EDN3 Antibody - #DF6194
(4)
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Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
EDN3 Antibody detects endogenous levels of total EDN3.
RRID:
AB_2838160
Cite Format: Affinity Biosciences Cat# DF6194, RRID:AB_2838160.
Cite Format: Affinity Biosciences Cat# DF6194, RRID:AB_2838160.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
EDN3; EDN3_HUMAN; Endothelin-3; ET 3; ET-3; PPET3; Preproendothelin 3; Preproendothelin-3;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
P14138 EDN3_HUMAN:
Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.
Description:
The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Sequence:
- P14138 EDN3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MEPGLWLLFGLTVTSAAGFVPCSQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVEVKDQQSKQALDLHHPKLMPGSGLALAPSTCPRCLFQEGAP
PTMs - P14138 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S34 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Endothelins are endothelium-derived vasoconstrictor peptides.
Subcellular Location:
Secreted.
Tissue Specificity:
Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.
Family&Domains:
Belongs to the endothelin/sarafotoxin family.
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