EDN3 Antibody - #DF6194

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Product: EDN3 Antibody
Catalog: DF6194
Description: Rabbit polyclonal antibody to EDN3
Application: WB IHC
Reactivity: Human, Mouse, Rat
Mol.Wt.: 26kDa; 25kD(Calculated).
Uniprot: P14138
RRID: AB_2838160

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Clonality:
Polyclonal
Specificity:
EDN3 Antibody detects endogenous levels of total EDN3.
RRID:
AB_2838160
Cite Format: Affinity Biosciences Cat# DF6194, RRID:AB_2838160.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

EDN3; EDN3_HUMAN; Endothelin-3; ET 3; ET-3; PPET3; Preproendothelin 3; Preproendothelin-3;

Immunogens

Immunogen:

A synthesized peptide derived from human EDN3, corresponding to a region within C-terminal amino acids.

Uniprot:

P14138(EDN3_HUMAN) >>Visit HPA database.

Gene(ID):
EDN3(1908)
Expression:
P14138 EDN3_HUMAN:

Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.

Description:
The protein encoded by this gene is a member of the endothelin family. Endothelins are endothelium-derived vasoactive peptides involved in a variety of biological functions. The active form of this protein is a 21 amino acid peptide processed from the precursor protein. The active peptide is a ligand for endothelin receptor type B (EDNRB). The interaction of this endothelin with EDNRB is essential for development of neural crest-derived cell lineages, such as melanocytes and enteric neurons. Mutations in this gene and EDNRB have been associated with Hirschsprung disease (HSCR) and Waardenburg syndrome (WS), which are congenital disorders involving neural crest-derived cells. Four alternatively spliced transcript variants encoding three distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
Sequence:
MEPGLWLLFGLTVTSAAGFVPCSQSGDAGRRGVSQAPTAARSEGDCEETVAGPGEETVAGPGEGTVAPTALQGPSPGSPGQEQAAEGAPEHHRSRRCTCFTYKDKECVYYCHLDIIWINTPEQTVPYGLSNYRGSFRGKRSAGPLPGNLQLSHRPHLRCACVGRYDKACLHFCTQTLDVSSNSRTAEKTDKEEEGKVEVKDQQSKQALDLHHPKLMPGSGLALAPSTCPRCLFQEGAP

Research Backgrounds

Function:

Endothelins are endothelium-derived vasoconstrictor peptides.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Expressed in trophoblasts and placental stem villi vessels, but not in cultured placental smooth muscle cells.

Family&Domains:

Belongs to the endothelin/sarafotoxin family.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

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