PHPT1 Antibody - #DF6262
(1)
| Product: | PHPT1 Antibody |
| Catalog: | DF6262 |
| Description: | Rabbit polyclonal antibody to PHPT1 |
| Application: | WB IHC |
| Reactivity: | Human, Mouse, Rat |
| Prediction: | Pig, Zebrafish, Bovine, Sheep, Rabbit, Dog, Chicken |
| Mol.Wt.: | 14kDa; 14kD(Calculated). |
| Uniprot: | Q9NRX4 |
| RRID: | AB_2838228 |
Related Downloads
Protocols
Product Info
Source:
Rabbit
Application:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
Reactivity:
Human,Mouse,Rat
Prediction:
Pig(100%), Zebrafish(86%), Bovine(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
Clonality:
Polyclonal
Specificity:
PHPT1 Antibody detects endogenous levels of total PHPT1.
RRID:
AB_2838228
Cite Format: Affinity Biosciences Cat# DF6262, RRID:AB_2838228.
Cite Format: Affinity Biosciences Cat# DF6262, RRID:AB_2838228.
Conjugate:
Unconjugated.
Purification:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Storage:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
Alias:
Fold/Unfold
14 kDa phosphohistidine phosphatase; CGI 202; HSPC141; Phosphohistidine phosphatase 1; PHP14; PHP14_HUMAN; PHPT1; Protein janus A homolog; Protein janus-A homolog; Sex regulated protein janus a;
Immunogens
Immunogen:
Uniprot:
Gene(ID):
Expression:
Description:
PHPT1 (Phosphohistidine phosphatase 1), also known as 14 kDaphosphohistidine phosphatase, is a 125 amino acid enzyme belonging to the Janus protein family. Existing as a monomer in the cytoplasm, PHPT1 is an EDTA-insensitive phosphohistidine phosphatase. First identified in human adrenal gland, PHPT1 is highly expressed in skeletal muscle and heart, with lower expression in liver, pancreas and kidney. Overexpression of PHPT1 leads to specific phosphohistidine phosphatase activity towards phosphopeptide I, with no activity detected towards phosphotyrosine, phosphothreonine and phosphoserine peptides. PHPT1 is highly conserved among species, suggesting that it serves an essential functional role.
Sequence:
- Q9NRX4 PHP14_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MAVADLALIPDVDIDSDGVFKYVLIRVHSAPRSGAPAAESKEIVRGYKWAEYHADIYDKVSGDMQKQGCDCECLGGGRISHQSQDKKIHVYGYSMAYGPAQHAISTEKIKAKYPDYEVTWANDGY
Predictions
Predictions:
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Zebrafish
86
Xenopus
78
Horse
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9NRX4 As Substrate
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| A2 | Acetylation | Uniprot | |
| S16 | Phosphorylation | Uniprot | |
| Y22 | Phosphorylation | Uniprot | |
| S29 | Phosphorylation | Uniprot | |
| S33 | Phosphorylation | Uniprot | |
| S40 | Phosphorylation | Uniprot | |
| K41 | Ubiquitination | Uniprot | |
| Y57 | Phosphorylation | Uniprot | |
| K59 | Ubiquitination | Uniprot | |
| K66 | Ubiquitination | Uniprot | |
| Y91 | Phosphorylation | Uniprot | |
| Y93 | Phosphorylation | Uniprot | |
| S94 | Phosphorylation | Uniprot | |
| Y97 | Phosphorylation | Uniprot | |
| K112 | Ubiquitination | Uniprot | |
| Y113 | Phosphorylation | Uniprot | |
| Y116 | Phosphorylation | Uniprot | |
| Y125 | Phosphorylation | Uniprot |
Research Backgrounds
Function:
Exhibits phosphohistidine phosphatase activity.
Subcellular Location:
Cytoplasm.
Tissue Specificity:
Expressed abundantly in heart and skeletal muscle.
Subunit Structure:
Monomer.
Family&Domains:
Belongs to the janus family.
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