LEG7 Antibody - #AF5300

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Product: LEG7 Antibody
Catalog: AF5300
Description: Rabbit polyclonal antibody to LEG7
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 15 KD; 15kD(Calculated).
Uniprot: P47929
RRID: AB_2837785

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
LEG7 Antibody detects endogenous levels of total LEG7.
RRID:
AB_2837785
Cite Format: Affinity Biosciences Cat# AF5300, RRID:AB_2837785.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

Gal-7; GAL7; Galectin 7B; Galectin-7; HKL-14; Human keratinocyte lectin 14; Keratinocyte lectin 14; Lectin galactoside binding soluble 7; Lectin, galactoside binding, soluble, 7B; LEG7_HUMAN; LGALS7; LGALS7A; LGALS7B; P53 induced protein 1; p53-induced gene 1 protein; Pi7; PIG1; TP53I1;

Immunogens

Immunogen:
Uniprot:

P47929(LEG7_HUMAN) >>Visit HPA database.

Gene(ID):
LGALS7; LGALS7B(3963)
Expression:
P47929 LEG7_HUMAN:

Mainly expressed in stratified squamous epithelium.

Description:
Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.
Sequence:
MSNVPHKSSLPEGIRPGTVLRIRGLVPPNASRFHVNLLCGEEQGSDAALHFNPRLDTSEVVFNSKEQGSWGREERGPGVPFQRGQPFEVLIIASDDGFKAVVGDAQYHHFRHRLPLARVRLVEVGGDVQLDSVRIF

PTMs - P47929 As Substrate

Site PTM Type Enzyme
S9 Phosphorylation
T18 Phosphorylation
S69 Phosphorylation
Y107 Phosphorylation

Research Backgrounds

Function:

Could be involved in cell-cell and/or cell-matrix interactions necessary for normal growth control. Pro-apoptotic protein that functions intracellularly upstream of JNK activation and cytochrome c release.

Subcellular Location:

Cytoplasm. Nucleus. Secreted.
Note: May be secreted by a non-classical secretory pathway.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Mainly expressed in stratified squamous epithelium.

Subunit Structure:

Monomer.

References

1). CRLF1 is a key regulator in the ligamentum flavum hypertrophy. Frontiers in Cell and Developmental Biology, 2020 (PubMed: 33072735) [IF=4.6]

Application: WB    Species: human    Sample: LF cells

FIGURE 6 | CRLF1 plays a crucial role in LF fibrosis. (A) CRLF1 expression induced by TGF-β1 in LF cells transfected with siRNAs. (B,C) ERK signaling pathway activation by CRLF1 and TGF-β1. (D) siCRLF1 attenuated the ERK phosphorylation induced by TGF-β1. (E) siCRLF1 reduced the fibrosis markers expression induced by TGF-β1. (F,G) Inhibition of the ERK pathway attenuated the CRLF1- and TGF-β1-induced expressions of fibrosis markers in LF cells. (H) siCRLF1 significantly reduced the pro-fibrotic effect of IL-1β (20 ng/ml). (I) siCRLF1 significantly reduced the pro-fibrotic effect of mechanical stretching forces.

Application: WB    Species: human    Sample: LF cells

FIGURE 4 | CRLF1 stimulates the cell matrix at the post-transcriptional level. LF cells were exposed to CRLF1 for 24 h. (A) Cell lysates were obtained for Western blot analysis.

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For Research Use Only.
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