CYR61/CCN1 Antibody - #DF6250

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Product: CYR61/CCN1 Antibody
Catalog: DF6250
Description: Rabbit polyclonal antibody to CYR61/CCN1
Application: WB IHC IF/ICC
Reactivity: Human, Mouse, Rat
Prediction: Rabbit
Mol.Wt.: 42kDa; 42kD(Calculated).
Uniprot: O00622
RRID: AB_2838216

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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Rabbit(88%)
Clonality:
Polyclonal
Specificity:
CYR61/CCN1 Antibody detects endogenous levels of total CYR61/CCN1.
RRID:
AB_2838216
Cite Format: Affinity Biosciences Cat# DF6250, RRID:AB_2838216.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

3CH61; CCN 1; CCN family member 1; CYR 61; Cyr61; CYR61 protein; CYR61_HUMAN; Cysteine rich angiogenic inducer 61; Cysteine rich heparin binding protein 61; Cysteine-rich angiogenic inducer 61; GIG 1; GIG1; IBP-10; IGF-binding protein 10; Igfbp 10; IGFBP-10; Igfbp10; Insulin like growth factor binding protein 10; Insulin-like growth factor-binding protein 10; Protein CYR61; Protein GIG1;

Immunogens

Immunogen:
Uniprot:

O00622(CCN1_HUMAN) >>Visit HPA database.

Gene(ID):
CYR61(3491)
Description:
Cyr61 is a secreted heparin binding protein, encoded by a growth factor-inducible immediate-early gene, that associates with the extracellular matrix and connective tissue. Cyr61 is a member of a distinct family of angiogenic and vasculogenic regulators designated CCN proteins, which includes connective tissue growth factor (CTGF) and the mouse Cyr61 homolog, Fisp12. As an angiogenic inducer, Cyr61 binds to the cell surface receptor Integrin aV/b3, where it then stimulates cell adhesion and migration and promotes DNA synthesis of human vascular endothelial cells. Expression of Cyr61 is elevated during vessel growth, wound healing and chondrocyte differentiation. Cyr61 is also detected in a wide variety of tumors as it induces tumor growth and functions as a marker of tumor progression.
Sequence:
MSSRIARALALVVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPCDHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSLPNLGCPNPRLVKVTGQCCEEWVCDEDSIKDPMEDQDGLLGKELGFDASEVELTRNNELIAVGKGSSLKRLPVFGMEPRILYNPLQGQKCIVQTTSWSQCSKTCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKKGKKCSKTKKSPEPVRFTYAGCLSVKKYRPKYCGSCVDGRCCTPQLTRTVKMRFRCEDGETFSKNVMMIQSCKCNYNCPHANEAAFPFYRLFNDIHKFRD

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
88
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O00622 As Substrate

Site PTM Type Enzyme
T14 Phosphorylation
S84 Phosphorylation
T85 Phosphorylation
K88 Ubiquitination
S167 Phosphorylation
S188 Phosphorylation
K203 Ubiquitination
Y221 Phosphorylation
Y277 Phosphorylation
S278 Phosphorylation
K281 Ubiquitination

Research Backgrounds

Function:

Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5. CCN1-mediated gene regulation is dependent on heparin-binding. Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1. Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-v/beta-5 and cell proliferation through integrin alpha-v/beta-3.

Subcellular Location:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Subunit Structure:

Interaction with integrins is heparin- and cell-type-dependent and promotes cell adhesion. In skin fibroblasts it binds ITGA6/ITGB1, in endothelial cells, binds ITGAV/ITGB3 and in platelets, ITGA2B/ITGB3. Binds, in vitro, ITGAV/ITGB5.

Family&Domains:

Belongs to the CCN family.

References

1). Downregulation of miR-142-5p promotes tumor metastasis through directly regulating CYR61 expression in gastric cancer. Gastric Cancer, 2019 (PubMed: 30178386) [IF=6.0]

Application: IHC    Species: human    Sample: gastric and GC tissues

Fig. 5| High expression of CYR61 was associated with prognosis in GC. a The expression of CYR61 in normal gastric tissues (upper) and GC tissues (lower) (left: magnification ×200; right: magnification ×400).

Application: WB    Species: human    Sample: BGC823 and SGC7901 cells， AGS cells

Fig. 3 |CYR61 is a novel target gene of miR-142-5p. c Left and middle, qRT-PCR and western blot showed that the expression of CYR61 was significantly decreased in miR-142-5p-stably transfected BGC823 and SGC7901 cells as compared with control cells. Right, qRT-PCR and western blot showed that the expression of CYR61 was significantly increased in miR142-5p-inhibitor-stably transfected AGS cells as compared with control cells. *P < 0.05, **P < 0.01, ***P < 0.001.
2). Upregulation of Angiomotin-like 2 ameliorates experimental pulmonary arterial hypertension by inactivating YAP1 signaling. Journal of cardiovascular pharmacology, 2022 (PubMed: 39027975) [IF=2.6]

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