CDCA8 Antibody - #DF6115

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Product: CDCA8 Antibody
Catalog: DF6115
Description: Rabbit polyclonal antibody to CDCA8
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog
Mol.Wt.: 31kDa; 31kD(Calculated).
Uniprot: Q53HL2
RRID: AB_2838082

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Pig(86%), Bovine(86%), Horse(100%), Sheep(86%), Rabbit(100%), Dog(86%)
Clonality:
Polyclonal
Specificity:
CDCA8 Antibody detects endogenous levels of total CDCA8.
RRID:
AB_2838082
Cite Format: Affinity Biosciences Cat# DF6115, RRID:AB_2838082.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

BOR; BOREA_HUMAN; Borealin; CDCA8; Cell division cycle associated 8; cell division cycle associated protein 8; Cell division cycle-associated protein 8; Dasra B; Dasra-B; DasraB; FLJ10468; FLJ12042; hDasra B; hDasra-B; MESRGP; OTTHUMP00000004514; pluripotent embryonic stem cell related gene 3 protein; Pluripotent embryonic stem cell-related gene 3 protein;

Immunogens

Immunogen:
Uniprot:

Q53HL2(BOREA_HUMAN) >>Visit HPA database.

Gene(ID):
CDCA8(55143)
Description:
Borealin is a 280-amino acid protein that stabilizes the bipolar mitotic spindle. Borealin is part of a complex called Aurora B kinase that has essential regulatory roles at centromeres and the central spindle in cells undergoing mitosis. This complex makes up the enzymatic core of the chromosomal passenger complex (CPC). It is possible that the Borealin subunit directs the CPC to centromeric DNA. Deletion of Borealin delays mitotic progression and causes kinetochore-spindle to mis-attach. Deletion also causes an increase in bipolar spindles associated with ectopic asters. These extra poles, which form after chromosomes acquire their bipolar orientation, seriously disrupt chromosome allotment during anaphase. It is true that inappropriate chromosomal segregation and cell division may cause auneuploidy leading to cancer, so an abnormal expression of Borealin may lead to gastric cancer.
Sequence:
MAPRKGSSRVAKTNSLRRRKLASFLKDFDREVEIRIKQIESDRQNLLKEVDNLYNIEILRLPKALREMNWLDYFALGGNKQALEEAATADLDITEINKLTAEAIQTPLKSAKTRKVIQVDEMIVEEEEEEENERKNLQTARVKRCPPSKKRTQSIQGKGKGKRSSRANTVTPAVGRLEVSMVKPTPGLTPRFDSRVFKTPGLRTPAAGERIYNISGNGSPLADSKEIFLTVPVGGGESLRLLASDLQRHSIAQLDPEALGNIKKLSNRLAQICSSIRTHK

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Horse
100
Pig
86
Bovine
86
Sheep
86
Dog
86
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q53HL2 As Substrate

Site PTM Type Enzyme
Sumoylation
K5 Acetylation
K12 Ubiquitination
T13 Phosphorylation
S15 Phosphorylation
K20 Ubiquitination
S23 Phosphorylation
K37 Ubiquitination
K48 Ubiquitination
T88 Phosphorylation P33981 (TTK)
T94 Phosphorylation P33981 (TTK)
K98 Ubiquitination
T100 Phosphorylation
T106 Phosphorylation
K109 Ubiquitination
S110 Phosphorylation
K112 Ubiquitination
K115 Ubiquitination
K135 Ubiquitination
T152 Phosphorylation
S154 Phosphorylation Q96GD4 (AURKB)
S164 Phosphorylation
S165 Phosphorylation Q96GD4 (AURKB)
T169 Phosphorylation P33981 (TTK)
T171 Phosphorylation
S180 Phosphorylation
K183 Ubiquitination
T185 Phosphorylation
T189 Phosphorylation
S194 Phosphorylation
K198 Ubiquitination
T199 Phosphorylation
T204 Phosphorylation
S215 Phosphorylation
S219 Phosphorylation Q96GD4 (AURKB)
S224 Phosphorylation
K225 Ubiquitination
T230 Phosphorylation P33981 (TTK)
S238 Phosphorylation P33981 (TTK)
S244 Phosphorylation
S250 Phosphorylation
K263 Ubiquitination
S274 Phosphorylation
S275 Phosphorylation Q96GD4 (AURKB)
T278 Phosphorylation Q96GD4 (AURKB)

Research Backgrounds

Function:

Component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Major effector of the TTK kinase in the control of attachment-error-correction and chromosome alignment.

PTMs:

Phosphorylated by TTK, essentially at Thr-88, Thr94, Thr-169 and Thr-230. Phosphorylation (probably by CDK1) promotes targeting of the CPC to centromeric DNA.

Sumoylated by UBE2I and RANBP2. Desumoylated by SENP3 through the removal of SUMO2 and SUMO3.

Subcellular Location:

Nucleus>Nucleolus. Cytoplasm. Cytoplasm>Cytoskeleton>Spindle. Chromosome>Centromere.
Note: Localizes on chromosome arms and inner centromeres from prophase through metaphase and then transferring to the spindle midzone and midbody from anaphase through cytokinesis. Colocalizes with SENP3 in the nucleolus in interphase cells.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

May form homooligomers and homodimers. Component of the chromosomal passenger complex (CPC) composed of at least BIRC5/survivin, CDCA8/borealin, INCENP, AURKB or AURKC; in the complex forms a triple-helix bundle-based subcomplex with INCENP and BIRC5. Interacts with SENP3, UBE2I and RANBP2. Interacts (phosphorylated) with SGO1 and SGO2; the association is dependent on CDK1.

Family&Domains:

The C-terminal region (aa 207-280) represents the dimerization motif.

Belongs to the borealin family.

References

1). CDCA8, targeted by MYBL2, promotes malignant progression and olaparib insensitivity in ovarian cancer. American Journal of Cancer Research, 2023 (PubMed: 33575078) [IF=3.6]

Application: WB    Species: human    Sample: HGSOC and FT tissues

Figure 1.| CDCA8 is key gene high expressed and correlated to chemoresistance in ovarian cancer.(S and T) The mRNA and protein levels of CDCA8 in HGSOC and FT tissues.

Application: IHC    Species: human    Sample: tumor

Figure 2.| CDCA8 promotes the proliferation and motility of ovarian cancer. (H) Representative IHC staining of Ki-67 and CDCA8 in tumor tissues (400 ×), Scale bar: 25 µm.
2). Overexpression of CDCA8 predicts poor prognosis and promotes tumor cell growth in prostate cancer. Frontiers in Oncology, 2022 (PubMed: 35449575) [IF=3.5]

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