SIRT7 Antibody - #DF6161

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Product: SIRT7 Antibody
Catalog: DF6161
Description: Rabbit polyclonal antibody to SIRT7
Application: WB IHC
Reactivity: Human, Mouse, Rat, Monkey
Prediction: Bovine, Dog
Mol.Wt.: 45kDa; 45kD(Calculated).
Uniprot: Q9NRC8
RRID: AB_2838128

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat,Monkey
Prediction:
Bovine(93%), Dog(93%)
Clonality:
Polyclonal
Specificity:
SIRT7 Antibody detects endogenous levels of total SIRT7.
RRID:
AB_2838128
Cite Format: Affinity Biosciences Cat# DF6161, RRID:AB_2838128.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

NAD dependent deacetylase sirtuin 7; NAD dependent protein deacetylase sirtuin 7; NAD-dependent protein deacetylase sirtuin-7; Regulatory protein SIR2 homolog 7; Silent mating type information regulation 2 homolog; Silent mating type information regulation 2 S.cerevisiae homolog 7; SIR2 L7; SIR2 like protein 7; Sir2 related protein type 7; SIR2, S.CEREVISIAE, HOMOLOG-LIKE 7; SIR2-like protein 7; SIR2L 7; SIR2L7; SIR7_HUMAN; SIRT 7; SIRT7; Sirtuin 7; Sirtuin type 7; Sirtuin7;

Immunogens

Immunogen:
Uniprot:

Q9NRC8(SIR7_HUMAN) >>Visit HPA database.

Gene(ID):
SIRT7(51547)
Description:
This gene encodes a member of the sirtuin family of proteins, homologs to the yeast Sir2 protein. Members of the sirtuin family are characterized by a sirtuin core domain and grouped into four classes. The functions of human sirtuins have not yet been determined; however, yeast sirtuin proteins are known to regulate epigenetic gene silencing and suppress recombination of rDNA. Studies suggest that the human sirtuins may function as intracellular regulatory proteins with mono-ADP-ribosyltransferase activity. The protein encoded by this gene is included in class IV of the sirtuin family. [provided by RefSeq, Jul 2008]
Sequence:
MAAGGLSRSERKAAERVRRLREEQQRERLRQVSRILRKAAAERSAEEGRLLAESADLVTELQGRSRRREGLKRRQEEVCDDPEELRGKVRELASAVRNAKYLVVYTGAGISTAASIPDYRGPNGVWTLLQKGRSVSAADLSEAEPTLTHMSITRLHEQKLVQHVVSQNCDGLHLRSGLPRTAISELHGNMYIEVCTSCVPNREYVRVFDVTERTALHRHQTGRTCHKCGTQLRDTIVHFGERGTLGQPLNWEAATEAASRADTILCLGSSLKVLKKYPRLWCMTKPPSRRPKLYIVNLQWTPKDDWAALKLHGKCDDVMRLLMAELGLEIPAYSRWQDPIFSLATPLRAGEEGSHSRKSLCRSREEAPPGDRGAPLSSAPILGGWFGRGCTKRTKRKKVT

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
93
Dog
93
Xenopus
75
Pig
0
Horse
0
Sheep
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NRC8 As Substrate

Site PTM Type Enzyme
R11 Methylation
S94 Phosphorylation
K131 Ubiquitination
T153 Phosphorylation
S359 Phosphorylation
S363 Phosphorylation
R388 Methylation

Research Backgrounds

Function:

NAD-dependent protein-lysine deacylase that can act both as a deacetylase or deacylase (desuccinylase, depropionylase and deglutarylase), depending on the context. Specifically mediates deacetylation of histone H3 at 'Lys-18' (H3K18Ac). In contrast to other histone deacetylases, displays strong preference for a specific histone mark, H3K18Ac, directly linked to control of gene expression. H3K18Ac is mainly present around the transcription start site of genes and has been linked to activation of nuclear hormone receptors; SIRT7 thereby acts as a transcription repressor. Moreover, H3K18 hypoacetylation has been reported as a marker of malignancy in various cancers and seems to maintain the transformed phenotype of cancer cells. Also able to mediate deacetylation of histone H3 at 'Lys-36' (H3K36Ac) in the context of nucleosomes. Also mediates deacetylation of non-histone proteins, such as ATM, CDK9, DDX21, DDB1, FBL, FKBP5/FKBP51, GABPB1, RAN, RRP9/U3-55K and POLR1E/PAF53. Enriched in nucleolus where it stimulates transcription activity of the RNA polymerase I complex. Acts by mediating the deacetylation of the RNA polymerase I subunit POLR1E/PAF53, thereby promoting the association of RNA polymerase I with the rDNA promoter region and coding region. In response to metabolic stress, SIRT7 is released from nucleoli leading to hyperacetylation of POLR1E/PAF53 and decreased RNA polymerase I transcription. Required to restore the transcription of ribosomal RNA (rRNA) at the exit from mitosis. Promotes pre-ribosomal RNA (pre-rRNA) cleavage at the 5'-terminal processing site by mediating deacetylation of RRP9/U3-55K, a core subunit of the U3 snoRNP complex. Mediates 'Lys-37' deacetylation of Ran, thereby regulating the nuclear export of NF-kappa-B subunit RELA/p65. Acts as a regulator of DNA damage repair by mediating deacetylation of ATM during the late stages of DNA damage response, promoting ATM dephosphorylation and deactivation. Suppresses the activity of the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complexes by mediating deacetylation of DDB1, which prevents the interaction between DDB1 and CUL4 (CUL4A or CUL4B). Activates RNA polymerase II transcription by mediating deacetylation of CDK9, thereby promoting 'Ser-2' phosphorylation of the C-terminal domain (CTD) of RNA polymerase II. Deacetylates FBL, promoting histone-glutamine methyltransferase activity of FBL. Acts as a regulator of mitochondrial function by catalyzing deacetylation of GABPB1 (By similarity). Regulates Akt/AKT1 activity by mediating deacetylation of FKBP5/FKBP51. Required to prevent R-loop-associated DNA damage and transcription-associated genomic instability by mediating deacetylation and subsequent activation of DDX21, thereby overcoming R-loop-mediated stalling of RNA polymerases. In addition to protein deacetylase activity, also acts as protein-lysine deacylase. Acts as a protein depropionylase by mediating depropionylation of Osterix (SP7), thereby regulating bone formation by osteoblasts (By similarity). Acts as a histone deglutarylase by mediating deglutarylation of histone H4 on 'Lys-91' (H4K91glu); a mark that destabilizes nucleosomes by promoting dissociation of the H2A-H2B dimers from nucleosomes. Acts as a histone desuccinylase: in response to DNA damage, recruited to DNA double-strand breaks (DSBs) and catalyzes desuccinylation of histone H3 on 'Lys-122' (H3K122succ), thereby promoting chromatin condensation and DSB repair. Also promotes DSB repair by promoting H3K18Ac deacetylation, regulating non-homologous end joining (NHEJ) (By similarity). Along with its role in DNA repair, required for chromosome synapsis during prophase I of female meiosis by catalyzing H3K18Ac deacetylation (By similarity). Involved in transcriptional repression of LINE-1 retrotransposon via H3K18Ac deacetylation, and promotes their association with the nuclear lamina. Required to stabilize ribosomal DNA (rDNA) heterochromatin and prevent cellular senescence induced by rDNA instability. Acts as a negative regulator of SIRT1 by preventing autodeacetylation of SIRT1, restricting SIRT1 deacetylase activity (By similarity).

PTMs:

Phosphorylated during mitosis.

Methylation at Arg-388 by PRMT6 inhibits the H3K18Ac histone deacetylase activity, promoting mitochondria biogenesis and maintaining mitochondria respiration.

Ubiquitinated via 'Lys-63'-linked ubiquitin chains. Deubiquitinated by USP7, inhibiting the H3K18Ac histone deacetylase activity and regulating gluconeogenesis.

Subcellular Location:

Nucleus>Nucleolus. Nucleus>Nucleoplasm. Chromosome. Cytoplasm.
Note: Mainly localizes in the nucleolus and nucleoplasm (PubMed:24207024, PubMed:28886238, PubMed:28790157, PubMed:31075303). Associated with rDNA promoter and transcribed region (PubMed:16079181, PubMed:19174463). Associated with nucleolar organizer regions during mitosis (PubMed:16079181, PubMed:19174463). In response to stress, released from nucleolus to nucleoplasm (PubMed:24207024). Associated with chromatin (PubMed:22722849). In response to DNA damage, recruited to DNA double-strand breaks (DSBs) sites (PubMed:27436229) (Probable). Located close to the nuclear membrane when in the cytoplasm (PubMed:11953824).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interacts with UBTF and the RNA polymerase I complex. Interacts with components of the B-WICH complex, such as MYBBP1A, SMARCA5/SNF2H and BAZ1B/WSTF. Interacts with ELK4, leading to stabilization at target promoters for H3K18Ac deacetylation. Interacts with histone H2A and/or histone H2B. Interacts with DNMT1 (By similarity). Interacts with SIRT1 (By similarity).

Family&Domains:

Belongs to the sirtuin family. Class IV subfamily.

References

1). Combination of resolvin E1 and lipoxin A4 promotes the resolution of pulpitis by inhibiting NF-κB activation through upregulating sirtuin 7 in dental pulp fibroblasts. CELL PROLIFERATION, 2022 (PubMed: 35411569) [IF=5.9]

Application: WB    Species: rat    Sample: Dental pulp fibroblasts

FIGURE 2 | Effects of the RvE1 and LXA4 combination on NF-κB modification.(F) Phosphorylation and acetylation levels of p65 and expressions of SIRT1, SIRT6 and SIRT7 protein (normalized to that of β-tubulin) detected by western blotting.
2). Mitochondrial glycerol 3-phosphate dehydrogenase deficiency exacerbates lipotoxic cardiomyopathy. iScience, 2024 (PubMed: 38832016) [IF=5.8]
3). H2O2 downregulate SIRT7`s protective role of endothelial premature dysfunction via microRNA-335-5p. Bioscience Reports, 2022 (PubMed: 34981113) [IF=3.8]

Application: WB    Species: Human    Sample: HUVECs

Figure 1 miR-335-5p and SIRT7 expression following treatment with high glucose, TNF-α, and H2O2 (A) Relative miR-335-5p expression in HUVECs determined using RT-PCR assay (n=3, in all groups). (B) Relative SIRT7 expression in HUVECs determined using RT-PCR assay (n=3, in all groups). (C) Western blot assay on HUVECs for SIRT7 expression following SIRT7–siRNA transfection (n=3, in all groups). NS: non-significance, **P<0.01 versus the blank or control group, ***P<0.005 versus the blank or control group, ##P<0.01 versus the underlined group, ###P<0.005 versus the underlined group. All raw Western blots data of this article are presented in Supplementary Figure S4. Values of all groups followed a Gaussian distribution.
4). SIRT7 Is a Prognostic Biomarker Associated With Immune Infiltration in Luminal Breast Cancer. Frontiers in Oncology, 2020 (PubMed: 32528869) [IF=3.5]

Application: IHC    Species: human    Sample: breast cancer

FIGURE 5 | Correlation between sirtuin (SIRT)7 expression and immune infiltration levels of M1 macrophages and T cell exhaustion in breast cancer-luminal.. (B) Tumor infiltration of M1 macrophages and T cell exhaustion in breast cancer-luminal. The expression of SIRT7 (a: + + +, d: +). The expression of IRF5 (b: ++, e: –). The expression of PD1 (PDCD1) (c: + + +, f: +). The expression density of SIRT7, PD1, and IRF5 in breast cancer tissue was quantitated by scoring staining intensity,including negative (–) and weak (+) staining, moderate (++) and strong (+ + +) staining, respectively
5). GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis. Experimental Cell Research, 2021 (PubMed: 34461110) [IF=3.3]

Application: WB    Species: Human    Sample: HCT-8 cells

Fig. 6. GRIM-19 stabilizes p53 in CRC cells through the SIRT7/PCAF/MDM2 axis. (A) Ratio of NAD + /NADH in HCT-8 cells transfected with the indicated siRNA or plasmids. (B) The half-life of MDM2 in HCT-8 cells transfected with the indicated plasmids was analyzed by Western blot. (C) Ubiquitylation levels of MDM2 in HCT-8 cells transfected with the indicated plasmids. Cells were treated with 20 μ M MG132 for 4 h before harvesting. (D) Western blot analyses of p53 expression in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (E) Ubiquitylation levels of p53 in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (F) Ubiquitylation levels of MDM2 in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (G) Acetylation levels of PCAF and MDM-2/PCAF interaction in HCT-8 cells transfected with GRIM-19 and/or siSIRT7. (H) Schematic of p53 activation by GRIM-19 through SIRT7-mediated MDM2 ubiquitination.

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