VCAM1 Antibody - #DF6082

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Product: VCAM1 Antibody
Catalog: DF6082
Description: Rabbit polyclonal antibody to VCAM1
Application: WB IHC IF/ICC
Cited expt.: WB, IHC, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Rabbit, Dog
Mol.Wt.: 80~120kDa; 81kD(Calculated).
Uniprot: P19320
RRID: AB_2838050

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(82%), Bovine(82%), Horse(100%), Rabbit(91%), Dog(82%)
Clonality:
Polyclonal
Specificity:
VCAM1 Antibody detects endogenous levels of total VCAM1.
RRID:
AB_2838050
Cite Format: Affinity Biosciences Cat# DF6082, RRID:AB_2838050.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CD106; CD106 Antigen; INCAM 100; INCAM-100; L1CAM; MGC99561; V-CAM 1; Vascular Cell Adhesion Molecule 1; Vascular cell adhesion protein 1; VCAM 1; VCAM-1; VCAM1; VCAM1_HUMAN;

Immunogens

Immunogen:

A synthesized peptide derived from human VCAM1, corresponding to a region within the internal amino acids.

Uniprot:

P19320(VCAM1_HUMAN) >>Visit HPA database.

Gene(ID):
VCAM1(7412)
Expression:
P19320 VCAM1_HUMAN:

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Description:
Vascular cell adhesion protein 1 (VCAM1) is important in cell-cell recognition. It has been reported to function in leukocyte-endothelial cell adhesion, interact with the beta-1 integrin VLA4 on leukocytes, and mediate both adhesion and signal transduction. There are two isoforms; Isoform 1, a single-pass type I membrane protein, and isoform 2, a lipid-anchor (GPI-anchor). VCAM1 is expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue. It is also expressed in the bone marrow (1).
Sequence:
MPGKMVVILGASNILWIMFAASQAFKIETTPESRYLAQIGDSVSLTCSTTGCESPFFSWRTQIDSPLNGKVTNEGTTSTLTMNPVSFGNEHSYLCTATCESRKLEKGIQVEIYSFPKDPEIHLSGPLEAGKPITVKCSVADVYPFDRLEIDLLKGDHLMKSQEFLEDADRKSLETKSLEVTFTPVIEDIGKVLVCRAKLHIDEMDSVPTVRQAVKELQVYISPKNTVISVNPSTKLQEGGSVTMTCSSEGLPAPEIFWSKKLDNGNLQHLSGNATLTLIAMRMEDSGIYVCEGVNLIGKNRKEVELIVQEKPFTVEISPGPRIAAQIGDSVMLTCSVMGCESPSFSWRTQIDSPLSGKVRSEGTNSTLTLSPVSFENEHSYLCTVTCGHKKLEKGIQVELYSFPRDPEIEMSGGLVNGSSVTVSCKVPSVYPLDRLEIELLKGETILENIEFLEDTDMKSLENKSLEMTFIPTIEDTGKALVCQAKLHIDDMEFEPKQRQSTQTLYVNVAPRDTTVLVSPSSILEEGSSVNMTCLSQGFPAPKILWSRQLPNGELQPLSENATLTLISTKMEDSGVYLCEGINQAGRSRKEVELIIQVTPKDIKLTAFPSESVKEGDTVIISCTCGNVPETWIILKKKAETGDTVLKSIDGAYTIRKAQLKDAGVYECESKNKVGSQLRSLTLDVQGRENNKDYFSPELLVLYFASSLIIPAIGMIIYFARKANMKGSYSLVEAQKSKV

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Rabbit
91
Pig
82
Bovine
82
Dog
82
Chicken
70
Sheep
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Function:

Important in cell-cell recognition. Appears to function in leukocyte-endothelial cell adhesion. Interacts with integrin alpha-4/beta-1 (ITGA4/ITGB1) on leukocytes, and mediates both adhesion and signal transduction. The VCAM1/ITGA4/ITGB1 interaction may play a pathophysiologic role both in immune responses and in leukocyte emigration to sites of inflammation.

PTMs:

Sialoglycoprotein.

Subcellular Location:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed on inflamed vascular endothelium, as well as on macrophage-like and dendritic cell types in both normal and inflamed tissue.

Family&Domains:

Either the first or the fourth Ig-like C2-type domain is required for VLA4-dependent cell adhesion.

Research Fields

· Environmental Information Processing > Signal transduction > NF-kappa B signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Cell adhesion molecules (CAMs).   (View pathway)

· Environmental Information Processing > Signal transduction > TNF signaling pathway.   (View pathway)

· Human Diseases > Infectious diseases: Parasitic > African trypanosomiasis.

· Human Diseases > Infectious diseases: Parasitic > Malaria.

· Human Diseases > Infectious diseases: Viral > HTLV-I infection.

· Organismal Systems > Immune system > Leukocyte transendothelial migration.   (View pathway)

References

1). Artificial mesenchymal stem cell extracellular vesicles enhanced ischemic stroke treatment through targeted remodeling brain microvascular endothelial cells. Acta pharmaceutica Sinica. B, 2025 (PubMed: 40893673) [IF=14.7]
2). A novel UV-curable extravascular stent to prevent restenosis of venous grafts. Composites Part B: Engineering, 2021 [IF=12.7]

Application: WB    Species: Rat    Sample:

Fig. 6. A: PCNA immunofluorescence staining of venous graft vessels in rats. B, C: Western blot analysis of α-SMA, PCNA, ICAM-1, VCAM-1, and vimentin in rat vein graft vessels. D: ICAM-1 immunofluorescence staining of venous graft vessels in rats. E: qPCR of α-SMA, PCNA, ICAM-1, VCAM-1, and vimentin in rat vein graft vessels. Data are presented as the mean ± SD; *: compared with control group, p < 0.05. #: compared with sham group,
3). Inflammation-targeted nanoparticles modulate macrophage polarization for coronary therapy in Kawasaki disease. Journal of controlled release : official journal of the Controlled Release Society, 2025 (PubMed: 41135700) [IF=10.5]
4). Qingqi Liangying formula inhibits brain endothelial cell pyroptosis via NLRP3/caspase-1/GSDMD in sepsis-associated encephalopathy. PHYTOMEDICINE, 2025 [IF=8.3]

Application: WB    Species: Mouse    Sample:

Fig. 3. The QL formula alleviates cerebral microcirculatory disorders in an LPS-induced mouse model of SAE. (A) Representative images of leukocyte-endothelium interaction in the brain microcirculation. Scale bar: 150 μm (n = 3). (B) Statistical analysis of the number of leukocytes adhering to cerebral veins in each group (n = 3). (C) Levels of VCAM-1 in the brain was detected by IHC staining (n = 5). Scale bar: 50 μm (upper image), 20 μm (lower image). (D) Protein levels of VCAM-1 and ICAM-1 were detected by Western blot, with β-Actin used as a loading control (n = 5). (E, F) Western blot bands for VCAM-1 and ICAM-1 were analyzed semi-quantitatively using ImageJ software. The relative levels of these proteins were calculated based on gray values and normalized to β-Actin (n = 5). Data are expressed as mean ± SD.
5). Novel Strategy for Isolation of Mice Bone Marrow–Derived Endothelial Cells (BMECs). Stem Cell Research & Therapy, 2020 (PubMed: 33941266) [IF=7.5]

Application: WB    Species: mouse    Sample: primary bone marrow endothelial cells

Fig. 4 |Characterization of primary bone marrow endothelial cells by RT-QPCR and immunoblottinge .The immunoblotting analysis of primary bone marrow endothelial after 7 days of cultured further substantiates the purity of endothelial cells with protein ladder (M) (N= 3 indicates independent experiments for RT-qPCR and N=2 for immunoblotting with six mice/group(12 femora and 12 tibias). *p<0.05 **p<0.01, and ***p<0.001

Application: WB    Species: Mice    Sample: bMECs

Fig. 4 Characterization of primary bone marrow endothelial cells by RT-QPCR and immunoblotting: a–d the relative mRNA expression of primary bone marrow endothelial cells compared to CD31 microbead-negative selected cells. The bar chart indicated the fold of the gene expression of primary BMECs compared to the CD31 microbead-negative selected cells. The fold change is quantified by the Pitfall method, followed by a Student’s test comparison between the CD31 microbead-negative selected cells and endothelial cells. P value ≤0.05 considered being statistically significant. e The immunoblotting analysis of primary bone marrow endothelial after 7 days of cultured further substantiates the purity of endothelial cells with protein ladder (M) (N= 3 indicates independent experiments for RT-qPCR and N=2 for immunoblotting with six mice/group (12 femora and 12 tibias). *p<0.05 **p<0.01, and ***p<0.001
6). Activation of vascular endothelial cells by synovial fibrosis promotes Netrin-1-induced sensory nerve sprouting and exacerbates pain sensitivity. Journal of cellular and molecular medicine, 2023 (PubMed: 37702437) [IF=5.3]

Application: WB    Species: Rat    Sample:

FIGURE 1 KOA synovial fibrosis activates vascular endothelial cells to promote Netrin-1 secretion. (A) Each group was stained with Sirius Red stain and Masson stain for rat synovial tissue, 200× scale bar = 50 μm, showing collagen fibre content. Each group was stained with synovial tissue using silver plated nerve, 200x scale bar = 50 μm, showing neuronal and nerve fibre content. (B) Each group used Netrin-1 + CD31 immunofluorescence staining of synovial tissue, 100× scale bar = 100 μm, to show the expression of the vascular endothelial cell marker CD31 and the expression of Netrin-1 in synovial tissue. (C–E) WB and PCR were performed to detect the protein and gene expression of Netrin-1 and vascular endothelial cell activation markers ICAM-1 and VCAM-1 in the synovial tissues of rats in each group. (*p 
7). Traditional Chinese Medicine Shi-Bi-Man ameliorates psoriasis via inhibiting IL-23/Th17 axis and CXCL16-mediated endothelial activation. Chinese medicine, 2024 (PubMed: 38429819) [IF=5.3]
8). HIF-1α depletion regulates autophagy to improve arteriosclerosis obliterans of the lower extremities via the TSP-1/CD47 axis. Life sciences, 2025 (PubMed: 40543809) [IF=5.2]
9). NAG-1/GDF15 inhibits diabetic nephropathy via inhibiting AGE/RAGE-mediated inflammation signaling pathways in C57BL/6 mice and HK-2 cells. Life Sciences, 2022 (PubMed: 36367498) [IF=5.2]
10). Nepeta angustifolia attenuates responses to vascular inflammation in high glucose-induced human umbilical vein endothelial cells through heme oxygenase-1 induction. JOURNAL OF ETHNOPHARMACOLOGY, 2019 (PubMed: 30419276) [IF=4.8]
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