Product: S100A4 Antibody
Catalog: AF5320
Description: Rabbit polyclonal antibody to S100A4
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 12 kDa; 12kD(Calculated).
Uniprot: P26447
RRID: AB_2837805

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 100ul $280 In stock
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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
Clonality:
Polyclonal
Specificity:
S100A4 Antibody detects endogenous levels of total S100A4.
RRID:
AB_2837805
Cite Format: Affinity Biosciences Cat# AF5320, RRID:AB_2837805.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

18A2; 42A; calcium Placental protein; Calvasculin; CAPL; Fibroblast specific protein 1 (FSP1); Fibroblast specific protein 1; Fibroblast specific protein; FSP1; Leukemia multidrug resistance associated protein; Malignant transformation suppression 1 (MTS1); Malignant transformation suppression 1; Metastasin; MTS1; OTTHUMP00000015467; OTTHUMP00000015468; P9KA; PEL98; Placental calcium-binding protein; Protein Mts1; Protein S100 A4; Protein S100-A4; S100 calcium binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4; S100 calcium-binding protein A4; S100a4; S10A4_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Expression:
P26447 S10A4_HUMAN:

Ubiquitously expressed.

Description:
Tissue specificityUbiquitously expressed.Sequence similaritiesBelongs to the S-100 family. Contains 2 EF-hand domains.
Sequence:
MACPLEKALDVMVSTFHKYSGKEGDKFKLNKSELKELLTRELPSFLGKRTDEAAFQKLMSNLDSNRDNEVDFQEYCVFLSCIAMMCNEFFEGFPDKQPRKK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P26447 As Substrate

Site PTM Type Enzyme
M1 Acetylation
K7 Acetylation
K18 Acetylation
K18 Ubiquitination
K22 Sumoylation
K22 Ubiquitination
K28 Sumoylation
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
S32 Phosphorylation
K35 Acetylation
K35 Ubiquitination
S44 Phosphorylation
K48 Acetylation
K48 Ubiquitination
K57 Acetylation
K57 Ubiquitination
S64 Phosphorylation
K96 Sumoylation
K96 Ubiquitination

Research Backgrounds

Tissue Specificity:

Ubiquitously expressed.

Subunit Structure:

Homodimer. Interacts with PPFIBP1 in a calcium-dependent mode.

Family&Domains:

Belongs to the S-100 family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.