AGER/RAGE Antibody - #AF5309

Fig. 4: Single-Cell transcriptional analysis unravels heterogeneity of fibroblasts during FHP.A t-SNE visualization of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts. B The heatmap showing the expression levels of top five marker genes in fibroblast subpopulations. C Relative percentage of fibroblast subpopulations in lung from the patient with FHP. D Violin plots displaying the expression levels of the representative genes in each fibroblast subpopulation. E Relative gene set enrichment scores of subpopulations calculated by AUCell analysis. F Multiplex immunofluorescence images of TCF21, ACTA2, and COL1A1 in lung tissues from FHP. Scale bar = 10 μm. G Trajectory analysis of PLA2G2Ahigh fibroblasts, COL1A1high fibroblasts, TCF21high fibroblasts, and ACTA2high fibroblasts using Monocle 2 (upper) and Velocyto R package (lower). H CEBPD_extended motif showed greater enrichment of regulon activity for PLA2G2Ahigh fibroblasts using SCENIC analysis (left panel). MEF2C_extended motif showed greater enrichment of regulon activity for ACTA2high fibroblasts (left panel). t-SNE visualization of AUC values of CEBPD_extended and MEF2C_extended motifs in fibroblasts (right panel). I Schematic developmental trajectories of fibroblast subpopulations in FHP lungs. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.

Fig. 2: TM reduces α-syn generation in neurons. a Representative western blotting of TM and α-syn in the PC12 cells treated with TM plasmid (TM). b Densitometry analysis of TM and α-syn in (a). n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. c Representative confocal images of TM (green) and α-syn (green) in the primary neurons (DIV10, left panel: red; right panel: yellow) treated with lentiviral carrying TM plasmid or Vector plasmid for 72 h. Scale bar represents 10 μm. d The fluorescent area of TM and α-syn in (c) was quantified by IpWin32 software. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. e The levels of α-syn in primary neurons (DIV10) treated with TM or Vector was determined by ELISA. n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. f Representative western blotting of TM and α-syn in the primary neurons treated with TM plasmid (TM). g Densitometry analysis of TM and α-syn in (f). n = 3 represents three independent experiments. Data are mean ± SEM, and an unpaired t test with two-tailed was used for statistical analysis. h The levels of TM, RAGE, t-Erk1/2, p-Erk1/2, t-c-Jun, p-c-Jun and α-syn in primary neurons with TM overexpression were analyzed by western blotting. β-actin was used as a control. i Relative levels of TM, RAGE, t-Erk1/2, p-Erk1/2, t-c-Jun, p-c-Jun and α-syn in (h) were quantified using Image J software. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. j The mRNA levels of THBD, RAGE, Erk1/2, c-Jun and SNCA (encodes α-syn) in primary neurons with TM overexpression or control were detected by qPCR. n = 3 represents three independent experiments. Data are mean ± SEM, and a one-way ANOVA followed by Tukey’s multiple comparison test. *P 

Fig. 7. Western blot was used to detect the expression levels of RAGE, PI3K, p-PI3K, AKT, p-AKT, mTOR, and p-mTOR in SCC-9 cells after treatment with BBR for 48 h, along with the corresponding bar chart. All data represent the mean ± SD, n = 3, *P 

Fig. 4 Silencing of HMGB1 relieved inflammation in vivo and vitro via RAGE/TLR4. A PPI network in HMGB 1 in inflammatory diseases in the brain. Different colors represent different molecules, and the connecting lines represent the existence of mutual relations. Shorter the connecting line, fewer nodes pass through, indicating stronger interrelationships. B HMGB1 mRNA levels in ipsilateral brain tissues in different groups. C Protein levels of HMGB1 relative to internal reference in ipsilateral brain tissue from different groups mice. D HMGB1 mRNA levels in primary cortical neurons in different groups. E The HMGB1 protein levels in primary cortical neurons in different groups. F Detection of proinflammatory factors in culture supernatant of primary neuronal cells. n = 6 in vivo experiments and n = 4 in vitro experiments. * p 

Fig. 4. |Naringin ameliorates NASH through modulation of the AGE/RAGE mechanism. The level of AGE was determined in HepG2 cells and plasma by (A) ELISA and(B) fluorimetry. Receptor for AGE (RAGE) was analyzed in HepG2 cells and liver tissue by (C) qPCR (n = 6) and (D and E) immunoblotting (n = 3). Expressions were normalized by GAPDH. RAGE expression was further validated by immunofluorescence in (F) cells, (G) its quantification (n = 5), and (H) tissue and (I) its quantification (n = 5) by using Alexa Fluor 594 (red), DAPI (blue). Magnification 40×. Data are shown as Mean ± S.E.M (n = 8), *Control vs NASH; #NASH vs NAR. **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001.

Fig. 4. |Naringin ameliorates NASH through modulation of the AGE/RAGE mechanism. The level of AGE was determined in HepG2 cells and plasma by (A) ELISA and(B) fluorimetry. Receptor for AGE (RAGE) was analyzed in HepG2 cells and liver tissue by (C) qPCR (n = 6) and (D and E) immunoblotting (n = 3). Expressions were normalized by GAPDH. RAGE expression was further validated by immunofluorescence in (F) cells, (G) its quantification (n = 5), and (H) tissue and (I) its quantification (n = 5) by using Alexa Fluor 594 (red), DAPI (blue). Magnification 40×. Data are shown as Mean ± S.E.M (n = 8), *Control vs NASH; #NASH vs NAR. **P < 0.01, ***P < 0.001; #P < 0.05, ##P < 0.01, ###P < 0.001.