PDIA3 Antibody - #DF6230

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Product: PDIA3 Antibody
Catalog: DF6230
Description: Rabbit polyclonal antibody to PDIA3
Application: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
Mol.Wt.: 57kDa; 57kD(Calculated).
Uniprot: P30101
RRID: AB_2838196

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
PDIA3 Antibody detects endogenous levels of total PDIA3.
RRID:
AB_2838196
Cite Format: Affinity Biosciences Cat# DF6230, RRID:AB_2838196.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

58 kDa glucose regulated protein; 58 kDa glucose-regulated protein; 58 kDa microsomal protein; Disulfide isomerase ER 60; Disulfide isomerase ER-60; Endoplasmic reticulum resident protein 57; Endoplasmic reticulum resident protein 60; ER p57; ER protein 57; ER protein 60; ERp 57; ERp57; ERp60; ERp61; Glucose Regulated Protein 58 Kd; GRP 57; GRP 58; GRP57; HsT17083; p58; PDIA 3; PDIA3; PDIA3_HUMAN; Phospholipase C alpha; PI PLC; Protein disulfide isomerase A3; Protein disulfide isomerase family A member 3; Protein disulfide-isomerase A3;

Immunogens

Immunogen:

A synthesized peptide derived from human PDIA3, corresponding to a region within the internal amino acids.

Uniprot:

P30101(PDIA3_HUMAN) >>Visit HPA database.

Gene(ID):
PDIA3(2923)
Expression:
P30101 PDIA3_HUMAN:

Detected in the flagellum and head region of spermatozoa (at protein level).

Description:
Secretory proteins translocate into the endoplasmic reticulum (ER) after their synthesis where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Disulfide isomerase (PDI) has two thioredoxin homology domains and catalyzes the formation and isomerization of these disulfide bonds (2). Other ER resident proteins that possess the thioredoxin homology domains, including endoplasmic reticulum stress protein 57 (ERp57), constitute the PDI family (2). ERp57 interacts with calnexin and calreticulin (3) and is suggested to play a role in the isomerization of disulfide bonds on certain glycoproteins (3).
Sequence:
MRLRRLALFPGVALLLAAARLAAASDVLELTDDNFESRISDTGSAGLMLVEFFAPWCGHCKRLAPEYEAAATRLKGIVPLAKVDCTANTNTCNKYGVSGYPTLKIFRDGEEAGAYDGPRTADGIVSHLKKQAGPASVPLRTEEEFKKFISDKDASIVGFFDDSFSEAHSEFLKAASNLRDNYRFAHTNVESLVNEYDDNGEGIILFRPSHLTNKFEDKTVAYTEQKMTSGKIKKFIQENIFGICPHMTEDNKDLIQGKDLLIAYYDVDYEKNAKGSNYWRNRVMMVAKKFLDAGHKLNFAVASRKTFSHELSDFGLESTAGEIPVVAIRTAKGEKFVMQEEFSRDGKALERFLQDYFDGNLKRYLKSEPIPESNDGPVKVVVAENFDEIVNNENKDVLIEFYAPWCGHCKNLEPKYKELGEKLSKDPNIVIAKMDATANDVPSPYEVRGFPTIYFSPANKKLNPKKYEGGRELSDFISYLQREATNPPVIQEEKPKKKKKAQEDL

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Bovine
92
Sheep
92
Chicken
83
Zebrafish
75
Xenopus
67
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

Research Backgrounds

Subcellular Location:

Endoplasmic reticulum. Endoplasmic reticulum lumen. Melanosome.
Note: Identified by mass spectrometry in melanosome fractions from stage I to stage IV (PubMed:12643545).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Detected in the flagellum and head region of spermatozoa (at protein level).

Family&Domains:

Belongs to the protein disulfide isomerase family.

Research Fields

· Genetic Information Processing > Folding, sorting and degradation > Protein processing in endoplasmic reticulum.   (View pathway)

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

References

1). Novel functional mutation of the PDIA3 gene affects milk composition traits in Chinese Holstein cattle. JOURNAL OF DAIRY SCIENCE, 2022 (PubMed: 35379459) [IF=3.7]

Application: IF/ICC    Species: cow    Sample:

Figure 4. Expression and subcellular localization of PDIA3. (A) mRNA expression profile of PDIA3 in different tissues of dairy cows by quantitative real-time PCR method; (B) subcellular localization of PDIA3 in bovine mammary alveolar cells observed by immunofluorescence assay. PDIA3 (green); endoplasmic reticulum (ER) signals (red); nucleus stained with 4',6-diamidino-2-phenylindole dihydrochloride (DAPI, blue). Scale bar represents 10 μm.

Application: WB    Species: cow    Sample:

Figure 5. Detection of the efficiency of PDIA3 overexpression and knockdown in bovine mammary alveolar cells. (A) Map of PDIA3 overexpression vector (left), and agarose gel electrophoresis of pCDNA3.1- PDIA3 vector digested with restriction endonucleases (right). M = DNA marker. Lane 1 (L1) shows 2 bands of 2,248 bp and 4,671 bp with Mlu I and Xho I digestion; lane 2 (L2) shows the pCDNA3.1- PDIA3 plasmid. (B) Cells were transfected for 48 h with pCDNA3.1- PDIA3 overexpression vector (OE), pCDNA3.1 empty vector (EV), PDIA3 siRNA (KD), or negative control siRNA (NC), and the relative mRNA levels of PDIA3 were analyzed by quantitative real-time PCR. (C) Protein levels of PDIA3 were determined by western blot analysis, and quantitative results show fold increases in PDIA3/GAPDH ratios. **P < 0.01, compared with control group; ns = not significant. Error bars represent SEM. siRNA = small interfering RNA.

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