AMBRA1 Antibody | Affinity Biosciences

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AMBRA1 Antibody - Western blot analysis of extracts from B16F10, using AMBRA1 Antibody.

Product:	AMBRA1 Antibody
Catalog:	DF6228
Description:	Rabbit polyclonal antibody to AMBRA1
Application:	WB IHC
Cited expt.:	WB
Reactivity:	Human, Mouse, Rat
Prediction:	Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Mol.Wt.:	133kDa; 143kD(Calculated).
Uniprot:	Q9C0C7
RRID:	AB_2838194

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Blocking peptides are peptides that bind specifically to the target antibody and block antibody binding. These peptide usually contains the epitope recognized by the antibody. Antibodies bound to the blocking peptide no longer bind to the epitope on the target protein. By comparing the staining from the blocked antibody versus the antibody alone, one can see which staining is specific.

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MS Report

HPLC Report

MSDS

Data Sheet

COA

Protocols

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IHC Protocol

IF/ICC Protocol

Product Info

Source:

Rabbit

Application:

WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:

Human,Mouse,Rat

Prediction:

Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(83%)

Clonality:

Polyclonal

Specificity:

AMBRA1 Antibody detects endogenous levels of total AMBRA1.

RRID:

AB_2838194
Cite Format: Affinity Biosciences Cat# DF6228, RRID:AB_2838194.

Conjugate:

Unconjugated.

Purification:

The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).

Storage:

Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

Alias:

Fold/Unfold

Activating molecule in beclin 1 regulated autophagy; Activating molecule in BECN1 regulated autophagy protein 1; Activating molecule in BECN1-regulated autophagy protein 1; Ambra1; AMRA1_HUMAN; FLJ20294; Hypothetical protein FLJ20294; Hypothetical protein LOC55626; KIAA1736; MGC33725; WDR94;

Immunogens

Immunogen:

A synthesized peptide derived from human AMBRA1, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9C0C7(AMRA1_HUMAN) >>Visit HPA database.

Gene(ID):

AMBRA1(55626)

Description:

WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids that commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms concerning chromatin assembly, cell cycle control, signal transduction, RNA processing, apoptosis and vesicular trafficking. AMBRA1 (Activating molecule in BECN1-regulated autophagy protein 1), also known as WDR94 or KIAA1736, is a 1,298 amino acid protein that contains three WD repeats. Localized to cytoplasmic vesicles, AMBRA1 functions to control protein turnover, cell proliferation and cell survival during neuronal development, thereby playing an important role in autophagy and the development of the nervous system. Multiple isoforms of AMBRA1 exist due to alternative spicing events.

Sequence:

Q9C0C7 AMRA1_HUMAN:
Protein BLAST With
NCBI/
ExPASy/
Uniprot

MKVVPEKNAVRILWGRERGARAMGAQRLLQELVEDKTRWMKWEGKRVELPDSPRSTFLLAFSPDRTLLASTHVNHNIYITEVKTGKCVHSLIGHRRTPWCVTFHPTISGLIASGCLDGEVRIWDLHGGSESWFTDSNNAIASLAFHPTAQLLLIATANEIHFWDWSRREPFAVVKTASEMERVRLVRFDPLGHYLLTAIVNPSNQQGDDEPEIPIDGTELSHYRQRALLQSQPVRRTPLLHNFLHMLSSRSSGIQVGEQSTVQDSATPSPPPPPPQPSTERPRTSAYIRLRQRVSYPTAECCQHLGILCLCSRCSGTRVPSLLPHQDSVPPASARATTPSFSFVQTEPFHPPEQASSTQQDQGLLNRPSAFSTVQSSTAGNTLRNLSLGPTRRSLGGPLSSHPSRYHREIAPGLTGSEWTRTVLSLNSRSEAESMPPPRTSASSVSLLSVLRQQEGGSQASVYTSATEGRGFPASGLATESDGGNGSSQNNSGSIRHELQCDLRRFFLEYDRLQELDQSLSGEAPQTQQAQEMLNNNIESERPGPSHQPTPHSSENNSNLSRGHLNRCRACHNLLTFNNDTLRWERTTPNYSSGEASSSWQVPSSFESVPSSGSQLPPLERTEGQTPSSSRLELSSSASPQEERTVGVAFNQETGHWERIYTQSSRSGTVSQEALHQDMPEESSEEDSLRRRLLESSLISLSRYDGAGSREHPIYPDPARLSPAAYYAQRMIQYLSRRDSIRQRSMRYQQNRLRSSTSSSSSDNQGPSVEGTDLEFEDFEDNGDRSRHRAPRNARMSAPSLGRFVPRRFLLPEYLPYAGIFHERGQPGLATHSSVNRVLAGAVIGDGQSAVASNIANTTYRLQWWDFTKFDLPEISNASVNVLVQNCKIYNDASCDISADGQLLAAFIPSSQRGFPDEGILAVYSLAPHNLGEMLYTKRFGPNAISVSLSPMGRYVMVGLASRRILLHPSTEHMVAQVFRLQQAHGGETSMRRVFNVLYPMPADQRRHVSINSARWLPEPGLGLAYGTNKGDLVICRPEALNSGVEYYWDQLNETVFTVHSNSRSSERPGTSRATWRTDRDMGLMNAIGLQPRNPATSVTSQGTQTLALQLQNAETQTEREVPEPGTAASGPGEGEGSEYGASGEDALSRIQRLMAEGGMTAVVQREQSTTMASMGGFGNNIIVSHRIHRSSQTGTEPGAAHTSSPQPSTSRGLLPEAGQLAERGLSPRTASWDQPGTPGREPTQPTLPSSSPVPIPVSLPSAEGPTLHCELTNNNHLLDGGSSRGDAAGPRGEPRNR

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species

Results

Score

Pig

100

Horse

100

Bovine

100

Sheep

100

Dog

100

Rabbit

100

Xenopus

Zebrafish

Chicken

Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Q9C0C7_AMRA1_HUMAN

Function:

Regulates autophagy and development of the nervous system. Involved in autophagy in controlling protein turnover during neuronal development, and in regulating normal cell survival and proliferation (By similarity).

Subcellular Location:

Cytoplasmic vesicle>Autophagosome.
Note: Localizes also to discrete punctae along the ciliary axoneme.

Research Fields

· Cellular Processes > Transport and catabolism > Autophagy - animal. (View pathway)

References

1). Downregulation of Ambra1 by altered DNA methylation exacerbates dopaminergic neuron damage in a fenpropathrin-induced Parkinson-like mouse model. Ecotoxicology and environmental safety, 2024 (PubMed: 38245935) [IF=6.2]

Application: WB Species: human Sample: SH-SY5Y cells

Fig. 4. Protein validation of the DNA methylation differential gene Ambra1 in vitro and in vivo. (A, B) CCK-8 assay was performed to determine the optimal concentration and time duration for Fen treatment of MN9D cells. (C, D) CCK-8 assay was established to determine the optimal concentration and time duration for Fen treatment of SH-SY5Y cells. (E, F) Representative bands and quantification of Ambra1 in Fen-induced MN9D cells. (G, H) Representative bands and quantification of Ambra1 in Fen-induced SH-SY5Y cells. (I, J) Representative bands and quantification of Ambra1 in Fen-induced primary cortical neurons. (K, L) Representative bands and quantification of Ambra1 in Fen-induced primary mesencephalic neurons. (M, N) Representative bands and quantification of Ambra1 in midbrain tissues of the control and Fen groups. In vitro experiments were performed in triplicate, n = 3 per group in vivo. Data are shown as mean ± SD. * p

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AMBRA1 Antibody - #DF6228