Hippocampal Neurogenesis Promoted by Transcranial Direct Current Stimulation in Cerebral-Ischemic Mice. NEUROMOLECULAR MEDICINE, 2021 (PubMed: 33782855) [IF=3.3]

Application: WB    Species: Mice    Sample: hippocampal

Fig. 5 tDCS upregulate expression level of EPHB2, ephrinb1, MAP-2, NMDAR in the hippocampal of CI mice. a The values of mRNA expression. b Results are immunoblots from single representative experiment. c The values of protein expression. The data are expressed as the mean±SD (n=4). *P<0.05, **P<0.01 compared with Sham group, # P<0.05, ##P<0.01 compared with Model group

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