SPINK1 Antibody - #DF6239

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Product: SPINK1 Antibody
Catalog: DF6239
Description: Rabbit polyclonal antibody to SPINK1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Horse
Mol.Wt.: 9kDa; 9kD(Calculated).
Uniprot: P00995
RRID: AB_2838205

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Horse(100%)
Clonality:
Polyclonal
Specificity:
SPINK1 Antibody detects endogenous levels of total SPINK1.
RRID:
AB_2838205
Cite Format: Affinity Biosciences Cat# DF6239, RRID:AB_2838205.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

ISK1_HUMAN; Pancreatic secretory trypsin inhibitor; Pancreatic serine protease inhibitor Kazal; PCTT; PSTI; Serine protease inhibitor Kazal type 1; Serine protease inhibitor Kazal-type 1; Spink 3; SPINK1; Spink3; TaTI; TCP; Tumor associated trypsin inhibitor; Tumor-associated trypsin inhibitor;

Immunogens

Immunogen:
Uniprot:

P00995(ISK1_HUMAN) >>Visit HPA database.

Gene(ID):
SPINK1(6690)
Description:
The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]
Sequence:
MKVTGIFLLSALALLSLSGNTGADSLGREAKCYNELNGCTKIYDPVCGTDGNTYPNECVLCFENRKRQTSILIQKSGPC

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Pig
67
Bovine
67
Sheep
67
Dog
67
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00995 As Substrate

Site PTM Type Enzyme
S10 Phosphorylation
S16 Phosphorylation
S18 Phosphorylation
T69 Phosphorylation
S70 Phosphorylation

Research Backgrounds

Function:

Serine protease inhibitor which exhibits anti-trypsin activity. In the pancreas, protects against trypsin-catalyzed premature activation of zymogens (By similarity).

In the male reproductive tract, binds to sperm heads where it modulates sperm capacitance by inhibiting calcium uptake and nitrogen oxide (NO) production.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.