FAIM1 Antibody - #DF6119
(1)
| Product: | FAIM1 Antibody |
| Catalog: | DF6119 |
| Description: | Rabbit polyclonal antibody to FAIM1 |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| Mol.Wt.: | 20kDa; 20kD(Calculated). |
| Uniprot: | Q9NVQ4 |
| RRID: | AB_2838086 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(88%), Dog(88%), Chicken(88%), Xenopus(88%)
Clonality:
Polyclonal
Specificity:
FAIM1 Antibody detects endogenous levels of total FAIM1.
RRID:
AB_2838086
Cite Format: Affinity Biosciences Cat# DF6119, RRID:AB_2838086.
Cite Format: Affinity Biosciences Cat# DF6119, RRID:AB_2838086.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
FAIM 1; Faim; FAIM1; FAIM1_HUMAN; Fas apoptotic inhibitory molecule 1; Fas apoptotic inhibitory molecule;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Description:
FAIM (Fas apoptosis inhibitory molecule) was identified as a protein that was inducibly expressed in B lymphocytes resistant to Fas-mediated apoptosis (1). Expression of FAIM inhibits receptor-mediated apoptosis in B cells as well as other cell types (1-3). FAIM is expressed in germinal center B cells, is positively regulated by IRF-4, and is also capable of inducing IRF-4 expression in a feed-forward mechanism (4). FAIM also regulates T cell receptor-mediated apoptosis by modulating Akt activation and Nur77 expression (2). Knockout mice for FAIM show an increased sensitivity to Fas-mediated apoptosis within B and T cells as well as hepatocytes (5). An alternatively spliced form of FAIM, termed FAIM-L, is found predominantly in the brain (6). In the nervous system, the originally identified FAIM does not appear to play a role in apoptosis, but rather can promote neurite outgrowth through the activation of Erk and NF-κB pathways (7). In contrast, FAIM-L does inhibit neuronal cell death triggered by death receptors (3).
Sequence:
- Q9NVQ4 FAIM1_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTDLVAVWDVALSDGVHKIEFEHGTTSGKRVVYVDGKEEIRKEWMFKLVGKETFYVGAAKTKATINIDAISGFAYEYTLEINGKSLKKYMEDRSKTTNTWVLHMDGENFRIVLEKDAMDVWCNGKKLETAGEFVDDGTETHFSIGNHDCYIKAVSSGKRKEGIIHTLIVDNREIPEIAS
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Horse
88
Bovine
88
Sheep
88
Dog
88
Xenopus
88
Chicken
88
Rabbit
88
Pig
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NVQ4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| T2 | Acetylation | Uniprot | |
| K29 | Ubiquitination | Uniprot | |
| K37 | Ubiquitination | Uniprot | |
| K47 | Ubiquitination | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| K60 | Ubiquitination | Uniprot | |
| K160 | Ubiquitination | Uniprot |
Research Backgrounds
Function:
Plays a role as an inducible effector molecule that mediates Fas resistance produced by surface Ig engagement in B cells.
Subcellular Location:
Cytoplasm.
Family&Domains:
Belongs to the FAIM1 family.
Restrictive clause
Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.
For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.