SNX9 Antibody - #DF6159

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Product: SNX9 Antibody
Catalog: DF6159
Description: Rabbit polyclonal antibody to SNX9
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus
Mol.Wt.: 67kDa; 67kD(Calculated).
Uniprot: Q9Y5X1
RRID: AB_2838126

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(83%), Xenopus(82%)
Clonality:
Polyclonal
Specificity:
SNX9 Antibody detects endogenous levels of total SNX9.
RRID:
AB_2838126
Cite Format: Affinity Biosciences Cat# DF6159, RRID:AB_2838126.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

MST 155; MST155; MSTP 155; MSTP155; OTTHUMP00000040083; Protein SDP 1; Protein SDP1; SDP 1; SDP1; SH3 and PX domain containing protein 3A; SH3 and PX domain containing protein SH3PX1; SH3 and PX domain-containing protein 1; SH3 and PX domain-containing protein 3A; SH3PX1; SH3PXD3A; SNX 9; SNX9; SNX9_HUMAN; Sorting nexin 9; Sorting nexin-9; WASP interactor protein; Wiskott Aldrich syndrome protein (WASP) interactor protein; Wiskott Aldrich syndrome protein interactor protein; WISP;

Immunogens

Immunogen:
Uniprot:

Q9Y5X1(SNX9_HUMAN) >>Visit HPA database.

Gene(ID):
SNX9(51429)
Expression:
Q9Y5X1 SNX9_HUMAN:

Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes.

Description:
This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but does contain a SH3 domain near its N-terminus. This protein interacts with the cytoplasmic domains of the precursor but not the processed forms of a disintegrin and metalloprotease domain 9 and 15. This protein binds the beta-appendage domain of adaptor protein 2 and may function to assist adaptor protein 2 in its role at the plasma membrane. This protein interacts with activated Cdc42-associated kinase-2 to regulate the degradation of epidermal growth factor receptor protein. [provided by RefSeq, Jul 2008]
Sequence:
MATKARVMYDFAAEPGNNELTVNEGEIITITNPDVGGGWLEGRNIKGERGLVPTDYVEILPSDGKDQFSCGNSVADQAFLDSLSASTAQASSSAASNNHQVGSGNDPWSAWSASKSGNWESSEGWGAQPEGAGAQRNTNTPNNWDTAFGHPQAYQGPATGDDDDWDEDWDGPKSSSYFKDSESADAGGAQRGNSRASSSSMKIPLNKFPGFAKPGTEQYLLAKQLAKPKEKIPIIVGDYGPMWVYPTSTFDCVVADPRKGSKMYGLKSYIEYQLTPTNTNRSVNHRYKHFDWLYERLLVKFGSAIPIPSLPDKQVTGRFEEEFIKMRMERLQAWMTRMCRHPVISESEVFQQFLNFRDEKEWKTGKRKAERDELAGVMIFSTMEPEAPDLDLVEIEQKCEAVGKFTKAMDDGVKELLTVGQEHWKRCTGPLPKEYQKIGKALQSLATVFSSSGYQGETDLNDAITEAGKTYEEIASLVAEQPKKDLHFLMECNHEYKGFLGCFPDIIGTHKGAIEKVKESDKLVATSKITLQDKQNMVKRVSIMSYALQAEMNHFHSNRIYDYNSVIRLYLEQQVQFYETIAEKLRQALSRFPVM

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
83
Xenopus
82
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y5X1 As Substrate

Site PTM Type Enzyme
Y56 Phosphorylation Q07912 (TNK2)
S116 Phosphorylation
S121 Phosphorylation
S122 Phosphorylation
T140 Phosphorylation
S174 Phosphorylation
S176 Phosphorylation
Y177 Phosphorylation
K179 Ubiquitination
S181 Phosphorylation
S183 Phosphorylation
R191 Methylation
S194 Phosphorylation
S197 Phosphorylation
S198 Phosphorylation
S199 Phosphorylation
S200 Phosphorylation
K202 Ubiquitination
K207 Ubiquitination
K213 Ubiquitination
T216 Phosphorylation
Y219 Phosphorylation
K223 Ubiquitination
Y239 Phosphorylation
S261 Phosphorylation
K267 Methylation
K267 Ubiquitination
Y272 Phosphorylation
T275 Phosphorylation
K288 Acetylation
K288 Ubiquitination
K300 Ubiquitination
S309 Phosphorylation
K313 Ubiquitination
K325 Ubiquitination
K407 Ubiquitination
K414 Acetylation
K414 Ubiquitination
K425 Ubiquitination
K433 Ubiquitination
K440 Ubiquitination
K483 Ubiquitination
K511 Ubiquitination
K528 Ubiquitination
K534 Ubiquitination
S565 Phosphorylation

Research Backgrounds

Function:

Involved in endocytosis and intracellular vesicle trafficking, both during interphase and at the end of mitosis. Required for efficient progress through mitosis and cytokinesis. Required for normal formation of the cleavage furrow at the end of mitosis. Plays a role in endocytosis via clathrin-coated pits, but also clathrin-independent, actin-dependent fluid-phase endocytosis. Plays a role in macropinocytosis. Promotes internalization of TNFR. Promotes degradation of EGFR after EGF signaling. Stimulates the GTPase activity of DNM1. Promotes DNM1 oligomerization. Promotes activation of the Arp2/3 complex by WASL, and thereby plays a role in the reorganization of the F-actin cytoskeleton. Binds to membranes enriched in phosphatidylinositol 4,5-bisphosphate and promotes membrane tubulation. Has lower affinity for membranes enriched in phosphatidylinositol 3-phosphate.

PTMs:

Ubiquitinated by ITCH.

Phosphorylated on tyrosine residues by TNK2. Phosphorylation promotes its activity in the degradation of EGFR.

Subcellular Location:

Cytoplasmic vesicle membrane>Peripheral membrane protein>Cytoplasmic side. Cell membrane>Peripheral membrane protein>Cytoplasmic side. Cytoplasmic vesicle>Clathrin-coated vesicle. Golgi apparatus>trans-Golgi network. Cell projection>Ruffle. Cytoplasm.
Note: Localized at sites of endocytosis at the cell membrane. Detected on newly formed macropinosomes. Transiently recruited to clathrin-coated pits at a late stage of clathrin-coated vesicle formation. Colocalizes with the actin cytoskeleton at the cell membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Widely expressed, with highest levels in heart and placenta, and lowest levels in thymus and peripheral blood leukocytes.

Subunit Structure:

Homodimer, and homooligomer. Heterodimer with SNX18. Interacts with ITCH. Interacts (via SH3 domain) with TNK2, WASL and ARP3. Identified in a complex with TNK2 and clathrin heavy chains. Identified in a complex with the AP-2 complex, clathrin and DNM2. Interacts (via SH3 domain) with DNM1 and DNM2. Identified in an oligomeric complex containing DNM1 and SNX9. Interacts with FCHSD1 (By similarity). Interacts with ADAM9 and ADAM15 cytoplasmic tails.

Family&Domains:

The PX domain mediates interaction with membranes enriched in phosphatidylinositol phosphate. Has high affinity for phosphatidylinositol 4,5-bisphosphate, but can also bind to membranes enriched in other phosphatidylinositol phosphates.

Belongs to the sorting nexin family.

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