Product: Ku70/80 Antibody
Catalog: AF5472
Description: Rabbit polyclonal antibody to Ku70/80
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Zebrafish
Mol.Wt.: 70, 82 kDa; 70kD,83kD(Calculated).
Uniprot: P12956 | P13010
RRID: AB_2837954

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Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Zebrafish(100%)
Clonality:
Polyclonal
Specificity:
Ku70/80 Antibody detects endogenous levels of total Ku70/80.
RRID:
AB_2837954
Cite Format: Affinity Biosciences Cat# AF5472, RRID:AB_2837954.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

5''-deoxyribose-5-phosphate lyase Ku70; 5''-dRP lyase Ku70; 70 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 1; ATP dependent DNA helicase II 70 kDa subunit; ATP-dependent DNA helicase 2 subunit 1; ATP-dependent DNA helicase II 70 kDa subunit; CTC box binding factor 75 kDa subunit; CTC box-binding factor 75 kDa subunit; CTC75; CTCBF; DNA repair protein XRCC6; G22P1; Ku 70; Ku autoantigen p70 subunit; Ku autoantigen, 70kDa; Ku p70; Ku70; Ku70 DNA binding component of DNA-dependent proteinkinase complex (thyroid autoantigen 70 kDa; Kup70; Lupus Ku autoantigen protein p70; ML8; Thyroid autoantigen 70kD (Ku antigen); Thyroid autoantigen; Thyroid lupus autoantigen; Thyroid lupus autoantigen p70; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair complementing defective repair in Chinese hamster cells 6; X-ray repair cross-complementing protein 6; XRCC 6; Xrcc6; XRCC6_HUMAN; 86 kDa subunit of Ku antigen; ATP dependent DNA helicase 2 subunit 2; ATP dependent DNA helicase II 80 kDa subunit; ATP dependent DNA helicase II 86 Kd subunit; ATP dependent DNA helicase II; ATP-dependent DNA helicase 2 subunit 2; ATP-dependent DNA helicase II 80 kDa subunit; CTC box binding factor 85 kDa; CTC box-binding factor 85 kDa subunit; CTC85; CTCBF; DNA repair protein XRCC5; KARP 1; KARP1; Ku 80; Ku autoantigen 80kDa; Ku80; Ku86; Ku86 autoantigen related protein 1; KUB 2; KUB2; Lupus Ku autoantigen protein p86; NFIV; Nuclear factor IV; Thyroid lupus autoantigen; Thyroid-lupus autoantigen; TLAA; X ray repair complementing defective repair in Chinese hamster cells 5 (double strand break rejoining); X-ray repair complementing defective repair in Chinese hamster cells 5 (double-strand-break rejoining); X-ray repair cross-complementing protein 5; Xray repair complementing defective repair in Chinese hamster cells 5; XRCC 5; XRCC5; XRCC5_HUMAN;

Immunogens

Immunogen:
Uniprot:
Gene(ID):
Description:
The Ku autoantigen is a heterodimer of 70kDa (p70) and 80kDa (p80) proteins. The p70 / p80 dimer is important for function of a 460kDa DNA-dependent protein kinase that phosphorylates certain transcription factors, including Sp1, Oct-1, p53, and SV40 large T antigen in vitro. The Ku protein plays a role in cell signaling, proliferation, DNA repair, replication, transcriptional activation, and apoptosis.
Sequence:
MSGWESYYKTEGDEEAEEEQEENLEASGDYKYSGRDSLIFLVDASKAMFESQSEDELTPFDMSIQCIQSVYISKIISSDRDLLAVVFYGTEKDKNSVNFKNIYVLQELDNPGAKRILELDQFKGQQGQKRFQDMMGHGSDYSLSEVLWVCANLFSDVQFKMSHKRIMLFTNEDNPHGNDSAKASRARTKAGDLRDTGIFLDLMHLKKPGGFDISLFYRDIISIAEDEDLRVHFEESSKLEDLLRKVRAKETRKRALSRLKLKLNKDIVISVGIYNLVQKALKPPPIKLYRETNEPVKTKTRTFNTSTGGLLLPSDTKRSQIYGSRQIILEKEETEELKRFDDPGLMLMGFKPLVLLKKHHYLRPSLFVYPEESLVIGSSTLFSALLIKCLEKEVAALCRYTPRRNIPPYFVALVPQEEELDDQKIQVTPPGFQLVFLPFADDKRKMPFTEKIMATPEQVGKMKAIVEKLRFTYRSDSFENPVLQQHFRNLEALALDLMEPEQAVDLTLPKVEAMNKRLGSLVDEFKELVYPPDYNPEGKVTKRKHDNEGSGSKRPKVEYSEEELKTHISKGTLGKFTVPMLKEACRAYGLKSGLKKQELLEALTKHFQD

MVRSGNKAAVVLCMDVGFTMSNSIPGIESPFEQAKKVITMFVQRQVFAENKDEIALVLFGTDGTDNPLSGGDQYQNITVHRHLMLPDFDLLEDIESKIQPGSQQADFLDALIVSMDVIQHETIGKKFEKRHIEIFTDLSSRFSKSQLDIIIHSLKKCDISLQFFLPFSLGKEDGSGDRGDGPFRLGGHGPSFPLKGITEQQKEGLEIVKMVMISLEGEDGLDEIYSFSESLRKLCVFKKIERHSIHWPCRLTIGSNLSIRIAAYKSILQERVKKTWTVVDAKTLKKEDIQKETVYCLNDDDETEVLKEDIIQGFRYGSDIVPFSKVDEEQMKYKSEGKCFSVLGFCKSSQVQRRFFMGNQVLKVFAARDDEAAAVALSSLIHALDDLDMVAIVRYAYDKRANPQVGVAFPHIKHNYECLVYVQLPFMEDLRQYMFSSLKNSKKYAPTEAQLNAVDALIDSMSLAKKDEKTDTLEDLFPTTKIPNPRFQRLFQCLLHRALHPREPLPPIQQHIWNMLNPPAEVTTKSQIPLSKIKTLFPLIEAKKKDQVTAQEIFQDNHEDGPTAKKLKTEQGGAHFSVSSLAEGSVTSVGSVNPAENFRVLVKQKKASFEEASNQLINHIEQFLDTNETPYFMKSIDCIRAFREEAIKFSEEQRFNNFLKALQEKVEIKQLNHFWEIVVQDGITLITKEEASGSSVTAEEAKKFLAPKDKPSGDTAAVFEEGGDVDDLLDMI

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Zebrafish
100
Pig
55
Horse
55
Bovine
55
Dog
55
Rabbit
55
Chicken
33
Sheep
0
Xenopus
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P12956/P13010 As Substrate

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
S6 Phosphorylation P78527 (PRKDC)
Y7 Phosphorylation
Y8 Phosphorylation
K9 Sumoylation
S27 Phosphorylation P78527 (PRKDC) , Q13315 (ATM)
Y30 Phosphorylation
K31 Acetylation
K31 Sumoylation
K31 Ubiquitination
S33 Phosphorylation Q13315 (ATM) , P78527 (PRKDC)
S37 Phosphorylation
S45 Phosphorylation
S51 Phosphorylation P78527 (PRKDC)
S53 Phosphorylation
K92 Acetylation
K92 Methylation
K92 Ubiquitination
K94 Ubiquitination
K100 Acetylation
K100 Ubiquitination
Y103 Phosphorylation
K114 Acetylation
K114 Methylation
K114 Ubiquitination
K123 Methylation
K123 Sumoylation
K123 Ubiquitination
K129 Ubiquitination
S155 Phosphorylation
S180 Phosphorylation
K182 Ubiquitination
K189 Ubiquitination
R194 Methylation
K206 Ubiquitination
K207 Methylation
K207 Ubiquitination
S222 Phosphorylation
R230 Methylation
S237 Phosphorylation
K238 Ubiquitination
R244 Methylation
K265 Ubiquitination
K282 Acetylation
K282 Ubiquitination
K287 Acetylation
K287 Ubiquitination
T292 Phosphorylation
K297 Sumoylation
K297 Ubiquitination
K299 Ubiquitination
R301 Methylation
T302 Phosphorylation
S306 Phosphorylation
S314 Phosphorylation
K317 Acetylation
K317 Sumoylation
K317 Ubiquitination
R318 Methylation
S324 Phosphorylation
K331 Acetylation
K331 Sumoylation
K331 Ubiquitination
K338 Acetylation
K338 Ubiquitination
K351 Ubiquitination
K357 Ubiquitination
K358 Ubiquitination
K392 Acetylation
K392 Ubiquitination
K424 Sumoylation
T428 Phosphorylation
K443 Sumoylation
K443 Ubiquitination
K445 Ubiquitination
K451 Ubiquitination
T455 Phosphorylation P24941 (CDK2)
K461 Acetylation
K461 Ubiquitination
K463 Ubiquitination
K468 Acetylation
K468 Ubiquitination
R474 Methylation
S475 Phosphorylation
S477 Phosphorylation O14757 (CHEK1)
K510 Sumoylation
K516 Ubiquitination
S520 Phosphorylation
K526 Sumoylation
K526 Ubiquitination
Y530 Phosphorylation P12931 (SRC)
Y534 Phosphorylation
K539 Acetylation
K539 Ubiquitination
T541 Phosphorylation
K542 Acetylation
K544 Acetylation
S550 Phosphorylation
K553 Acetylation
K553 Ubiquitination
K556 Acetylation
K556 Sumoylation
K556 Ubiquitination
S560 Phosphorylation
K565 Ubiquitination
K570 Acetylation
K570 Ubiquitination
K575 Acetylation
K575 Ubiquitination
T577 Phosphorylation
K582 Ubiquitination
Y588 Phosphorylation
K591 Ubiquitination
S592 Phosphorylation
K595 Ubiquitination
K596 Ubiquitination
T604 Phosphorylation
K605 Acetylation
K605 Ubiquitination
Site PTM Type Enzyme
K36 Ubiquitination
T39 Phosphorylation
K51 Sumoylation
S102 Phosphorylation
K125 Ubiquitination
R141 Methylation
K144 Acetylation
K144 Ubiquitination
S145 Phosphorylation
K155 Acetylation
K155 Ubiquitination
K156 Ubiquitination
K171 Ubiquitination
S175 Phosphorylation
S191 Phosphorylation
K195 Acetylation
K195 Sumoylation
K195 Ubiquitination
T198 Phosphorylation
K202 Ubiquitination
Y225 Phosphorylation
K233 Ubiquitination
C235 S-Nitrosylation
K238 Ubiquitination
S244 Phosphorylation
C249 S-Nitrosylation
S255 Phosphorylation
S258 Phosphorylation
K265 Acetylation
K265 Ubiquitination
K274 Ubiquitination
T277 Phosphorylation
K282 Ubiquitination
K285 Sumoylation
K285 Ubiquitination
K291 Ubiquitination
Y295 Phosphorylation
C296 S-Nitrosylation
K307 Sumoylation
K307 Ubiquitination
S318 Phosphorylation
S324 Phosphorylation
K325 Sumoylation
K325 Ubiquitination
K332 Acetylation
K332 Methylation
K332 Sumoylation
K332 Ubiquitination
K334 Methylation
K334 Ubiquitination
S335 Phosphorylation
K338 Acetylation
K338 Sumoylation
K338 Ubiquitination
C339 S-Nitrosylation
S341 Phosphorylation
C346 S-Nitrosylation
K347 Ubiquitination
K363 Acetylation
K363 Ubiquitination
S378 Phosphorylation
S379 Phosphorylation
K399 Acetylation
K399 Ubiquitination
Y416 Phosphorylation
S436 Phosphorylation
S437 Phosphorylation
K439 Ubiquitination
K443 Acetylation
K443 Sumoylation
K443 Ubiquitination
K465 Ubiquitination
K466 Acetylation
K466 Ubiquitination
K469 Ubiquitination
T472 Phosphorylation
K481 Ubiquitination
C493 S-Nitrosylation
T523 Phosphorylation
T524 Phosphorylation
K525 Ubiquitination
S531 Phosphorylation
K532 Acetylation
K532 Sumoylation
K532 Ubiquitination
K534 Acetylation
K534 Sumoylation
K534 Ubiquitination
T535 Phosphorylation
K543 Acetylation
K543 Sumoylation
K543 Ubiquitination
K544 Ubiquitination
K545 Ubiquitination
K565 Acetylation
K565 Sumoylation
K565 Ubiquitination
K566 Acetylation
K566 Ubiquitination
K568 Sumoylation
T569 Phosphorylation
S577 Phosphorylation P78527 (PRKDC)
S579 Phosphorylation
S580 Phosphorylation P78527 (PRKDC)
S585 Phosphorylation
T629 Phosphorylation
R640 Methylation
K648 Acetylation
K648 Ubiquitination
K660 Acetylation
K660 Methylation
K660 Sumoylation
K660 Ubiquitination
K665 Acetylation
K665 Ubiquitination
S692 Phosphorylation
S695 Phosphorylation
K702 Acetylation
K702 Methylation
K702 Ubiquitination
K710 Ubiquitination
S712 Phosphorylation
T715 Phosphorylation P78527 (PRKDC)

Research Backgrounds

Function:

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. Required for osteocalcin gene expression. Probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. 5'-dRP lyase activity allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

PTMs:

Phosphorylation by PRKDC may enhance helicase activity. Phosphorylation of Ser-51 does not affect DNA repair.

ADP-ribosylated by PARP3.

Subcellular Location:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. The dimer also associates with NAA15, and this complex binds to the osteocalcin promoter and activates osteocalcin expression. In addition, XRCC6 interacts with the osteoblast-specific transcription factors MSX2, RUNX2 and DLX5. Interacts with ELF3. Interacts with ATP23. The XRCC5/6 dimer associates in a DNA-dependent manner with APEX1. Binds to CDK9 isoform 2. Identified in a complex with DEAF1 and XRCC5. Interacts with DEAF1 (via the SAND domain); the interaction is direct and may be inhibited by DNA-binding. Interacts with CLU (By similarity). Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation. Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR). Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts with HMBOX1.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

Family&Domains:

Belongs to the ku70 family.

Function:

Single-stranded DNA-dependent ATP-dependent helicase. Has a role in chromosome translocation. The DNA helicase II complex binds preferentially to fork-like ends of double-stranded DNA in a cell cycle-dependent manner. It works in the 3'-5' direction. Binding to DNA may be mediated by XRCC6. Involved in DNA non-homologous end joining (NHEJ) required for double-strand break repair and V(D)J recombination. The XRCC5/6 dimer acts as regulatory subunit of the DNA-dependent protein kinase complex DNA-PK by increasing the affinity of the catalytic subunit PRKDC to DNA by 100-fold. The XRCC5/6 dimer is probably involved in stabilizing broken DNA ends and bringing them together. The assembly of the DNA-PK complex to DNA ends is required for the NHEJ ligation step. In association with NAA15, the XRCC5/6 dimer binds to the osteocalcin promoter and activates osteocalcin expression. The XRCC5/6 dimer probably also acts as a 5'-deoxyribose-5-phosphate lyase (5'-dRP lyase), by catalyzing the beta-elimination of the 5' deoxyribose-5-phosphate at an abasic site near double-strand breaks. XRCC5 probably acts as the catalytic subunit of 5'-dRP activity, and allows to 'clean' the termini of abasic sites, a class of nucleotide damage commonly associated with strand breaks, before such broken ends can be joined. The XRCC5/6 dimer together with APEX1 acts as a negative regulator of transcription. Plays a role in the regulation of DNA virus-mediated innate immune response by assembling into the HDP-RNP complex, a complex that serves as a platform for IRF3 phosphorylation and subsequent innate immune response activation through the cGAS-STING pathway.

PTMs:

ADP-ribosylated by PARP3.

Phosphorylated on serine residues. Phosphorylation by PRKDC may enhance helicase activity.

Sumoylated.

Ubiquitinated by RNF8 via 'Lys-48'-linked ubiquitination following DNA damage, leading to its degradation and removal from DNA damage sites. Ubiquitinated by RNF138, leading to remove the Ku complex from DNA breaks.

Subcellular Location:

Nucleus. Nucleus>Nucleolus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Heterodimer composed of XRCC5/Ku80 and XRCC6/Ku70. The dimer associates in a DNA-dependent manner with PRKDC to form the DNA-dependent protein kinase complex DNA-PK, and with the LIG4-XRCC4 complex to form the core of the non-homologous end joining (NHEJ) complex. Additional components of the NHEJ complex include NHEJ1/XLF and PAXX. The dimer also associates with NAA15, and this complex displays DNA binding activity towards the osteocalcin FGF response element (OCFRE). In addition, XRCC5 binds to the osteoblast-specific transcription factors MSX2 and RUNX2. Interacts with ELF3. May interact with APLF. The XRCC5/XRCC6 dimer associates in a DNA-dependent manner with APEX1. Identified in a complex with DEAF1 and XRCC6. Interacts with NR4A3; the DNA-dependent protein kinase complex DNA-PK phosphorylates and activates NR4A3 and prevents NR4A3 ubiquitinylation and degradation. Interacts with RNF138. Interacts with CYREN isoform 1 (CYREN-1) and isoform 4 (CYREN-2). Interacts (via N-terminus) with HSF1 (via N-terminus); this interaction is direct and prevents XRCC5/XRCC6 heterodimeric binding and non-homologous end joining (NHEJ) repair activities induced by ionizing radiation (IR). Interacts with DHX9; this interaction occurs in a RNA-dependent manner. Part of the HDP-RNP complex composed of at least HEXIM1, PRKDC, XRCC5, XRCC6, paraspeckle proteins (SFPQ, NONO, PSPC1, RBM14, and MATR3) and NEAT1 RNA. Interacts with ERCC6.

(Microbial infection) Interacts with human T-cell leukemia virus 1/HTLV-1 protein HBZ.

Family&Domains:

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ku80 family.

Research Fields

· Genetic Information Processing > Replication and repair > Non-homologous end-joining.

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