CRADD Antibody - #DF6260

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Product: CRADD Antibody
Catalog: DF6260
Description: Rabbit polyclonal antibody to CRADD
Application: WB IHC
Reactivity: Human, Mouse, Rat
Prediction: Pig, Bovine, Sheep, Dog, Chicken
Mol.Wt.: 23kDa; 23kD(Calculated).
Uniprot: P78560
RRID: AB_2838226

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 100ul $280 In stock
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Related Downloads
MS Report
HPLC Report
MSDS
Data Sheet
COA
Protocols
WB Handbook
IHC Protocol
IF/ICC Protocol

Product Info

Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

Reactivity:
Human,Mouse,Rat
Prediction:
Pig(92%), Bovine(92%), Sheep(92%), Dog(92%), Chicken(83%)
Clonality:
Polyclonal
Specificity:
CRADD Antibody detects endogenous levels of total CRADD.
RRID:
AB_2838226
Cite Format: Affinity Biosciences Cat# DF6260, RRID:AB_2838226.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:

Fold/Unfold

CASP2 and RIPK1 domain containing adaptor with death domain; Caspase and RIP adapter with death domain; Caspase and RIP adaptor with death domain; Cradd; CRADD_HUMAN; Death adaptor molecule RAIDD; Death domain containing protein CRADD; Death domain-containing protein CRADD; MGC9163; RIP associated ICH1/CED3 homologous protein with death domain; RIP associated protein with a death domain; RIP-associated protein with a death domain;

Immunogens

Immunogen:
Uniprot:

P78560(CRADD_HUMAN) >>Visit HPA database.

Gene(ID):
CRADD(8738)
Expression:
P78560 CRADD_HUMAN:

Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Description:
The protein encoded by this gene is a death domain (CARD/DD)-containing protein and has been shown to induce cell apoptosis. Through its CARD domain, this protein interacts with, and thus recruits, caspase 2/ICH1 to the cell death signal transduction complex that includes tumor necrosis factor receptor 1 (TNFR1A), RIPK1/RIP kinase, and numbers of other CARD domain-containing proteins. [provided by RefSeq, Jul 2008]
Sequence:
MEARDKQVLRSLRLELGAEVLVEGLVLQYLYQEGILTENHIQEINAQTTGLRKTMLLLDILPSRGPKAFDTFLDSLQEFPWVREKLKKAREEAMTDLPAGDRLTGIPSHILNSSPSDRQINQLAQRLGPEWEPMVLSLGLSQTDIYRCKANHPHNVQSQVVEAFIRWRQRFGKQATFQSLHNGLRAVEVDPSLLLHMLE

Predictions

Predictions:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Bovine
92
Sheep
92
Dog
92
Chicken
83
Horse
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P78560 As Substrate

Site PTM Type Enzyme
S11 Phosphorylation
T48 Phosphorylation
K53 Ubiquitination
S116 Phosphorylation
S141 Phosphorylation
K173 Ubiquitination

Research Backgrounds

Function:

Adapter protein that associates with PIDD1 and the caspase CASP2 to form the PIDDosome, a complex that activates CASP2 and triggers apoptosis. Also recruits CASP2 to the TNFR-1 signaling complex through its interaction with RIPK1 and TRADD and may play a role in the tumor necrosis factor-mediated signaling pathway.

Subcellular Location:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
Tissue Specificity:

Constitutively expressed in most tissues, with particularly high expression in adult heart, testis, liver, skeletal muscle, fetal liver and kidney.

Subunit Structure:

Forms a complex named the PIDDosome with PIDD1 and CASP2. Interacts (via Death domain) with RIPK1 (via Death domain); the interaction is direct. Interacts with TRADD. Interacts with TNFRSF1A.

Family&Domains:

The Death domain mediates the interaction with PIDD1 and the formation of a complex composed of 5 PIDD1 and 7 CRADD proteins which in turn probably recruit 7 CASP2 to form the PIDDosome (PubMed:17289572). The Death domain mediates a direct interaction with the Death domain of RIPK1 (PubMed:9044836).

The CARD domain mediates a direct interaction with CASP2.

Restrictive clause

 

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For Research Use Only.
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